id
string | source
string | category
string | gene_id
string | gene_name
string | gene_symbol
string | protein_id
string | protein_name
string | sequence
string | title
string | abstract
string | full_text
string | pmid
string | doi
string | authors
string | journal
string | year
string | timestamp
string |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
23435899
|
pubmed_async
|
literature
|
Growth of the yeast Kluyveromyces marxianus CBS 6556 on different sugar combinations as sole carbon and energy source.
|
The yeast Kluyveromyces marxianus has been pointed out as a promising microorganism for a variety of industrial bioprocesses. Although genetic tools have been developed for this yeast and different potential applications have been investigated, quantitative physiological studies have rarely been reported. Here, we report and discuss the growth, substrate consumption, metabolite formation, and respiratory parameters of K. marxianus CBS 6556 during aerobic batch bioreactor cultivations, using a defined medium with different sugars as sole carbon and energy source, at 30 and 37 °C. Cultivations were carried out both on single sugars and on binary sugar mixtures. Carbon balances closed within 95 to 101 % in all experiments. Biomass and CO2 were the main products of cell metabolism, whereas by-products were always present in very low proportion (<3 % of the carbon consumed), as long as full aerobiosis was guaranteed. On all sugars tested as sole carbon and energy source (glucose, fructose, sucrose, lactose, and galactose), the maximum specific growth rate remained between 0.39 and 0.49 h(-1), except for galactose at 37 °C, which only supported growth at 0.31 h(-1). Different growth behaviors were observed on the binary sugar mixtures investigated (glucose and lactose, glucose and galactose, lactose and galactose, glucose and fructose, galactose and fructose, fructose and lactose), and the observations were in agreement with previously published data on the sugar transport systems in K. marxianus. We conclude that K. marxianus CBS 6556 does not present any special nutritional requirements; grows well in the range of 30 to 37 °C on different sugars; is capable of growing on sugar mixtures in a shorter period of time than Saccharomyces cerevisiae, which is interesting from an industrial point of view; and deviates tiny amounts of carbon towards metabolite formation, as long as full aerobiosis is maintained.
|
23435899
|
10.1007/s00253-013-4748-6
|
Gustavo Graciano Fonseca; Nuno Miguel Barbosa de Carvalho; Andreas Karoly Gombert
|
Applied microbiology and biotechnology
|
2013
|
2025-11-10T15:53:40.968708
|
|||||||
12177423
|
pubmed_async
|
literature
|
Conservation of a portion of the S. cerevisiae Ure2p prion domain that interacts with the full-length protein.
|
The [URE3] prion of Saccharomyces cerevisiae is a self-propagating inactive amyloid form of the Ure2 protein. Ure2p residues 1-65 constitute the prion domain, and the remaining C-terminal portion regulates nitrogen catabolism. We have examined the URE2 genes of wild-type isolates of S. cerevisiae and those of several pathogenic yeasts and a filamentous fungus. We find that the normal function of the S. cerevisiae Ure2p in nitrogen regulation is fully complemented by the Ure2p of Candida albicans, Candida glabrata, Candida kefyr, Candida maltosa, Saccharomyces bayanus, and Saccharomyces paradoxus, all of which have high homology in the C-terminal nitrogen regulation domain. However, there is considerable divergence of their N-terminal domains from that of Ure2p of S. cerevisiae. [URE3(Sc)] showed efficient transmission into S. cerevisiae ure2Delta cells if expressing a Ure2p of species within Saccharomyces. However, [URE3(Sc)] did not seed self-propagating inactivation of the Ure2p's from the other yeasts. When overexpressed as a fusion with green fluorescent protein, residues 5-47 of the S. cerevisiae prion domain are necessary for curing the [URE3] prion. Residues 11-39 are necessary for an inactivating interaction with the full-length Ure2p. A nearly identical region is highly conserved among many of the yeasts examined in this study, despite the wide divergence of sequences found in other parts of the N-terminal domains.
|
12177423
|
10.1073/pnas.162349599
|
Herman K Edskes; Reed B Wickner
|
Proceedings of the National Academy of Sciences of the United States of America
|
2002
|
2025-11-10T15:53:40.969022
|
|||||||
40952531
|
pubmed_async
|
literature
|
Prevalence of Candida and Other Yeasts in Vulvovaginal Infections during Pregnancy: A 10-Year Serbian Survey.
|
Candida is a common and harmless component of the vaginal microbiota, present in approximately 50% of women of reproductive age. In specific conditions like pregnancy, Candida may cause chronic or recurrent infections which significantly impair quality of life. Additionally, due to the possibility of vertical transmission, it may lead to life-threatening infections in newborns. Timely screening, suspicion and identification of the causative agent are critical determinants of patient outcomes. Candida albicans (CA) remains the most prevalent species, but other non-albicans Candida (NAC) species and other non-Candida yeast (NCY) also play an important role in vulvovaginal infections. Due to the lack of precise local epidemiological data, this study aimed to determine the 10-year prevalence of CA, NAC and NCY species (spp.) in symptomatic pregnant women in Serbia using identification Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry (MALDI-TOF MS) for species identification. The total of 2,142 cases were examined (2013-2023) and the laboratory positivity was 48.3% (n = 1,035). The prevalence of CA, NAC and NCY were 74%, 23% and 3%, respectively. Biochemical and proteomic identification methods showed 100% concordance for CA, C. krusei (Pichia kudriavzevii), C. kefyr (Kluyveromyces marxianus), C. lusitaniae (Clavispora lusitaniae), and C. zeylanoides (Pichia norvengensis). Biochemical misidentification was observed for C. tropicalis (Lodderomyces sp.), C. glabrata (Nakaseomyces glabrata), and Saccharomyces cerevisiae. A global trend highlights the importance of renamed and reclassified yeast species in vaginal infections, supporting the reconsideration of the current disease name VVC in favor of the term vulvovaginal yeast infection (VYI). The prevalence of emerging NAC and NCY species is increasing but remains underestimated. There is a need for new laboratory diagnostic guidelines to enable timely and accurate identification, as this is crucial for guiding appropriate treatment and improving outcomes.
|
40952531
|
10.1007/s11046-025-00989-9
|
Valentina Arsić Arsenijević; Vladimir Gerginić; Aleksandar Jurišić; Suzana Otaševic; Marina Ranđelović; Ljubomir Petričević
|
Mycopathologia
|
2025
|
2025-11-10T15:53:40.969355
|
|||||||
16915700
|
pubmed_async
|
literature
|
Inulin-containing biomass for ethanol production: carbohydrate extraction and ethanol fermentation.
|
The use of stalks instead of tubers as a source of carbohydrates for ethanol production has been investigated. The inulin present in the stalks of Jerusalem artichoke was extracted with water and the effect of solid-liquid ratio, temperature, and acid addition was studied and optimized in order to attain a high-fructose fermentable extract. The maximum extraction efficiency (corresponding to 35 g/L) of soluble sugars was obtained at 1/6 solid-liquid ratio. Fermentations of hydrolyzed extracts by baker's yeast and direct fermentation by an inulinase activity yeast were also performed and the potential to use this feedstock for bioethanol production assessed. The results show that the carbohydrates derived from Jerusalem artichoke stalks can be converted efficiently to ethanol by acidic hydrolysis followed by fermentation with Saccharomyces cerevisiae or by direct fermentation of inulin using Kluyveromyces marxianus strains. In this last case about 30 h to complete fermentation was required in comparison with 8-9 h obtained in experiments with S. cerevisiae growth on acid extracted juices.
|
16915700
|
10.1385/abab:132:1:922
|
Ma José Negro; Ignacio Ballesteros; Paloma Manzanares; José Miguel Oliva; Felicia Sáez; Mercedes Ballesteros
|
Applied biochemistry and biotechnology
|
2006
|
2025-11-10T15:53:40.969661
|
|||||||
17021079
|
pubmed_async
|
literature
|
Global surveillance of in vitro activity of micafungin against Candida: a comparison with caspofungin by CLSI-recommended methods.
|
Micafungin is an echinocandin antifungal agent that has recently been approved for the prevention of invasive fungal infection and the treatment of esophageal candidiasis. Prospective sentinel surveillance for the emergence of in vitro resistance to micafungin among invasive Candida sp. isolates is indicated. We determined the in vitro activity of micafungin against 2,656 invasive (bloodstream or sterile site) unique patient isolates of Candida spp. collected from 60 medical centers worldwide in 2004 and 2005. We performed antifungal susceptibility testing according to the Clinical and Laboratory Standards Institute (CLSI) M27-A2 method and used a 24-hour prominent inhibition endpoint for determination of the MIC. Caspofungin was tested in parallel against all isolates. Of 2,656 invasive Candida sp. isolates, species distribution was 55.6% Candida albicans, 14.4% Candida parapsilosis, 13.4% Candida glabrata, 10.1% Candida tropicalis, 2.4% Candida krusei, 1.7% Candida guilliermondii, 0.9% Candida lusitaniae, 0.6% Candida kefyr, and 0.9% other Candida species. Overall, micafungin was very active against Candida (MIC50/MIC at which 90% of the isolates tested are inhibited [MIC90], 0.015/1 microg/ml; 96% inhibited at a MIC of < or =1 microg/ml, 100% inhibited at a MIC of < or =2 microg/ml) and comparable to caspofungin (MIC50/MIC90, 0.03/0.25 mug/ml; 99% inhibited at a MIC of < or =2 microg/ml). Results by species, expressed as MIC50/MIC90 (micrograms per milliliter), were as follows: C. albicans, 0.015/0.03; C. glabrata, 0.015/0.015; C. tropicalis, 0.03/0.06; C. krusei, 0.06/0.12; C. kefyr, 0.06/0.06; C. parapsilosis, 1/2; C. guilliermondii, 0.5/1; C. lusitaniae, 0.12/0.25; other Candida spp., 0.25/1. Although the species distribution varied considerably among the different geographic regions, there was no difference in micafungin activity across the regions. Micafungin has excellent in vitro activity against invasive clinical isolates of Candida from centers worldwide.
|
17021079
|
10.1128/JCM.00872-06
|
M A Pfaller; L Boyken; R J Hollis; S A Messer; S Tendolkar; D J Diekema
|
Journal of clinical microbiology
|
2006
|
2025-11-10T15:53:40.969944
|
|||||||
35434416
|
pubmed_async
|
literature
|
Respiratory tract clinometry, fat thickness, haematology and productive parameters associated with direct-fed microbials used as growth promoter antibiotic alternative in weaned piglets.
|
The objective was to evaluate the effect of two probiotic yeast strains (
|
35434416
|
10.3389/fmicb.2018.03218
|
Alejandra Paola Magnoli; Julián Parada; Fátima Candelaria de la Torre; Santiago Watson; Valeria Poloni; Analía Fochesato; María Pía Martínez; María Valeria Coniglio; María Eugenia Ortiz; Lilia Cavaglieri
|
Veterinary and animal science
|
2022
|
2025-11-10T15:53:40.970286
|
|||||||
39194889
|
pubmed_async
|
literature
|
Phenotypic and Genotypic Characterization of Resistance and Virulence Markers in
|
This study aimed to determine, at the phenotypic and molecular levels, resistance and virulence markers in A total of 62 Of the total of 62 strains, 45.16% were The obtained results in
|
39194889
|
10.2147/IJN.S210449
|
Viorica Maria Corbu; Ana-Maria Georgescu; Ioana Cristina Marinas; Radu Pericleanu; Denisa Vasilica Mogos; Andreea Ștefania Dumbravă; Liliana Marinescu; Ionut Pecete; Tatiana Vassu-Dimov; Ilda Czobor Barbu; Ortansa Csutak; Denisa Ficai; Irina Gheorghe-Barbu
|
Journal of fungi (Basel, Switzerland)
|
2024
|
2025-11-10T15:53:40.970747
|
|||||||
29997753
|
pubmed_async
|
literature
|
Screening and characterization of
|
Probiotics are defined as live micro-organisms conferring a health benefit on the host. Although most probiotics are bacteria, some yeasts such as Strains were isolated on yeast glucose chloramphenicol agar medium from 205 samples and identified by morphological, physiological and biochemical assays. The effects of different conditions such as pH and temperature on the survival and growth of the isolates were studied. In addition, resistance to acidic pH (1.5, 2, 3 and 5), pepsin and different concentrations of bile salts (1%, 3% and 5%), as well as proteolytic, lipolytic and hemolytic activity of selected isolates were assessed. Finally, the best isolates were selected for investigation of their viability in samples of dairy products. 126 isolates were identified using biochemical and molecular techniques as yeast strains. Five isolates were found to have effective probiotic properties, belonging to In the
|
29997753
|
Reyhaneh Moradi; Rahim Nosrati; Hamed Zare; Tahereh Tahmasebi; Horieh Saderi; Parviz Owlia
|
Iranian journal of microbiology
|
2018
|
2025-11-10T15:53:40.971071
|
||||||||
10974568
|
pubmed_async
|
literature
|
Kluyveromyces marxianus exhibits an ancestral Saccharomyces cerevisiae genome organization downstream of ADH2.
|
In Saccharomyces cerevisiae, the alcohol dehydrogenase genes ADH1 and ADH5 are part of a duplicated block of genome, thought to originate from a genome-wide duplication posterior to the divergence from the Kluyveromyces lineage. We report here the characterization of Kluyveromyces marxianus ADH2 and the five genes found in its immediate downstream region, MRPS9, YOL087C, RPB5, RIB7 and SPP381. The order of these six genes reflects the structure of the ancestral S. cerevisiae genome before the duplication that formed the blocks including ADH1 on chromosome XV and ADH5 on chromosome II, indicating these ADH genes share a direct ancestor. On the one hand, the two genes found immediately downstream of KmADH2 are located, for the first, downstream ADH5 and, for the second, downstream ADH1 in S. cerevisiae. On the other hand, the order of the paralogs included in the blocks of ADH1 and ADH5 in S. cerevisiae suggests that two of them have been inverted within one block after its formation, and that inversion is confirmed by the gene order observed in K. marxianus.
|
10974568
|
10.1016/s0378-1119(00)00310-3
|
J M Ladrière; I Georis; M Guérineau; J Vandenhaute
|
Gene
|
2000
|
2025-11-10T15:53:40.971352
|
|||||||
30221316
|
pubmed_async
|
literature
|
Application of the Severity Factor and HMF Removal of Red Macroalgae Gracilaria verrucosa to Production of Bioethanol by Pichia stipitis and Kluyveromyces marxianus with Adaptive Evolution.
|
Gracilaria verrucosa, red seaweed, is a promising biomass for bioethanol production due to its high carbohydrate content. The optimal hyper thermal (HT) acid hydrolysis conditions are 12% (w/v) G. verrucosa with 0.2 M H
|
30221316
|
10.1007/s12010-018-2888-y
|
Pailin Sukwong; In Yung Sunwoo; Min Ju Lee; Chae Hun Ra; Gwi-Taek Jeong; Sung-Koo Kim
|
Applied biochemistry and biotechnology
|
2019
|
2025-11-10T15:53:40.971622
|
|||||||
35879830
|
pubmed_async
|
literature
|
Yeasts from Iranian traditional milk kefir samples: isolation, molecular identification and their potential probiotic properties.
|
Milk kefir is a fermented dairy product with numerous attributed health benefits due to the presence of a complex eukaryotic and prokaryotic microbiota. In this study, a total number of 26 yeast isolates were obtained from eight kefir samples from three different cities of Iran. The isolates belonged to Kluyveromyces marxianus, Saccharomyces cerevisiae, Pichia fermentans and P. kudriavzevii. The potential probiotic characteristics of the isolates were evaluated based on their ability to tolerate the stimulated condition of the gastrointestinal tract. In addition, hemolytic activity, adherence to different solvents, auto-aggregation, adhesion to the epithelial intestine-derived cells and antimicrobial activity of the selected isolates were evaluated. Overall, four yeast strains (three strains of S. cerevisiae and one strain of P. fermentans) showed resistance and survival ability against the gastrointestinal physiological conditions including acidic pH, presence of bile salt and digestive enzymes. They were able to grow at 37°C and had the capacity to adhere to epithelial intestine-derived cells. These results suggest that the selected strains can be proper candidates as probiotic yeast strains for the development of novel functional foods.
|
35879830
|
10.1111/lam.13794
|
B Rahmani; N Alimadadi; B Attaran; S Nasr
|
Letters in applied microbiology
|
2022
|
2025-11-10T15:53:40.971938
|
|||||||
38663544
|
pubmed_async
|
literature
|
GMMA Can Stabilize Proteins Across Different Functional Constraints.
|
Stabilizing proteins without otherwise hampering their function is a central task in protein engineering and design. PYR1 is a plant hormone receptor that has been engineered to bind diverse small molecule ligands. We sought a set of generalized mutations that would provide stability without affecting functionality for PYR1 variants with diverse ligand-binding capabilities. To do this we used a global multi-mutant analysis (GMMA) approach, which can identify substitutions that have stabilizing effects and do not lower function. GMMA has the added benefit of finding substitutions that are stabilizing in different sequence contexts and we hypothesized that applying GMMA to PYR1 with different functionalities would identify this set of generalized mutations. Indeed, conducting FACS and deep sequencing of libraries for PYR1 variants with two different functionalities and applying a GMMA analysis identified 5 substitutions that, when inserted into four PYR1 variants that each bind a unique ligand, provided an increase of 2-6 °C in thermal inactivation temperature and no decrease in functionality.
|
38663544
|
10.1016/j.jmb.2024.168586
|
Nicolas Daffern; Kristoffer E Johansson; Zachary T Baumer; Nicholas R Robertson; Janty Woojuh; Matthew A Bedewitz; Zoë Davis; Ian Wheeldon; Sean R Cutler; Kresten Lindorff-Larsen; Timothy A Whitehead
|
Journal of molecular biology
|
2024
|
2025-11-10T15:53:40.972248
|
|||||||
37525429
|
pubmed_async
|
literature
|
Optimal Extraction and Deproteinization Method for Mannoprotein Purification from Kluyveromyces marxianus.
|
Mannoproteins, mannose-glycosylated proteins, play an important role in biological processes and have various applications in industries. Several methods have been already used for the extraction of mannoproteins from yeast cell-wall. The aim of this study was to evaluate the extraction and deproteinization of mannan oligosaccharide from the Kluyveromyces (K.) marxianus mannoprotein. To acquire crude mannan oligosaccharides, K. marxianus mannoproteins were deproteinized by the Sevage, trichloroacetic acid, and hydrochloric acid (HCL) methods. Total nitrogen, crude protein content, fat, carbohydrate and ash content were measured according to the monograph prepared by the meeting of the Joint FAO/WHO Expert Committee and standard. Mannan oligosaccharide loss, percentage of deproteinization, and chemical composition of the product were assessed to check the proficiency of different methods. Highly purified (95.4%) mannan oligosaccharide with the highest deproteinization (97.33 ± 0.4%) and mannan oligosaccharide loss (25.1 ± 0.6%) were obtained following HCl method. HCl, was the most appropriate deproteinization method for the removal of impurities. This preliminary data will support future studies to design scale-up procedures.
|
37525429
|
10.61186/ibj.27.5.320
|
Ashraf Hajhosseini; Anousheh Sharifan; Zohreh Eftekhari; Ariana Alavi; Delaram Doroud
|
Iranian biomedical journal
|
2023
|
2025-11-10T15:53:40.972537
|
|||||||
23604526
|
pubmed_async
|
literature
|
Evaluation of hardboard manufacturing process wastewater as a feedstream for ethanol production.
|
Waste streams from the wood processing industry can serve as feedstream for ethanol production from biomass residues. Hardboard manufacturing process wastewater (HPW) was evaluated on the basis of monomeric sugar recovery and fermentability as a novel feedstream for ethanol production. Dilute acid hydrolysis, coupled with concentration of the wastewater resulted in a hydrolysate with 66 g/l total fermentable sugars. As xylose accounted for 53 % of the total sugars, native xylose-fermenting yeasts were evaluated for their ability to produce ethanol from the hydrolysate. The strains selected were, in decreasing order by ethanol yields from xylose (Y p/s, based on consumed sugars), Scheffersomyces stipitis ATCC 58785 (CBS 6054), Pachysolen tannophilus ATCC 60393, and Kluyveromyces marxianus ATCC 46537. The yeasts were compared on the basis of substrate utilization and ethanol yield during fermentations of the hydrolysate, measured using an HPLC. S. stipitis, P. tannophilus, and K. marxianus produced 0.34, 0.31, and 0.36 g/g, respectively. The yeasts were able to utilize between 58 and 75 % of the available substrate. S. stipitis outperformed the other yeast during the fermentation of the hydrolysate; consuming the highest concentration of available substrate and producing the highest ethanol concentration in 72 h. Due to its high sugar content and low inhibitor levels after hydrolysis, it was concluded that HPW is a suitable feedstream for ethanol production by S. stipitis.
|
23604526
|
10.1007/s10295-013-1272-8
|
Stephanie Groves; Jifei Liu; David Shonnard; Susan Bagley
|
Journal of industrial microbiology & biotechnology
|
2013
|
2025-11-10T15:53:40.972842
|
|||||||
27611676
|
pubmed_async
|
literature
|
A Kluyveromyces marxianus 2-deoxyglucose-resistant mutant with enhanced activity of xylose utilization.
|
Thermotolerant ethanologenic yeast Kluyveromyces marxianus is capable of fermenting various sugars including xylose but glucose represses to hamper the utilization of other sugars. To acquire glucose repression-defective strains, 33 isolates as 2-deoxyglucose (2-DOG)-resistant mutants were acquired from about 100 colonies grown on plates containing 2-DOG, which were derived from an efficient strain DMKU 3-1042. According to the characteristics of sugar consumption abilities and cell growth and ethanol accumulation along with cultivation time, they were classified into three groups. The first group (3 isolates) utilized glucose and xylose in similar patterns along with cultivation to those of the parental strain, presumably due to reduction of the uptake of 2-DOG or enhancement of its export. The second group (29 isolates) showed greatly delayed utilization of glucose, presumably by reduction of the uptake or initial catabolism of glucose. The last group, only one isolate, showed enhanced utilization ability of xylose in the presence of glucose. Further analysis revealed that the isolate had a single nucleotide mutation to cause amino acid substitution (G270S) in RAG5 encoding hexokinase and exhibited very low activity of the enzyme. The possible mechanism of defectiveness of glucose repression in the mutant is discussed in this paper. [Int Microbiol 18(4):235-244 (2015)].
|
27611676
|
10.2436/20.1501.01.255
|
Suprayogi Suprayogi; Minh T Nguyen; Noppon Lertwattanasakul; Nadchanok Rodrussamee; Savitree Limtong; Tomoyuki Kosaka; Mamoru Yamada
|
International microbiology : the official journal of the Spanish Society for Microbiology
|
2015
|
2025-11-10T15:53:40.973132
|
|||||||
8714399
|
pubmed_async
|
literature
|
[The antibiotic properties of the phytotoxic metabolites of Botrytis cinerea Pers].
|
Antibiotic properties of substances of a phytotoxic complex from Botrytis cinerea have been studied for a number of phytopathogenic bacteria, phytopathogenic and toxigenic fungi as well as saprophytic yeast strains. High fungistatic activity of preparations of phytotoxic metabolites (PTM) has been stated for Dendrodochium toxicum, Myrothecium verrucaria, M. roridum, Aspergillus fumigatus, Penicillium urticae, agents of heavy human and cattle mycotoxicoses. The studied representatives of phytopathogenic fusaria differed significantly in sensitivity to PTM: all the strains studied of the species from section Discolor (Fusarium gibbosum 54,624 and 51,463, F. graminearum 108,269, F. sambucinum 54,968, F. culmorum 54,951) were actively inhibited by the PTM concentrations studied; F. sporotrichiella 52,290 proved to be insensitive to them; the strains studied of the species from sections Elegans (F. oxysporum 55,715, F. moniliforme 54,262) and Martiella (F. javanicum 54,075, F. solani 54,719) possessed different sensitivity to PTM. The overwhelming majority of the yeast strains proved to be resistant to the PTM concentrations studied, except for the strains of Trichosporon cutaneum. In this connection the fact of high sensitivity of one of the strains of Kluyveromyces marxianus var. marxianus to B. cinerea metabolites arouses great interest. The strains of studied phytopathogenic bacteria (Erwinia carotovora subsp. carotovora 8,982 and Clavibacter michiganense 13) were sensitive to higher concentrations of PTM.
|
8714399
|
I G Rubezhniak; L P Troshin; A M Zaĭchenko
|
Mikrobiolohichnyi zhurnal (Kiev, Ukraine : 1993)
|
1995
|
2025-11-10T15:53:40.973393
|
||||||||
16233566
|
pubmed_async
|
literature
|
Continuous production of pectinase by immobilized yeast cells on spent grains.
|
A yeast strain secreting endopolygalacturonase was used in this work to study the possibility of continuous production of this enzyme. It is a feasible and interesting alternative to fungal batch production essentially due to the specificity of the type of pectinase excreted by Kluyveromyces marxianus CCT 3172, to the lower broth viscosity and to the easier downstream operations. In order to increase the reactors' productivity, a cellulosic carrier obtained from barley spent grains was tested as an immobilization support. Two types of reactors were studied for pectinase production using glucose as a carbon and energy source--a continuous stirred tank reactor (CSTR) and a packed bed reactor (PBR) with recycled flow. The highest value for pectinase volumetric productivity (P(V)=0.98 U ml(-1) h(-1)) was achieved in the PBR for D=0.40 h(-1), a glucose concentration on the inlet of S(in)=20 g l(-1), and a biomass load in the support of X(i)=0.225 g g(-1). The results demonstrate the attractiveness of the packed bed system for pectinase production.
|
16233566
|
10.1016/S1389-1723(04)70142-5
|
Catarina Almeida; Tomás Brányik; Pedro Moradas-Ferreira; José Teixeira
|
Journal of bioscience and bioengineering
|
2003
|
2025-11-10T15:53:40.973673
|
|||||||
28181081
|
pubmed_async
|
literature
|
Genetic improvement of xylose metabolism by enhancing the expression of pentose phosphate pathway genes in Saccharomyces cerevisiae IR-2 for high-temperature ethanol production.
|
The pentose phosphate pathway (PPP) plays an important role in the efficiency of xylose fermentation during cellulosic ethanol production. In simultaneous saccharification and co-fermentation (SSCF), the optimal temperature for cellulase hydrolysis of lignocellulose is much higher than that of fermentation. Successful use of SSCF requires optimization of the expression of PPP genes at elevated temperatures. This study examined the combinatorial expression of PPP genes at high temperature. The results revealed that over-expression of TAL1 and TKL1 in Saccharomyces cerevisiae (S. cerevisiae) at 30 °C and over-expression of all PPP genes at 36 °C resulted in the highest ethanol productivities. Furthermore, combinatorial over-expression of PPP genes derived from S. cerevisiae and a thermostable yeast Kluyveromyces marxianus allowed the strain to ferment xylose with ethanol productivity of 0.51 g/L/h, even at 38 °C. These results clearly demonstrate that xylose metabolism can be improved by the utilization of appropriate combinations of thermostable PPP genes in high-temperature production of ethanol.
|
28181081
|
10.1007/s10295-017-1912-5
|
Yosuke Kobayashi; Takehiko Sahara; Toshihiro Suzuki; Saori Kamachi; Akinori Matsushika; Tamotsu Hoshino; Satoru Ohgiya; Yoichi Kamagata; Kazuhiro E Fujimori
|
Journal of industrial microbiology & biotechnology
|
2017
|
2025-11-10T15:53:40.973988
|
|||||||
30151682
|
pubmed_async
|
literature
|
Systematic optimization of gene expression of pentose phosphate pathway enhances ethanol production from a glucose/xylose mixed medium in a recombinant Saccharomyces cerevisiae.
|
The pentose phosphate pathway (PPP) plays an important role in the synthesis of ribonucleotides and aromatic amino acids. During bioethanol production from cellulosic biomass composed mainly of D-glucose and D-xylose, the PPP is also involved in xylose metabolism by engineered Saccharomyces cerevisiae. Although the activities and thermostabilities of the four PPP enzymes (transaldolase: TAL1, transketolase: TKL1, ribose-5-phosphate ketol-isomerase: RKI1 and D-ribulose-5-phosphate 3-epimerase: RPE1) can affect the efficiency of cellulosic ethanol production at high temperatures, little is known about the suitable expression levels of these PPP genes. Here, we overexpressed PPP genes from S. cerevisiae and the thermotolerant yeast Kluyveromyces marxianus either singly or in combination in recombinant yeast strains harboring a mutant of xylose isomerase (XI) and evaluated xylose consumption and ethanol production of these yeast transformants in glucose/xylose mixed media at 36 °C. Among the PPP genes examined, we found that: (1) strains that overexpressed S. cerevisiae TKL1 exhibited the highest rate of xylose consumption relative to strains that overexpressed other PPP genes alone; (2) overexpression of RKI1 and TAL1 derived from K. marxianus with S. cerevisiae TKL1 increased the xylose consumption rate by 1.87-fold at 24 h relative to the control strain (from 0.55 to 1.03 g/L/h); (3) the strains with XI showed higher ethanol yield than strains with xylose reductase and xylitol dehydrogenase and (4) PHO13 disruption did not improve xylose assimilation under the experimental conditions. Together these results indicated that optimization of PPP activity improves xylose metabolism in genetically engineered yeast strains, which could be useful for commercial production of ethanol from cellulosic material.
|
30151682
|
10.1186/1754-6834-3-24
|
Yosuke Kobayashi; Takehiko Sahara; Satoru Ohgiya; Yoichi Kamagata; Kazuhiro E Fujimori
|
AMB Express
|
2018
|
2025-11-10T15:53:40.974315
|
|||||||
18551478
|
pubmed_async
|
literature
|
Ethanol production from fodder beets.
|
Various yeasts such as two strains of Saccharomyces cerevisiae, Saccharomyces diastaticus, and Kluyveromyces marxianus were investigated for their ability to ferment fodder beet juice to alcohol. Juice extracted from fodder beet roots without any additives was used as a fermentation substrate. The fermentation kinetic parameters were determined and compared for each species of yeast tested. The best species for fodder beet juice fermentation was chosen and products obtained by fermentation of one hectare of fodder beet plants are given.
|
18551478
|
10.1002/bit.260250705
|
N Kosaric; A Wieczorek; S Kliza
|
Biotechnology and bioengineering
|
1983
|
2025-11-10T15:53:40.974606
|
|||||||
39635413
|
pubmed_async
|
literature
|
In lambs, weaning imposes stress that can contribute to impaired rumen epithelial barrier functionality and immunological dysregulation. In this study, the effects of a yeast co-culture consisting of
|
39635413
|
10.1016/j.aninu.2024.06.005
|
Zixuan Xu; Lan Yang; Hui Chen; Shixiong Liu; Xueqiang Li; Songjian Li; Chun Ying; Xiao Li; Rui Du; Dacheng Liu
|
Animal nutrition (Zhongguo xu mu shou yi xue hui)
|
2024
|
2025-11-10T15:53:40.974998
|
||||||||
33072642
|
pubmed_async
|
literature
|
Frequency of
|
In this study, sampling was done from the oral cavity of 83 addicts who referred to Addiction Treatment Centers in Isfahan, Iran, using moist sterile swab. The presence of yeast on the direct microscope slides of 58 samples was confirmed. To carry out culture and the primary identification, Sabouraud dextrose agar medium with chloramphenicol as well as HiCrome Out of 93 Compared to the studies conducted on the oral cavity of healthy controls, smoking certain drugs can have a significant effect on the presence and frequency of Candida species, particularly
|
33072642
|
10.4103/abr.abr_38_20
|
Parastoo Hassani Abharian; Parvin Dehghan; Peyman Hassani-Abharian; Zahra Jabalameli
|
Advanced biomedical research
|
2020
|
2025-11-10T15:53:40.975298
|
|||||||
28360937
|
pubmed_async
|
literature
|
Enhanced fermentative performance under stresses of multiple lignocellulose-derived inhibitors by overexpression of a typical 2-Cys peroxiredoxin from
|
Bioethanol from lignocellulosic materials is of great significance to the production of renewable fuels due to its wide sources. However, multiple inhibitors generated from pretreatments represent great challenges for its industrial-scale fermentation. Despite the complex toxicity mechanisms, lignocellulose-derived inhibitors have been reported to be related to the levels of intracellular reactive oxygen species (ROS), which makes oxidoreductase a potential target for the enhancement of the tolerance of yeasts to these inhibitors. A typical 2-Cys peroxiredoxin from A new functional gene
|
28360937
|
10.1002/bit.20221
|
Jiaoqi Gao; Hualiang Feng; Wenjie Yuan; Yimin Li; Shengbo Hou; Shijun Zhong; Fengwu Bai
|
Biotechnology for biofuels
|
2017
|
2025-11-10T15:53:40.975606
|
|||||||
22682742
|
pubmed_async
|
literature
|
Structural insights into Atg10-mediated formation of the autophagy-essential Atg12-Atg5 conjugate.
|
The Atg12-Atg5 conjugate, which is formed by an ubiquitin-like conjugation system, is essential to autophagosome formation, a central event in autophagy. Despite its importance, the molecular mechanism of the Atg12-Atg5 conjugate formation has not been elucidated. Here, we report the solution and crystal structures of Atg10 and Atg5 homologs from Kluyveromyces marxianus (Km), a thermotolerant yeast. KmAtg10 comprises an E2-core fold with characteristic accessories, including two β strands, whereas KmAtg5 has two ubiquitin-like domains and a helical domain. The nuclear magnetic resonance experiments, mutational analyses, and crosslinking experiments showed that KmAtg10 directly recognizes KmAtg5, especially its C-terminal ubiquitin-like domain, by its characteristic two β strands. Kinetic analysis suggests that Tyr56 and Asn114 of KmAtg10 may place the side chain of KmAtg5 Lys145 into the optimal orientation for its conjugation reaction with Atg12. These structural features enable Atg10 to mediate the formation of the Atg12-Atg5 conjugate without a specific E3 enzyme.
|
22682742
|
10.1016/j.str.2012.04.018
|
Masaya Yamaguchi; Nobuo N Noda; Hayashi Yamamoto; Takayuki Shima; Hiroyuki Kumeta; Yoshihiro Kobashigawa; Rinji Akada; Yoshinori Ohsumi; Fuyuhiko Inagaki
|
Structure (London, England : 1993)
|
2012
|
2025-11-10T15:53:40.975939
|
|||||||
30254120
|
pubmed_async
|
literature
|
Engineering Kluyveromyces marxianus as a Robust Synthetic Biology Platform Host.
|
Throughout history, the yeast
|
30254120
|
10.1093/emboj/16.20.6171
|
Paul Cernak; Raissa Estrela; Snigdha Poddar; Jeffrey M Skerker; Ya-Fang Cheng; Annika K Carlson; Berling Chen; Victoria M Glynn; Monique Furlan; Owen W Ryan; Marie K Donnelly; Adam P Arkin; John W Taylor; Jamie H D Cate
|
mBio
|
2018
|
2025-11-10T15:53:40.976515
|
|||||||
37667920
|
pubmed_async
|
literature
|
Distribution of fungemia agents in five years and antifungal resistance.
|
Recent research has suggested that fungemia may demonstrate an epidemiologic shift in etiologic agents. This study focuses on the agents causing fungemia and antifungal resistance in a tertiary hospital. We evaluated all-age fungemia cases admitted to Balikesir Ataturk City Hospital in 2017-2021. Blood cultures (BC) were studied using BacT/Alert® 3D (bioMérieux, Marcyl'Etoile, France) and Render BC128 System (Render Biotech Co. Ltd., Shenzhen, China). On the data, we explored only the first fungal positive samples or the first isolates in different episodes of the same patients. Upon The Clinical and Laboratory Standards Institute (CLSI) disk diffusion guidelines, conventional methods and the Phoenix™ 100 System (Becton Dickinson, Franklin Lakes, NJ, USA) were utilized for antifungal susceptibility identifications. The findings showed that 325 (0.84%) of 38,682 BC sets were positive for fungal growth. Except for four cases (1.2%) [Saprochaete capitata (n = 2); Trichosporon asahii (n = 1), and Saccharomyces cerevisiae (n = 1)], all positive cases yielded Candida spp. (98.8%) growth. In these patients, the following Candida spp. were isolated: Candida albicans complex (n = 155; 47.7%), Candida parapsilosis complex (n = 127; 39.1%), Candida glabrata complex (n = 19; 5.85%), Candida tropicalis (n = 12; 3.7%), Candida kefyr (n = 5; 1.54%), Candida krusei (n = 2; 0.62%), and Candida guilliermondii complex (n = 1; 0.31%). We also realized that while none of the Candida spp. had echinocandin resistance, 8 C. parapsilosis complex isolates were resistant to fluconazole, and 17 C. parapsilosis complex and 2 C. tropicalis isolates were susceptible dose-dependent to fluconazole. In brief, antifungal resistance is more likely to restrict therapeutic options, albeit it is, fortunately, not prevalent in Turkey despite a few recent reports. Yet, a robust detection or management of antifungal resistance requires species-level identification and strict compliance with relevant management guidelines. Besides, challenges in research may be compensated with a national data set built with data from local laboratories.
|
37667920
|
10.26355/eurrev_202308_33395
|
A K Sig; A Çetin-Duran; T Kula-Atik
|
European review for medical and pharmacological sciences
|
2023
|
2025-11-10T15:53:40.976807
|
|||||||
33762022
|
pubmed_async
|
literature
|
Cross-kingdom inhibition of bacterial virulence and communication by probiotic yeast metabolites.
|
Probiotic milk-fermented microorganism mixtures (e.g., yogurt, kefir) are perceived as contributing to human health, and possibly capable of protecting against bacterial infections. Co-existence of probiotic microorganisms are likely maintained via complex biomolecular mechanisms, secreted metabolites mediating cell-cell communication, and other yet-unknown biochemical pathways. In particular, deciphering molecular mechanisms by which probiotic microorganisms inhibit proliferation of pathogenic bacteria would be highly important for understanding both the potential benefits of probiotic foods as well as maintenance of healthy gut microbiome. The microbiome of a unique milk-fermented microorganism mixture was determined, revealing a predominance of the fungus Kluyveromyces marxianus. We further identified a new fungus-secreted metabolite-tryptophol acetate-which inhibits bacterial communication and virulence. We discovered that tryptophol acetate blocks quorum sensing (QS) of several Gram-negative bacteria, particularly Vibrio cholerae, a prominent gut pathogen. Notably, this is the first report of tryptophol acetate production by a yeast and role of the molecule as a signaling agent. Furthermore, mechanisms underscoring the anti-QS and anti-virulence activities of tryptophol acetate were elucidated, specifically down- or upregulation of distinct genes associated with V. cholerae QS and virulence pathways. This study illuminates a yet-unrecognized mechanism for cross-kingdom inhibition of pathogenic bacteria cell-cell communication in a probiotic microorganism mixture. A newly identified fungus-secreted molecule-tryptophol acetate-was shown to disrupt quorum sensing pathways of the human gut pathogen V. cholerae. Cross-kingdom interference in quorum sensing may play important roles in enabling microorganism co-existence in multi-population environments, such as probiotic foods and the gut microbiome. This discovery may account for anti-virulence properties of the human microbiome and could aid elucidating health benefits of probiotic products against bacterially associated diseases. Video Abstract.
|
33762022
|
10.1021/acs.orglett.5b01024
|
Orit Malka; Dorin Kalson; Karin Yaniv; Reut Shafir; Manikandan Rajendran; Oshrit Ben-David; Ariel Kushmaro; Michael M Meijler; Raz Jelinek
|
Microbiome
|
2021
|
2025-11-10T15:53:40.977199
|
|||||||
23338601
|
pubmed_async
|
literature
|
Essentiality of respiratory activity for pentose utilization in thermotolerant yeast Kluyveromyces marxianus DMKU 3-1042.
|
By random integrative mutagenesis with a kanMX4 cassette in Kluyveromyces marxianus DMKU 3-1042, we obtained three mutants of COX15, ATP25 and CYC3 encoding a cytochrome oxidase assembly factor (singleton), a transcription factor required for assembly of the Atp9p subunit of mitochondrial ATP synthase and cytochrome c heme lyase, respectively, as mutants lacking growth capability on xylose and/or arabinose. They exhibited incapability of growth on non-fermentable carbon sources, such as acetate or glycerol, and thermosensitiveness. Their biomass formation in glucose medium was reduced, but ethanol yields were increased with a high ethanol level in the medium, compared to those of the parental strain. Experiments with respiratory inhibitors showed that cox15 and cyc3, but not atp25, were able to grow in glucose medium containing antimycin A and that the atp25 mutant was KCN-resistant. Activities of NADH and ubiquinol oxidases in membrane fractions of each mutant became a half of that of the parent and negligible, respectively, and their remaining NADH oxidase activities were found to be resistant to KCN. Absolute absorption spectral analysis revealed that the peak corresponding to a + a 3 was very small in atp25 and negligible in cox15 and cyc3. These findings suggest that the K. marxianus strain possesses an alternative KCN-resistant oxidase that is located between primary dehydrogenases and the ubiquinone pool and that the respiratory activity is essential for utilization of pentoses.
|
23338601
|
10.1007/s10482-012-9874-0
|
Noppon Lertwattanasakul; Masayuki Murata; Nadchanok Rodrussamee; Savitree Limtong; Tomoyuki Kosaka; Mamoru Yamada
|
Antonie van Leeuwenhoek
|
2013
|
2025-11-10T15:53:40.977503
|
|||||||
40086987
|
pubmed_async
|
literature
|
Degradation of patulin by a yeast strain Kluyveromyces marxianus XZ1 and its mechanism.
|
Patulin (PAT) produced by genus of Penicillium spp attracted more and more concern in view of its widespread contamination in food and toxic effects, which has also promoted the research on the reduction of PAT contamination in food. The use of yeast to remove PAT in food is innovative and promising. In this study, we used the yeast Kluyveromyces marxianus XZ1 to degrade PAT, which can remove 90% of PAT (10 μg/mL) within 48 h. XZ1 exhibits high degradation effect on PAT under the conditions of pH range of 3-6, temperatures of 28-37 °C, and initial PAT concentrations below 50 μg/mL. PAT removing by XZ1 was carried out by intracellular enzymes. XZ1 or intracellular enzyme was able to remove 100% of 10 μg/mL PAT in raw apple juice or commercial apple juice within 60 h. Patulin oxidoreductase (KmPAO) was identified as a potential PTA-degrading enzymes, which degrade PAT to form ascladiol. The degradation products of PAT by XZ1 were identified as ascladiol and desoxypatulinic acid, which was then complete degraded to form unknown final degradation products. Toxic analyses on Caco-2 cells showed that the ascladiol, desoxypatulinic acid and the final degradation products were significantly less toxic compared to PAT, which was mainly manifested in less influence on cell vitality, cell integrity and reactive oxygen species accumulation compared to PAT. Finally, the results revealed the PAT degradation enzyme, as well as the safety of the degradation products, which provide basis for the future application of this yeast to decontamination of PAT in food.
|
40086987
|
10.1016/j.fm.2025.104758
|
Zihan Zhang; Jiang Li; Yiran Yang; Qinghua Gong; Huaxiang Li; Shengqi Rao; Xiangfeng Zheng; Zhenquan Yang
|
Food microbiology
|
2025
|
2025-11-10T15:53:40.977815
|
|||||||
27245756
|
pubmed_async
|
literature
|
Identification and antifungal susceptibility of Candida species isolated from bloodstream infections in Konya, Turkey.
|
In this study, our aim was to identify Candida species isolated from bloodstream infections and to determine their susceptibilities to various antifungal agents to demonstrate the local resistance profiles and to guide empirical treatment for clinicians. Two hundred Candida isolates (95 Candida albicans, 105 non-albicans Candida strains) were included in the study. Candida species were identified by conventional, biochemical and molecular methods. Antifungal susceptibility tests for amphotericin B, fluconazole, voriconazole, posaconazole, caspofungin and anidulafungin were performed with broth microdilution method according to the Clinical and Laboratory Standards Institute M27-A3 document. Of the 200 Candida strains, the most prevalent species were C. albicans (47.5 %), Candida glabrata (18.0 %) and Candida parapsilosis complex (14.0 %). All Candida species except for three (1.5 %) Candida kefyr strains were susceptible to amphotericin B. Only one (2.8 %) C. glabrata was resistant to fluconazole (MIC ≥ 64 μg/ml), and the others (97.2 %) exhibited dose-dependent susceptibility. All species, but C. glabrata strains, were susceptible to fluconazole. Resistance to voriconazole, posaconazole, caspofungin and anidulafungin was not detected in any strain. Candida albicans were susceptible to all antifungal drugs. Three C. kefyr strains were resistant to amphotericin B. Only one C. glabrata was resistant to fluconazole. All the strains were susceptible to voriconazole, posaconazole, caspofungin and anidulafungin. In vitro antifungal susceptibility tests should be performed to select of appropriate and effective antifungal therapy, and monitor the development of resistance.
|
27245756
|
10.2165/11585270-000000000-00000
|
Hatice Turk Dagi; Duygu Findik; Cigdem Senkeles; Ugur Arslan
|
Annals of clinical microbiology and antimicrobials
|
2016
|
2025-11-10T15:53:40.978138
|
|||||||
9826189
|
pubmed_async
|
literature
|
UDP-galactose 4-epimerase from Kluyveromyces fragilis: reconstitution of holoenzyme structure after dissociation with parachloromercuribenzoate.
|
UDP-galactose 4-epimerase from yeast Kluyveromyces fragilis (Kluyveromyces marxianus var. marxianus) is a homodimer of molecular mass 75 kDa/subunit and has one mol NAD firmly bound/dimer. The pathway for the assembly of the holoenzyme structure has been studied after dissociating the native epimerase with p-chloromercuribenzoate into inactive mercurated monomers. The process of dissociation was not associated with unfolding of the molecules. Reconstitution of the functional holoenzyme was done by reduction with dithiothreitol and addition of extra NAD. The reaction was thus followed to monitor maturation of the enzyme from the folded monomeric state. The reconstituted enzyme was similar to the native enzyme in terms of a number of physiochemical properties such as secondary, tertiary and quarternary structures, Km for the substrate UDP-galactose, reductive inhibition, interaction with the fluorophore 1-anilino 8-naphthalene sulphonic acid (ANS), etc. Reconstitution under low ionic strength buffer (I = 0.011) shows that the presence of NAD is essential for the formation of a dimeric structure. However, dimeric apoenzyme could also be stabilized under high ionic strength buffer (I = 0.1). Reactivation was strongly dependent on pH, being most effective at pH 8.1. Kinetic evidence suggested that, at low ionic strength, assembly of NAD over dimeric apoenzyme is the rate-limiting step in expressing catalytic activity. This process has a low energy of activation of 27.2 kJ/mol.
|
9826189
|
10.1046/j.1432-1327.1998.2570427.x
|
S Majumdar; H Bhattacharjee; D Bhattacharyya; A Bhaduri
|
European journal of biochemistry
|
1998
|
2025-11-10T15:53:40.978425
|
|||||||
22112913
|
pubmed_async
|
literature
|
Modeling of an integrated fermentation/membrane extraction process for the production of 2-phenylethanol and 2-phenylethylacetate.
|
An unstructured model for an integrated fermentation/membrane extraction process for the production of the aroma compounds 2-phenylethanol and 2-phenylethylacetate by Kluyveromyces marxianus CBS 600 was developed. The extent to which this model, based only on data from the conventional fermentation and separation processes, provided an estimation of the integrated process was evaluated. The effect of product inhibition on specific growth rate and on biomass yield by both aroma compounds was approximated by multivariate regression. Simulations of the respective submodels for fermentation and the separation process matched well with experimental results. With respect to the in situ product removal (ISPR) process, the effect of reduced product inhibition due to product removal on specific growth rate and biomass yield was predicted adequately by the model simulations. Overall product yields were increased considerably in this process (4.0 g/L 2-PE+2-PEA vs. 1.4 g/L in conventional fermentation) and were even higher than predicted by the model. To describe the effect of product concentration on product formation itself, the model was extended using results from the conventional and the ISPR process, thus agreement between model and experimental data improved notably. Therefore, this model can be a useful tool for the development and optimization of an efficient integrated bioprocess.
|
22112913
|
10.1016/j.enzmictec.2010.12.003
|
Philipp Adler; Thorsten Hugen; Marzena Wiewiora; Benno Kunz
|
Enzyme and microbial technology
|
2011
|
2025-11-10T15:53:40.978735
|
|||||||
24788328
|
pubmed_async
|
literature
|
Perspectives for the biotechnological production of ethyl acetate by yeasts.
|
Ethyl acetate is an environmentally friendly solvent with many industrial applications. The production of ethyl acetate currently proceeds by energy-intensive petrochemical processes which are based on natural gas and crude oil without exception. Microbial synthesis of ethyl acetate could become an interesting alternative. The formation of esters as aroma compounds in food has been repeatedly reviewed, but a survey which deals with microbial synthesis of ethyl acetate as a bulk product is missing. The ability of yeasts for producing larger amounts of this ester is known for a long time. In the past, this potential was mainly of scientific interest, but in the future, it could be applied to large-scale ester production from renewable raw materials. Pichia anomala, Candida utilis, and Kluyveromyces marxianus are yeasts which convert sugar into ethyl acetate with a high yield where the latter is the most promising one. Special attention was paid to the mechanism of ester synthesis including regulatory aspects and to the maximum and expectable yield. Synthesis of much ethyl acetate requires oxygen which is usually supplied by aeration. Ethyl acetate is highly volatile so that aeration results in its phase transfer and stripping. This stripping process cannot be avoided but requires adequate handling during experimentation and offers a chance for a cost-efficient process-integrated recovery of the synthesized ester.
|
24788328
|
10.1007/s00253-014-5765-9
|
Christian Löser; Thanet Urit; Thomas Bley
|
Applied microbiology and biotechnology
|
2014
|
2025-11-10T15:53:40.979032
|
|||||||
19198767
|
pubmed_async
|
literature
|
Cell disruption optimization and covalent immobilization of beta-D-galactosidase from Kluyveromyces marxianus YW-1 for lactose hydrolysis in milk.
|
beta-D-galactosidase (EC 3.2.1.23) from Kluyveromyces marxianus YW-1, an isolate from whey, has been studied in terms of cell disruption to liberate the useful enzyme. The enzyme produced in a bioreactor on a wheat bran medium has been successfully immobilized with a view to developing a commercially usable technology for lactose hydrolysis in the food industry. Three chemical and three physical methods of cell disruption were tested and a method of grinding with river sand was found to give highest enzyme activity (720 U). The enzyme was covalently immobilized on gelatin. Immobilized enzyme had optimum pH and temperature of 7.0 and 40 degrees C, respectively and was found to give 49% hydrolysis of lactose in milk after 4 h of incubation. The immobilized enzyme was used for eight hydrolysis batches without appreciable loss in activity. The retention of high catalytic activity compared with the losses experienced with several previously reported immobilized versions of the enzyme is significant. The method of immobilization is simple, effective, and can be used for the immobilization of other enzymes.
|
19198767
|
10.1007/s12010-009-8542-y
|
Munish Puri; Shivani Gupta; Parveen Pahuja; Aneet Kaur; J R Kanwar; J F Kennedy
|
Applied biochemistry and biotechnology
|
2010
|
2025-11-10T15:53:40.979316
|
|||||||
21473267
|
pubmed_async
|
literature
|
Ethanol production from whey permeate in a continuous anaerobic bioreactor by Kluyveromyces marxianus.
|
The possibility of using whey permeate as a raw material for the production of ethanol by continuous fermentation by using Kluyveromyces marxianus was examined. The ethanol formation was investigated as a function of the hydraulic retention time in a UASB reactor. The initial lactose concentration in permeate was 50.0 g L(-1). The hydraulic retention time supplied were 12, 24, 48 h and the daily ethanol formations were 4.46, 8.61, 7.73 g L(-1), respectively. The yield coefficient of ethanol was 0.089 g ethanol g(-1) lactose when the hydraulic retention time was 12 h and raised to 0.325 g g(-1) when the hydraulic retention time was as long as 48 h. The results indicated that in ethanol fermentation the hydraulic retention time should be 24 h to obtain high rates of ethanol formation and to avoid product inhibition.
|
21473267
|
10.1080/09593331003616805
|
M Jedrzejewska; K Kozak
|
Environmental technology
|
2011
|
2025-11-10T15:53:40.979557
|
|||||||
31307575
|
pubmed_async
|
literature
|
High-temperature ethanol production by a series of recombinant xylose-fermenting Kluyveromyces marxianus strains.
|
Thermotolerant yeast Kluyveromyces marxianus can assimilate xylose but cannot produce ethanol from xylose under anaerobic conditions. Here, we constructed two recombinant K. marxianus strains, DMB5 and DMB13, that express xylose reductase (XR), NAD
|
31307575
|
10.1016/j.enzmictec.2019.109359
|
Toshihiro Suzuki; Tamotsu Hoshino; Akinori Matsushika
|
Enzyme and microbial technology
|
2019
|
2025-11-10T15:53:40.979848
|
|||||||
14689315
|
pubmed_async
|
literature
|
Evaluation of Kluyveromyces marxianus FII 510700 grown on a lactose-based medium as a source of a natural bioemulsifier.
|
Mannoprotein with emulsification properties was extracted from the cell walls of Kluyveromyces marxianus grown on a lactose-based medium by autoclaving cells in a citrate buffer at pH 7. The purified product was evaluated for chemical and physical stability to establish its potential use as a natural emulsifier in processed foods. The yield of purified bioemulsifier from this strain of K. marxianus was 4-7% of the original dry cell weight. The purified product, at a concentration of 12 g l(-1), formed emulsions that were stable for 3 months when subjected to a range of pH (3-11) and NaCl concentrations (2-50 g l(-1)). The composition of this mannoprotein was 90% carbohydrate (mannan) and 4-6% protein. These values are similar to mannoprotein extracted from cells of Saccharomyces cerevisiae, which is the traditional source. Consequently K. marxianus cultivated on a low-cost lactose-based medium such as whey, a lactose-rich clean waste of the dairy industry, could be developed as a source of bioemulsifier for use in the food industry.
|
14689315
|
10.1007/s10295-003-0105-6
|
Tredwell Lukondeh; Nicholas J Ashbolt; Peter L Rogers
|
Journal of industrial microbiology & biotechnology
|
2003
|
2025-11-10T15:53:40.980114
|
|||||||
11589559
|
pubmed_async
|
literature
|
Yeasts from Water Buffalo Mozzarella, a traditional cheese of the Mediterranean area.
|
Countries of the Mediterranean area are characterized by production of artisanal cheeses, obtained from goat, sheep, cow and buffalo raw milk. The numbers and species of yeasts in the different cheeses are variable, but some species are more frequently detected than others. Kluyveromyces marxianus, K. lactis with their anamorph, Candida kefir, Debaryomyces hansenii and C. famata, C. colliculosa and C. catenulata are dominant species in several cheeses. However, Saccharomyces cerevisiae is often detected in pasta filata cheeses, such as Water Buffalo Mozzarella (WBM) or Cacio Cavallo Podolico. Recently, a comprehensive study of yeasts isolated from Mozzarella cheese produced in Basilicata (Southern Italy) has been carried out. The study has focused on lactose and/or galactose fermenting species (Kluyveromyces and Saccharomyces) to evaluate their role on the functional and sensory properties of the product. End products in milk were evaluated and the biodiversity in terms of production of sulphur dioxide, higher alcohols, ethyl acetate, and acetaldehyde was studied. In particular, S. cerevisiae strains from Water Buffalo Mozzarella cheese, compared to strains isolated from different habitats, such as wine, exhibited considerable difference in the production of some volatile compounds. The diversity observed could be related to the particular microhabitat of S. cerevisiae occurring in whey cheese of water buffalo milk.
|
11589559
|
10.1016/s0168-1605(01)00571-2
|
P Romano; A Ricciardi; G Salzano; G Suzzi
|
International journal of food microbiology
|
2001
|
2025-11-10T15:53:40.980363
|
|||||||
21533904
|
pubmed_async
|
literature
|
Epidemiology and microbiology of nosocomial pediatric candidemia at a northern Indian tertiary care hospital.
|
The availability and aggressive use of chemotherapeutic and immunosuppressive agents as well as broad-spectrum antibacterial agents have created a large population of patients who are at increased risk of acquiring infections with fungal organisms, especially Candida species. Present work was undertaken to study the epidemiology and microbiology of candidemia and Candida colonization in hospitalized children. A total of 323 suspected cases of septicemia were enrolled, of which blood culture from 7.4% subjects was positive for Candida species. In total, 57.3% subjects were colonized by Candida species at least at one of the tested sites. Of 337 isolates, 24.3, 71.5, 2.9, 0.59, and 0.59% were Candida albicans, Candida tropicalis, Candida krusei, Candida kefyr, and Candida lusitaniae, respectively. Antifungal susceptibility results show that fluconazole, itraconazole, and amphotericin B resistance is prevalent in 18.2, 2.4, and 3.6% of C. albicans isolates, and 21.1, 4.6, and 0.04% of C. tropicalis isolates, respectively. In a large number of cases, source of blood infection was patient's own colonizers, as shown by genetic matching. It was also noted that some strain types are circulating within the ward. High prevalence of non-albicans candidemia with high resistance to fluconazole is prevalent in North Indian hospitalized children.
|
21533904
|
10.1007/s11046-011-9431-9
|
Avijit Kumar Awasthi; Amita Jain; Shally Awasthi; Ankur Ambast; Kamlesh Singh; Vijendra Mishra
|
Mycopathologia
|
2011
|
2025-11-10T15:53:40.980665
|
|||||||
11057691
|
pubmed_async
|
literature
|
Yeasts as a model for assessing the toxicity of the fungicides Penconazol, Cymoxanil and Dichlofluanid.
|
In the present work the sensitivity of yeast strains of Kluyveromyces marxianus, Pichia anomala, Candida utilis, Schizosaccharomyces pombe and Saccharomyces cerevisiae, to the fungicides cymoxanil, penconazol, and dichlofluanid, was evaluated. Dichlofluanid induced the most negative effects, whereas penconazol in general was not very toxic. Overall, our results show that the parameters IC50 for specific respiration rates of C. utilis and S. cerevisiae and C(D) for cell viability of S. cerevisiae can be applied to quantify the toxicity level of the above compounds in yeast. Hence, could be explored as an alternative or at least as a complementary test in toxicity studies and, therefore, its potential for inclusion in a tier testing toxicity test battery merits further research.
|
11057691
|
10.1016/s0045-6535(00)00039-4
|
I C Ribeiro; I Veríssimo; L Moniz; H Cardoso; M J Sousa; A M Soares; C Leão
|
Chemosphere
|
2000
|
2025-11-10T15:53:40.980936
|
|||||||
38772384
|
pubmed_async
|
literature
|
Detoxification of Mycotoxin Patulin by the Yeast
|
Patulin (PAT) is a mycotoxin produced by
|
38772384
|
10.1021/acs.jafc.4c02963
|
Mengge Ning; Peng Guo; Jianrui Qi; Yuanyuan Cui; Kai Wang; Gengan Du; Zhouli Wang; Yahong Yuan; Tianli Yue
|
Journal of agricultural and food chemistry
|
2024
|
2025-11-10T15:53:40.981218
|
|||||||
25059876
|
pubmed_async
|
literature
|
Draft Genome Sequence of Kluyveromyces marxianus Strain DMB1, Isolated from Sugarcane Bagasse Hydrolysate.
|
We determined the genome sequence of a thermotolerant yeast, Kluyveromyces marxianus strain DMB1, isolated from sugarcane bagasse hydrolysate, and the sequence provides further insights into the genomic differences between this strain and other reported K. marxianus strains. The genome described here is composed of 11,165,408 bases and has 4,943 protein-coding genes.
|
25059876
|
10.1093/nar/25.5.0955
|
Toshihiro Suzuki; Tamotsu Hoshino; Akinori Matsushika
|
Genome announcements
|
2014
|
2025-11-10T15:53:40.981471
|
|||||||
33457333
|
pubmed_async
|
literature
|
Identification of Candida Species and Antifungal Susceptibility in Cancer Patients with Oral Lesions in Ahvaz, Southern West of Iran.
|
Oral candidiasis is a common disease in cancer patients subject to chemotherapy. The aim of this study was to evaluate the risk factors of rising oral candidiasis incidence and to identify the Candida species isolated from oral lesions of cancer patients and their antifungal sensitivity. A total of 645 patients with cancer were examined. Several A total of 74 isolates of Finally, in addition to emphasis on topical nystatin application in the first stage of oral candidiasis in these patients, using alternative systemic drugs such as fluconazole and amphotericin B can be considered for the resistant candida isolates to nystatin.
|
33457333
|
10.4103/abr.abr_214_19
|
Mehrnoush Maheronnaghsh; Mahnaz Fatahinia; Parvin Dehghan; Ali Teimoori
|
Advanced biomedical research
|
2020
|
2025-11-10T15:53:40.981744
|
|||||||
11866844
|
pubmed_async
|
literature
|
Routine use of CHROMagar Candida medium for presumptive identification of Candida yeast species and detection of mixed fungal populations.
|
OBJECTIVE: To assess the value of the new differential culture medium CHROMagar Candida for routine investigation of clinical specimens. METHODS: During a whole year, 6150 clinical samples were plated on CHROMagar Candida medium. After incubation, the green colonies were considered to be Candida albicans. The colonies of other colors were identified using Bichrolatex-krusei, or by their assimilation pattern on ID 32C test strips and their morphology on rice cream-agar-Tween. RESULTS: Among the 6150 clinical samples, 1643 were positive for fungi. Aspergillus fumigatus and Geotrichum sp. were the predominant filamentous fungi isolated. Candida albicans was the most common species isolated (1274 of the positive samples; 77.5%), and Candida glabrata was the second most common yeast isolated (174 positive samples; 10.6%). Other yeast species were detected at lower frequencies, mainly Candida tropicalis (3.8%), Candida krusei (2.7%), Saccharomyces cerevisiae (2.7%) and Candida kefyr (2.3%), and 16 samples revealed a lipophilic species, Malassezia furfur. Mixed fungal populations accounted for 14.7% of the positive samples. Two or more yeast species were detected in 206 of the 242 specimens containing mixed fungal populations, and five yeast species were detected in one sample. Additionally, we did not observe significant differences in the isolation of yeasts or filamentous fungi from the 366 samples simultaneously plated on CHROMagar Candida and Sabouraud dextrose agar. Close agreement between the two culture media was observed for 89.9% of these samples. CONCLUSIONS: CHROMagar Candida medium was shown to be extremely helpful in a routine clinical mycology service, facilitating the detection of mixed cultures of yeasts and allowing direct identification of C. albicans, as well as rapid presumptive identification of the other yeasts: C. glabrata, C. tropicalis, C. krusei and S. cerevisiae. This chromogenic medium thus appears to be suitable as a primary culture medium, particularly for the mycologic surveillance of immunocompromised patients.
|
11866844
|
10.1016/s1198-743x(14)65143-0
|
Jean-Philippe Bouchara; Philippe Declerck; Bernard Cimon; Claire Planchenault; Ludovic de Gentile; Dominique Chabasse
|
Clinical microbiology and infection : the official publication of the European Society of Clinical Microbiology and Infectious Diseases
|
1996
|
2025-11-10T15:53:40.981995
|
|||||||
10388714
|
pubmed_async
|
literature
|
Nisin production by a mixed-culture system consisting of Lactococcus lactis and Kluyveromyces marxianus.
|
To control the pH during antimicrobial peptide (nisin) production by a lactic acid bacterium, Lactococcus lactis subsp. lactis (ATCC11454), a novel method involving neither addition of alkali nor a separation system such as a ceramic membrane filter and electrodialyzer was developed. A mixed culture of L. lactis and Kluyveromyces marxianus, which was isolated from kefir grains, was utilized in the developed system. The interaction between lactate production by L. lactis and its assimilation by K. marxianus was used to control the pH. To utilize the interaction of these microorganisms to maintain high-level production of nisin, the kinetics of growth of, and production of lactate, acetate, and nisin by, L. lactis were investigated. The kinetics of growth of and lactic acid consumption by K. marxianus were also investigated. Because the pH of the medium could be controlled by the lactate consumption of K. marxianus and the specific lactate consumption rate of K. marxianus could be controlled by changing the dissolved oxygen (DO) concentration, a cascade pH controller coupled with DO control was developed. As a result, the pH was kept constant because the lactate level was kept low and nisin accumulated in the medium to a high level compared with that attained using other pH control strategies, such as with processes lacking pH control and those in which pH is controlled by addition of alkali.
|
10388714
|
10.1128/AEM.65.7.3134-3141.1999
|
H Shimizu; T Mizuguchi; E Tanaka; S Shioya
|
Applied and environmental microbiology
|
1999
|
2025-11-10T15:53:40.982277
|
|||||||
22838397
|
pubmed_async
|
literature
|
A novel Kluyveromyces marxianus strain with an inducible flocculation phenotype.
|
Flocculation is a very useful phenotype for industrial yeast strains, since it facilitates cell harvest and represents an easy way of cell immobilization in continuous fermentation processes. The present work represents the first time that an inducible flocculation phenotype has been generated in a non flocculent strain of Kluyveromyces marxianus. This was accomplished by expressing Saccharomyces cerevisiae FLO5 gene in K. marxianus CECT 11769 strain. The FLO 5 gene was placed under the control of an EPG promoter, not repressed by glucose and induced by anoxia. Our experimental approach successfully generated two novel K. marxianus flocculent phenotypes: one inducible and one constitutive. The constitutive phenotype originated from deletions in the FLO5 promoter region, indicating the existence of putative upstream repressor site involved in oxygen regulation of the EPG1 promoter. The novel strains here generated had a unique set of characteristics that provided an advantage, over the wild-type strain, for the industrial co-production of ethanol and polygalacturonase.
|
22838397
|
10.1016/j.biotechadv.2009.06.006
|
Juan A Vallejo; Manuel Serrat; Irasema Pérez-Portuondo; Angeles Sánchez-Pérez; Jose M Ageitos; Tomas G Villa
|
AMB Express
|
2012
|
2025-11-10T15:53:40.982560
|
|||||||
27564138
|
pubmed_async
|
literature
|
Antifungal properties of hypericin, hypericin tetrasulphonic acid and fagopyrin on pathogenic fungi and spoilage yeasts.
|
The role of hypericin-mediated photodynamic antimicrobial properties on pathogenic fungi and photodynamic therapy for human cancer cells is known. Antifungal properties of Hypericum perforatum L. (Hypericaceae) and Fagopyrum esculentum Moench. (Polygonaceae) extracts were also studied. The different polarities of solvents can cause complication in the identification of antifungal effects of separate biologically active compounds. In recent experimental work, we compared antifungal properties of purified hypericin, hypericin tetrasulphonic acid (hypericin + S) and fagopyrin, which is analogue of hypericin. The antifungal properties of aromatic polyketide derivatives such as hypericin, hypericin + S and fagopyrin on the selected pathogenic fungi and spoilage yeasts have been studied. The antifungal properties of hypericin, hypericin + S and fagopyrin were determined using the broth microdilution method against a set of pathogenic fungi and spoilage yeasts including: Microsporum canis, Trichophyton rubrum, Fusarium oxysporum, Exophiala dermatitidis, Candida albicans, Kluyveromyces marxianus, Pichia fermentans and Saccharomyces cerevisiae. The tested concentrations of hypericin, hypericin + S and fagopyrin ranged from 750 to 0.011 μg/mL and MIC values were evaluated after 48 h incubation at 30 °C. The results confirm different antifungal properties of hypericin, hypericin + S and fagopyrin on the selected pathogenic fungi and spoilage yeasts. For pathogenic fungi, the minimum inhibitory concentrations of hypericin ranged 0.18-46.9 μg/mL, hypericin + S 0.18-750 μg/mL and fagopyrin 11.7-46.9 μg/mL. For spoilage yeasts, the MICs of hypericin and hypericin + S ranged 0.18-46.9 and 0.011-0.73 μg/mL, respectively. The results obtained herein indicate that various chemical structures of hypericin, hypericin + S and fagopyrin can develop different antifungal properties.
|
27564138
|
10.1080/13880209.2016.1211716
|
Oksana Sytar; Jurgita Švedienė; Kristina Ložienė; Algimantas Paškevičius; Anatolij Kosyan; Natalija Taran
|
Pharmaceutical biology
|
2016
|
2025-11-10T15:53:40.982930
|
|||||||
10390820
|
pubmed_async
|
literature
|
Construction of a flocculent Saccharomyces cerevisiae fermenting lactose.
|
A flocculent Saccharomyces cerevisiae strain with the ability to express both the LAC4 (coding for beta-galactosidase) and LAC12 (coding for lactose permease) genes of Kluyveromyces marxianus was constructed. This recombinant strain is not only able to grow on lactose, but it can also ferment this substrate. To our knowledge this is the first time that a recombinant S. cervisiae has been found to ferment lactose in a way comparable to that of the existing lactose-fermenting yeast strains. Moreover, the flocculating capacity of the strain used in this work gives the process several advantages. On the one hand, it allows for operation in a continuous mode at high cell concentration, thus increasing the system's overall productivity; on the other hand, the biomass concentration in the effluent is reduced, thus decreasing product separation/purification costs.
|
10390820
|
10.1007/s002530051441
|
L Domingues; J A Teixeira; N Lima
|
Applied microbiology and biotechnology
|
1999
|
2025-11-10T15:53:40.983227
|
|||||||
21845386
|
pubmed_async
|
literature
|
Investigation of the potential of biocalorimetry as a process analytical technology (PAT) tool for monitoring and control of Crabtree-negative yeast cultures.
|
Biological reaction calorimetry, also known as biocalorimetry, has led to extensive applications in monitoring and control of different bioprocesses. A simple real-time estimator for biomass and growth rate was formulated, based on in-line measured metabolic heat flow values. The performance of the estimator was tested in a unique bench-scale calorimeter (BioRC1), improved to a sensitivity range of 8 mW l(-1) in order to facilitate the monitoring of even weakly exothermic biochemical reactions. A proportional-integral feedback control strategy based on these estimators was designed and implemented to control the growth rate of Candida utilis, Kluyveromyces marxianus and Pichia pastoris by regulating an exponential substrate feed. Maintaining a particular specific growth rate throughout a culture is essential for reproducible product quality in industrial bioprocesses and therefore a key sequence for the step from quality by analysis to quality by design. The potential of biocalorimetry as a reliable biomass monitoring tool and as a key part of a robust control strategy for aerobic fed-batch cultures of Crabtree-negative yeast cells in defined growth medium was investigated. Presenting controller errors of less than 4% in the best cases, the approach paves the way for the development of a generally applicable process analytical technology platform for monitoring and control of microbial fed-batch cultures.
|
21845386
|
10.1007/s00253-011-3507-9
|
Moira Monika Schuler; Senthilkumar Sivaprakasam; Brian Freeland; Adel Hama; Katie-Marie Hughes; Ian W Marison
|
Applied microbiology and biotechnology
|
2012
|
2025-11-10T15:53:41.747429
|
|||||||
29951378
|
pubmed_async
|
literature
|
Phenotypic typing and epidemiological survey of antifungal resistance of
|
Yeast pathogens are emerging agents of nosocomial as well as community-acquired infections and their rapid and accurate identification is crucial for a better management of high-risk patients and for an adequate treatment. We performed a retrospective review of 156 yeast isolates collected during a 17 months' period of regular clinical practice at the Microbiology Department of San Camillo Hospital in Treviso, Italy and analyzed by the traditional culture-based method combined with matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS). Out of all the samples collected MALDI-TOF MS was able to characterize with a MT score ≥1.7 (accurate result at species level) 12 different yeast and yeast-like species from 140 samples: Accurate identification of microorganisms and the study of their antifungal susceptibility allow to understand the epidemiology of a particular area, permitting the choice of the most appropriate early antifungal treatment.
|
29951378
|
10.18683/germs.2018.1132
|
Margherita Scapaticci; Andrea Bartolini; Federica Del Chierico; Cristel Accardi; Francesco Di Girolamo; Andrea Masotti; Maurizio Muraca; Lorenza Putignani
|
Germs
|
2018
|
2025-11-10T15:53:41.748196
|
|||||||
33890624
|
pubmed_async
|
literature
|
Identification of novel pentose transporters in Kluyveromyces marxianus using a new screening platform.
|
The capacity of yeasts to assimilate xylose or arabinose is strongly dependent on plasma membrane transport proteins. Because pentoses comprise a substantial proportion of available sugars in lignocellulosic hydrolysates, their utilisation is centrally important for the development of second generation biorefineries. Relatively few native pentose transporters have been studied and there is intense interest in expanding the repertoire. To aid the identification of novel transporters, we developed a screening platform in the native pentose-utilising yeast Kluyveromyces marxianus. This involved the targeted deletion of twelve transporters of the major facilitator superfamily (MFS) and application of a synthetic biology pipeline for rapid testing of candidate pentose transporters. Using this K. marxianus ΔPT platform, we identified several K. marxianus putative xylose or arabinose transporter proteins that recovered a null strain's ability to growth on these pentoses. Four proteins of the HGT-family were able to support growth in media with high or low concentrations of either xylose or arabinose, while six HXT-like proteins displayed growth only at high xylose concentrations, indicating solely low affinity transport activity. The study offers new insights into the evolution of sugar transporters in yeast and expands the set of native pentose transporters for future functional and biotechnological studies.
|
33890624
|
10.1093/nar/gkv342
|
Lorena Donzella; Javier A Varela; Maria João Sousa; John P Morrissey
|
FEMS yeast research
|
2021
|
2025-11-10T15:53:41.748730
|
|||||||
33616699
|
pubmed_async
|
literature
|
Correction to: Transcriptome analysis of the thermotolerant yeast Kluyveromyces marxianus CCT 7735 under ethanol stress.
|
33616699
|
10.1007/s00253-021-11185-2
|
Raphael Hermano Santos Diniz; Juan C Villada; Mariana Caroline Tocantins Alvim; Pedro Marcus Pereira Vidigal; Nívea Moreira Vieira; Mónica Lamas-Maceiras; María Esperanza Cerdán; María-Isabel González-Siso; Petri-Jaan Lahtvee; Wendel Batista da Silveira
|
Applied microbiology and biotechnology
|
2021
|
2025-11-10T15:53:41.749209
|
||||||||
20087170
|
pubmed_async
|
literature
|
Candida kefyr endocarditis in a patient with hypertrophic obstructive cardiomyopathy.
|
We report an uncommon but emerging fungal pathogen, Candida kefyr, as a causative agent of infective endocarditis in a patient with a known history of hypertrophic obstructive cardiomyopathy. A 74-year-old woman with diabetes type II, hypertrophic obstructive cardiomyopathy, presented with gross hematuria and abdominal pain. Computed tomography scan revealed a hemorrhagic mass in the superior pole of the right kidney, with a thrombus extending from the ureter to the bladder. She underwent cryotherapy of the renal mass, together with retrograde ureteral stent placement, developed hypotension and respiratory distress, spiked high-grade fever, and had a new pansystolic murmur over the mitral and aortic areas. Urine and blood culture grew C. kefyr. Transthoracic echocardiogram revealed large mitral valve vegetation with moderate regurgitation. Micafungin was started, patient responded, and fungemia cleared. Repeat echocardiogram showed small vegetation, preserved leaflet mobility and mild regurgitation. Patient received 10 days of micafungin, followed by 6 weeks of fluconazole.
|
20087170
|
10.1097/MAJ.0b013e3181c0d945
|
Teena Chopra; Ashish Bhargava; Sachin Kumar; Anita Chopra; Sorabh Dhar; Luis Afonso; Jack D Sobel
|
The American journal of the medical sciences
|
2010
|
2025-11-10T15:53:41.749615
|
|||||||
12688475
|
pubmed_async
|
literature
|
Utilization of corn silage juice by Klyuveromyces marxianus.
|
Corn silage juice was found to be a favorable substrate for production of fodder yeasts. Kluyveromyces marxianus NRRL Y-610 yielded significantly more cell dry weight than other cultures examined. In shake-flask experiments, the yeast produced over 13 g of cell dry weight per liter of corn silage juice and completely consumed the organic pollutants (lactic acid, acetic acid, and ethanol). The yeast settled rapidly and had a yeast volume index of 21 ml/g. The results indicate that K. marxianus NRRL Y-610 could be used to efficiently remove lactic acid and other organic compounds from corn silage juice with the concomitant production of fodder yeast.
|
12688475
|
10.1016/s0960-8524(02)00170-0
|
Yong D Hang; Edward E Woodams; Lisa E Hang
|
Bioresource technology
|
2003
|
2025-11-10T15:53:41.750007
|
|||||||
8015561
|
pubmed_async
|
literature
|
Evaluation of two commercialized systems for the rapid identification of medically important yeasts.
|
A total of 77 recent clinical isolates of Candida albicans and other medically important yeasts were identified by two different commercial tests, Rapidec albicans (API-bioMérieux) and Fongiscreen 4H (Sanofi Diagnostics Pasteur), and conventional mycological methods. The strains were from 13 different species of yeasts and consisted of strains of 36 C. albicans, three of Candida famata, nine of Candida (Torulopsis) glabrata, five of Candida guilliermondii, two of Candida kefyr, three of Candida krusei, one of Candida lusitaniae, four of Cryptococcus neoformans, five of Candida parapsilosis, six of Candida tropicalis, one of Candida viswanathii, one of Rhodotorula rubra and one of Saccharomyces cerevisiae. According to the reactivity profiles of the isolates, identification was always correct with Fongiscreen 4H and was correct in 97.3% of the strains with Rapidec albicans. The latter test did not identify two C. albicans isolates that were correctly identified by Fongiscreen 4H. Both methods (97.3% correlation) were very useful for identification of C. albicans achieving the aim of their manufacturers. Additionally, Fongiscreen 4H was very useful for the identification of three other species of yeasts: C. glabrata, C. tropicalis and Cr. neoformans. The results of our study indicate that the accuracy of Rapidec albicans and Fongiscreen 4H is similar to that of the conventional methods used in this study for the identification of C. albicans. The same is true of Fongiscreen 4H in the identification of C. glabrata, C. tropicalis and Cr. neoformans. Both tests could be rapid and easy-to-perform tools in the clinical microbiology laboratory, but differences in cost must be taken into account.
|
8015561
|
10.1111/j.1439-0507.1993.tb00771.x
|
G Quindós; V Lipperheide; J Pontón
|
Mycoses
|
1993
|
2025-11-10T15:53:41.750367
|
|||||||
37269405
|
pubmed_async
|
literature
|
Characterization of mating type on aroma production and metabolic properties wild Kluyveromyces marxianus yeasts.
|
Kluyveromyces marxianus yeasts represent a valuable industry alternative due to their biotechnological potential to produce aromatic compounds. 2-phenylethanol and 2-phenylethylacetate are significant aromatic compounds widely used in food and cosmetics due to their pleasant odor. Natural obtention of these compounds increases their value, and because of this, bioprocesses such as de novo synthesis has become of great significance. However, the relationship between aromatic compound production and yeast's genetic diversity has yet to be studied. In the present study, the analysis of the genetic diversity in K. marxianus isolated from the natural fermentation of Agave duranguensis for Mezcal elaboration is presented. The results of strains in a haploid and diploid state added to the direct relationship between the mating type locus MAT with metabolic characteristics are studied. Growth rate, assimilate carbohydrates (glucose, lactose, and chicory inulin), and the production of aromatic compounds such as ethyl acetate, isoamyl acetate, isoamyl alcohol, 2-phenylethyl butyrate and phenylethyl propionate and the diversity in terms of the output of 2-phenylethanol and 2-phenylethylacetate by de novo synthesis were determinate, obtaining maximum concentrations of 51.30 and 60.39 mg/L by ITD0049 and ITD 0136 yeasts respectively.
|
37269405
|
10.1186/s13068-018-1232-7
|
P J Adame-Soto; E T Aréchiga-Carvajal; S M González-Herrera; M R Moreno-Jiménez; O M Rutiaga-Quiñones
|
World journal of microbiology & biotechnology
|
2023
|
2025-11-10T15:53:41.750912
|
|||||||
17233766
|
pubmed_async
|
literature
|
Physiology of the yeast Kluyveromyces marxianus during batch and chemostat cultures with glucose as the sole carbon source.
|
Growth, substrate consumption, metabolite formation, biomass composition and respiratory parameters of Kluyveromyces marxianus ATCC 26548 were determined during aerobic batch and chemostat cultivations, using mineral medium with glucose as the sole carbon source, at 30 degrees C and pH 5.0. Carbon balances closed within 95-101% in all experiments. A maximum specific growth rate of 0.56 h(-1), a biomass yield on glucose of 0.51 g g(-1), and a maximum specific consumption of oxygen of 11.1 mmol g(-1) h(-1) were obtained during batch cultures. The concentration of excreted metabolites was very low at the culture conditions applied, representing 6% of the consumed carbon at most. Acetate and pyruvate were excreted to a larger extent than ethanol under the batch conditions, and the protein content accounted for 54.6% of the biomass dry weight. Steady states were obtained during chemostats at dilution rates of 0.1, 0.25 and 0.5 h(-1). At the two former dilution rates, cells grew at carbon limitation and the biomass yield on glucose was similar to that obtained under the batch conditions. Metabolite formation was rather low, accounting for a total of 0.005 C-mol C-mol(-1) substrate. At 0.5 h(-1), although the biomass yield on glucose was similar to the value obtained under the above-mentioned conditions, the cultivation was not under carbon limitation. Under this condition, 2-oxoglutarate, acetate, pyruvate and ethanol were the prevalent metabolites excreted. Total metabolite formation only accounted to 0.056 C-mol C-mol(-1) of substrate. A very high protein and a low carbohydrate content (71.9% and 9.6% of biomass dry weight, respectively) were measured in cells under this condition. It is concluded that K. marxianus aligns with the so-called aerobic-respiring or Crabtree-negative yeasts. Furthermore, it has one of the highest growth rates among yeasts, and a high capacity of converting sugar into biomass, even when carbon is not the limiting nutrient. These results provide useful data regarding the future application of K. marxianus in processes aimed at the production of biomass-linked compounds, with high yields and productivities.
|
17233766
|
10.1111/j.1567-1364.2006.00192.x
|
Gustavo Graciano Fonseca; Andreas Karoly Gombert; Elmar Heinzle; Christoph Wittmann
|
FEMS yeast research
|
2007
|
2025-11-10T15:53:41.751318
|
|||||||
40375045
|
pubmed_async
|
literature
|
Safety aspects and in vitro probiotic assessment of Kluyveromyces marxianus strains from neonatal faeces.
|
The isolation and identification of probiotic yeasts is increasing rapidly. In this context, the present study aimed to isolate and identify yeast strains from neonatal faeces in Erzurum province, Türkiye and to determine their probiotic characteristics. A total of 12 yeast strains were isolated and genotypic characterization revealed the presence of seven different species, including Kluyveromyces marxianus, Candida spp. Clavispora lusitaniae, Geotrichum candidum, Trichophyton rubrum, Pichia cactophila, and Meyerozyma guilliermondii. The non-pathogenic and potentially probiotic characteristics of the K. marxianus M2, M9, and M10 strains were further investigated. Although yeast has been isolated from neonatal faeces before, K. marxianus was isolated for the first time in this study. The results revealed that the K. marxianus strains exhibited high resistance to simulated gastric juice and bile salts. The auto-aggregation percentages of the strains ranged from 92.55 to 94.78% after 4 h, while the co-aggregation percentages with pathogens ranged from 19.70 to 53.09%. The K. marxianus M2 strain exhibited the highest degree of hydrophobicity (74.97%), and none of the strains demonstrated DN-ase or haemolytic activity. Furthermore, M2 and M9 strains displayed bile salt hydrolase activity. In conclusion, based on in vitro probiotic test results, K. marxianus strains were selected as probiotic yeast candidates for further studies, especially in patients under antibiotic therapy.
|
40375045
|
10.1007/s10068-023-01268-3
|
Hacer Meral-Aktaş; Bülent Çetin; Muhammet Akif Güler; Bülent Albayrak; Kadir Şerafettin Tekgündüz; Mustafa Kara; Ali Işlek
|
Antonie van Leeuwenhoek
|
2025
|
2025-11-10T15:53:41.751889
|
|||||||
18997852
|
pubmed_async
|
literature
|
Comparison of enzymatic antioxidant defence systems in different metabolic types of yeasts.
|
The enzymatic defence system in the 2 yeasts Kluyveromyces marxianus and Rhodotorula glutinis, differing in their mode of oxygen uptake and energy generation, was characterized and compared with the well-studied facultatively fermentative Crabtree-positive Saccharomyces cerevisiae strain. Twofold higher superoxide dismutase (SOD) and catalase activities were detected in K. marxianus and R. glutinis when cells were cultured on glucose. Further increases of 10%-15% in SOD activity and 30%-50% in catalase were measured in all studied yeasts strains after transfer to media containing ethanol. An evaluation of the ratio of Cu/Zn SOD / Mn SOD was performed as a measure of the oxidative metabolism. A 20% decrease was observed when the respiratory source of energy was ethanol, with the lowest ratio being observed for the oxidative type of K. marxianus yeasts. Electrophoretic analysis revealed that all tested strains possess active Cu/Zn and Mn SODs. A reverse electrophoretic mobility pattern of K. marxianus and R. glutinis SOD enzymes was observed in comparison with the same couple in S. cerevisiae. The investigation of electrophoretic profile of catalase enzymes showed that alongside their different taxonomic status and fermentative capacity, all tested strains possess 2 separate catalases. The role of antioxidant enzymes in preventing oxidant-induced cytotoxicity (treatment with hydrogen peroxide, paraquat, and menadione) was shown.
|
18997852
|
10.1139/w08-093
|
Dafinka I Koleva; Ventsislava Y Petrova; Anna V Kujumdzieva
|
Canadian journal of microbiology
|
2008
|
2025-11-10T15:53:41.752291
|
|||||||
38592508
|
pubmed_async
|
literature
|
Transcriptome analysis of Kluyveromyces marxianus under succinic acid stress and development of robust strains.
|
Kluyveromyces marxianus has become an attractive non-conventional yeast cell factory due to its advantageous properties such as high thermal tolerance and rapid growth. Succinic acid (SA) is an important platform molecule that has been applied in various industries such as food, material, cosmetics, and pharmaceuticals. SA bioproduction may be compromised by its toxicity. Besides, metabolite-responsive promoters are known to be important for dynamic control of gene transcription. Therefore, studies on global gene transcription under various SA concentrations are of great importance. Here, comparative transcriptome changes of K. marxianus exposed to various concentrations of SA were analyzed. Enrichment and analysis of gene clusters revealed repression of the tricarboxylic acid cycle and glyoxylate cycle, also activation of the glycolysis pathway and genes related to ergosterol synthesis. Based on the analyses, potential SA-responsive promoters were investigated, among which the promoter strength of IMTCP2 and KLMA_50231 increased 43.4% and 154.7% in response to 15 g/L SA. In addition, overexpression of the transcription factors Gcr1, Upc2, and Ndt80 significantly increased growth under SA stress. Our results benefit understanding SA toxicity mechanisms and the development of robust yeast for organic acid production. KEY POINTS: • Global gene transcription of K. marxianus is changed by succinic acid (SA) • Promoter activities of IMTCP2 and KLMA_50123 are regulated by SA • Overexpression of Gcr1, Upc2, and Ndt80 enhanced SA tolerance.
|
38592508
|
10.1007/s00253-024-13097-3
|
Du-Wen Zeng; Yong-Qiang Yang; Qi Wang; Feng-Li Zhang; Mao-Dong Zhang; Sha Liao; Zhi-Qiang Liu; Ya-Chao Fan; Chen-Guang Liu; Lin Zhang; Xin-Qing Zhao
|
Applied microbiology and biotechnology
|
2024
|
2025-11-10T15:53:41.752837
|
|||||||
39466458
|
pubmed_async
|
literature
|
Heterologous expressing melittin in a probiotic yeast to evaluate its function for promoting NSC-34 regeneration.
|
Melittin is a bioactive peptide and the predominant component in bee venom (BV), studied for its many medical properties, such as antibacterial, anti-inflammatory, anti-arthritis, nerve damage reduction, and muscle cell regeneration. Melittin is primarily obtained through natural extraction and chemical synthesis; however, both methods have limitations and cannot be used for mass production. This study established a heterologous melittin expression system in the probiotic yeast Kluyveromyces marxianus. This yeast was selected for its advantages in stress tolerance and high secreted protein yields, simplifying purification. A > 95% high-purity melittin (MET) and its precursor promelittin (ProMET) were successfully produced and purified at 1.68 μg/mL and 3.33 μg/mL concentrations and verified through HPLC and mass spectrum. The functional test of the NSC-34 cell regeneration revealed that MET achieved the best activity compared to ProMET and the natural-extracted BV groups. Growth-related gene expressions were evaluated, including microtubule-associated protein 2 (MAP2), microtubule-associated protein Tau (MAPT), growth-associated protein 43 (GAP-43), choline acetyltransferase (ChAT), vesicular acetylcholine transporter (VAChT), and acetylcholine esterase (AChE). The results indicated that treating MET increased MAP2, GAP-43, and VAChT expressions, in which cholinergic signaling is related to neurological functions. A heterologously expressed melittin in a probiotic yeast and its potential for promoting NSC-34 regeneration described here facilitate commercial and therapeutic use. KEY POINTS: • MET and its precursor ProMET were successfully hetero-expressed in K. marxianus • > 95% high-purity MET and ProMET were purified at 1.68 μg/mL and 3.33 μg/mL • MET has no cytotoxicity toward NSC-34 and significantly promotes NSC-34 growth.
|
39466458
|
10.1007/s00253-024-13336-7
|
Hsiao-Yun Huang; Hung-Yi Hsu; Cheng-Yu Kuo; Mao-Lun Wu; Chien-Chen Lai; Gary Ro-Lin Chang; Yu-Ju Lin
|
Applied microbiology and biotechnology
|
2024
|
2025-11-10T15:53:41.753405
|
|||||||
20092622
|
pubmed_async
|
literature
|
Heterologous expression of glucose oxidase in the yeast Kluyveromyces marxianus.
|
In spite of its advantageous physiological properties for bioprocess applications, the use of the yeast Kluyveromyces marxianus as a host for heterologous protein production has been very limited, in constrast to its close relative Kluyveromyces lactis. In the present work, the model protein glucose oxidase (GOX) from Aspergillus niger was cloned into K. marxianus CBS 6556 and into K. lactis CBS 2359 using three different expression systems. We aimed at verifying how each expression system would affect protein expression, secretion/localization, post-translational modification, and biochemical properties. The highest GOX expression levels (1552 units of secreted protein per gram dry cell weight) were achieved using an episomal system, in which the INU1 promoter and terminator were used to drive heterologous gene expression, together with the INU1 prepro sequence, which was employed to drive secretion of the enzyme. In all cases, GOX was mainly secreted, remaining either in the periplasmic space or in the culture supernatant. Whereas the use of genetic elements from Saccharomyces cerevisiae to drive heterologous protein expression led to higher expression levels in K. lactis than in K. marxianus, the use of INU1 genetic elements clearly led to the opposite result. The biochemical characterization of GOX confirmed the correct expression of the protein and showed that K. marxianus has a tendency to hyperglycosylate the protein, in a similar way as already observed for other yeasts, although this tendency seems to be smaller than the one of e.g. K. lactis and S. cerevisiae. Hyperglycosylation of GOX does not seem to affect its affinity for the substrate, nor its activity. Taken together, our results indicate that K. marxianus is indeed a good host for the expression of heterologous proteins, not only for its physiological properties, but also because it correctly secretes and folds these proteins.
|
20092622
|
10.1038/227680a0
|
Saul N Rocha; José Abrahão-Neto; María E Cerdán; María I González-Siso; Andreas K Gombert
|
Microbial cell factories
|
2010
|
2025-11-10T15:53:41.753957
|
|||||||
33465474
|
pubmed_async
|
literature
|
Recent advances in systems and synthetic biology approaches for developing novel cell-factories in non-conventional yeasts.
|
Microbial bioproduction of chemicals, proteins, and primary metabolites from cheap carbon sources is currently an advancing area in industrial research. The model yeast, Saccharomyces cerevisiae, is a well-established biorefinery host that has been used extensively for commercial manufacturing of bioethanol from myriad carbon sources. However, its Crabtree-positive nature often limits the use of this organism for the biosynthesis of commercial molecules that do not belong in the fermentative pathway. To avoid extensive strain engineering of S. cerevisiae for the production of metabolites other than ethanol, non-conventional yeasts can be selected as hosts based on their natural capacity to produce desired commodity chemicals. Non-conventional yeasts like Kluyveromyces marxianus, K. lactis, Yarrowia lipolytica, Pichia pastoris, Scheffersomyces stipitis, Hansenula polymorpha, and Rhodotorula toruloides have been considered as potential industrial eukaryotic hosts owing to their desirable phenotypes such as thermotolerance, assimilation of a wide range of carbon sources, as well as ability to secrete high titers of protein and lipid. However, the advanced metabolic engineering efforts in these organisms are still lacking due to the limited availability of systems and synthetic biology methods like in silico models, well-characterised genetic parts, and optimized genome engineering tools. This review provides an insight into the recent advances and challenges of systems and synthetic biology as well as metabolic engineering endeavours towards the commercial usage of non-conventional yeasts. Particularly, the approaches in emerging non-conventional yeasts for the production of enzymes, therapeutic proteins, lipids, and metabolites for commercial applications are extensively discussed here. Various attempts to address current limitations in designing novel cell factories have been highlighted that include the advances in the fields of genome-scale metabolic model reconstruction, flux balance analysis, 'omics'-data integration into models, genome-editing toolkit development, and rewiring of cellular metabolisms for desired chemical production. Additionally, the understanding of metabolic networks using
|
33465474
|
10.1016/j.biotechadv.2021.107695
|
Pradipta Patra; Manali Das; Pritam Kundu; Amit Ghosh
|
Biotechnology advances
|
2021
|
2025-11-10T15:53:41.754379
|
|||||||
20822567
|
pubmed_async
|
literature
|
Effect of freeze-drying on viability and in vitro probiotic properties of a mixture of lactic acid bacteria and yeasts isolated from kefir.
|
The effect of freeze-drying on viability and probiotic properties of a microbial mixture containing selected bacterial and yeast strains isolated from kefir grains (Lactobacillus kefir, Lactobacillus plantarum, Lactococcus lactis, Saccharomyces cerevisiae and Kluyveromyces marxianus) was studied. The microorganisms were selected according to their potentially probiotic properties in vitro already reported. Two types of formulations were performed, a microbial mixture (MM) suspended in milk and a milk product fermented with MM (FMM). To test the effect of storage on viability of microorganisms, MM and FMM were freeze-dried and maintained at 4°C for six months. After 180 days of storage at 4°C, freeze-dried MM showed better survival rates for each strain than freeze-dried FMM. The addition of sugars (trehalose or sucrose) did not improve the survival rates of any of the microorganisms after freeze-drying. Freeze-drying did not affect the capacity of MM to inhibit growth of Shigella sonnei in vitro, since the co-incubation of this pathogen with freeze-dried MM produced a decrease of 2 log in Shigella viability. The safety of freeze-dried MM was tested in mice and non-translocation of microorganisms to liver or spleen was observed in BALB/c mice feed ad libitum during 7 or 20 days. To our knowledge, this is the first report about the effect of freeze-drying on viability, in vitro probiotic properties and microbial translocation of a mixture containing different strains of both bacteria and yeasts isolated from kefir.
|
20822567
|
10.1017/S0022029910000610
|
Patricia A Bolla; María de los Angeles Serradell; Patricio J de Urraza; Graciela L De Antoni
|
The Journal of dairy research
|
2011
|
2025-11-10T15:53:41.754816
|
|||||||
24521080
|
pubmed_async
|
literature
|
A broad-range yeast expression system reveals Arxula adeninivorans expressing a fungal self-sufficient cytochrome P450 monooxygenase as an excellent whole-cell biocatalyst.
|
The feasibility of using a single vector to clone a cytochrome P450 monooxygenase (P450) in different yeasts and then compare whole-cell hydroxylase activity was investigated. A broad-range yeast expression vector using the ylTEFp to drive expression of the cloned gene and the scTEFp to drive the hygromycin resistance marker gene was used to clone the genes encoding two self-sufficient P450s, CYP102A1 and CYP505A1. Both genes were cloned into Saccharomyces cerevisiae, Kluyveromyces marxianus, Yarrowia lipolytica (two strains) and Arxula adeninivorans. 4-Hexylbenzoic acid (HBA), which is subterminally hydroxylated by both CYP102A1 and CYP505A1, was used to compare whole-cell hydroxylase activity of transformants. Kluyveromyces marxianus and A. adeninivorans exhibited activity with both CYP102A1 and CYP505A1, while S. cerevisiae only displayed CYP102A1 activity and Y. lipolytica only CYP505A1 activity. The highest CYP102A1 activity (0.8 mM HBA converted in 24 h) was observed with concentrated resting-cell suspensions of S. cerevisiae. The CYP505A1 activity observed with growing cultures of A. adeninivorans was however at least 12 times higher than the CYP102A1 activity of S. cerevisiae with up to 2 mM HBA converted within 6 h. The use of K. marxianus and A. adeninivorans for P450 expression has not previously been reported.
|
24521080
|
10.1111/1567-1364.12142
|
Chrispian W Theron; Michel Labuschagné; Ramakrishna Gudiminchi; Jacobus Albertyn; Martha S Smit
|
FEMS yeast research
|
2014
|
2025-11-10T15:53:41.755226
|
|||||||
37991101
|
pubmed_async
|
literature
|
Porungo cheese whey: a new substrate to produce β-galactosidase.
|
The bioconversion of porungo cheese whey to produce β-galactosidase in batch system was studied. The whey released after curd cutting and precipitation during porungo cheese production was collected in borosilicate flasks. Two strains of Kluyveromyces marxianus, CCT 4086 and CBS 6556, and whey supplementation with different nitrogen sources were evaluated. Different temperatures (30 °C and 37 °C) and pH values (5.0 to 7.0) were investigated to establish the best conditions for enzyme production. The highest enzymatic activity was obtained by K. marxianus CCT 4086 in porungo cheese whey supplemented with yeast extract (16.73 U mL-1). K. marxianus CCT 4086 produced superior β-galactosidase activity when compared to CBS 6556 for all media tested (ranging from 11.69 to 14.40 U mL-1). Highest β-galactosidase activity was reached under conditions of pH 7.0 and 30 °C using K. marxianus CCT 4086 in the better media composition. The lowest enzymatic activity was observed at 37 °C for all pH values tested (10.69 U mL-1 to 13.94 U mL-1) and a highest β-galactosidase activity was reached in pH 7.0 for both two temperatures (11.42 to 15.93 U mL-1). Porungo cheese whey shows potential for industrial β-galactosidase production by microbial fermentation.
|
37991101
|
10.1590/0001-3765202320200483
|
Rafaela J S Coelho; Sabrina Gabardo; Aline Vitória C Marim; Lais S Bolognesi; Natan J Pimentel Filho; Marco Antônio Z Ayub
|
Anais da Academia Brasileira de Ciencias
|
2023
|
2025-11-10T15:53:41.755680
|
|||||||
33860834
|
pubmed_async
|
literature
|
A biorefinery concept for the production of fuel ethanol, probiotic yeast, and whey protein from a by-product of the cheese industry.
|
Agroindustrial by-products and residues can be transformed into valuable compounds in biorefineries. Here, we present a new concept: production of fuel ethanol, whey protein, and probiotic yeast from cheese whey. An initial screening under industrially relevant conditions, involving thirty Kluyveromyces marxianus strains, was carried out using spot assays to evaluate their capacity to grow on cheese whey or on whey permeate (100 g lactose/L), under aerobic or anaerobic conditions, in the absence or presence of 5% ethanol, at pH 5.8 or pH 2.5. The four best growing K. marxianus strains were selected and further evaluated in a miniaturized industrial fermentation process using reconstituted whey permeate (100 g lactose/L) with cell recycling (involving sulfuric acid treatment). After five consecutive fermentation cycles, the ethanol yield on sugar reached 90% of the theoretical maximum in the best cases, with 90% cell viability. Cells harvested at this point displayed probiotic properties such as the capacity to survive the passage through the gastrointestinal tract and capacity to modulate the innate immune response of intestinal epithelium, both in vitro. Furthermore, the CIDCA 9121 strain was able to protect against histopathological damage in an animal model of acute colitis. Our findings demonstrate that K. marxianus CIDCA 9121 is capable of efficiently fermenting the lactose present in whey permeate to ethanol and that the remaining yeast biomass has probiotic properties, enabling an integrated process for the obtainment of whey protein (WP), fuel ethanol, and probiotics from cheese whey.Key points• K. marxianus-selected strains ferment whey permeate with 90% ethanol yield.• Industrial fermentation conditions do not affect selected yeast probiotic capacity.• Whey permeate, fuel ethanol, and probiotic biomass can be obtained in a biorefinery.
|
33860834
|
10.1007/s00253-020-10408-2
|
María Dolores Pendón; José V Madeira; David E Romanin; Martín Rumbo; Andreas K Gombert; Graciela L Garrote
|
Applied microbiology and biotechnology
|
2021
|
2025-11-10T15:53:41.756159
|
|||||||
7613734
|
pubmed_async
|
literature
|
Effect of T-2 toxin and verrucarin A in combination on Kluyveromyces marxianus.
|
The growth inhibitory effects of combinations of T-2 toxin and verrucarin A on the yeast Kluyveromyces marxianus was studied. A combination index value was derived to indicate the type of interaction that existed between the binary mixture of these two toxins at various ratios and the target yeast cells. The type of interaction was dependent on the ratio of the toxins used to attain a particular level of growth inhibition. Further, the least change in the type and intensity of interaction or the maximally quiescent ratio (MQR) was found to be unique to the growth medium. In a rich medium, the MQR was 1.0 microgram/ml T-2 toxin:0.75 microgram/ml verrucarin A, where the two toxins had a very stable synergistic interaction over a 2 or 3 log value concentration range. Decreasing the nutrients changed the MQR to 1.0 micrograms/ml T-2 toxin:0.38 microgram/ml verrucarin A. Halving the concentration of the cells in the assay changed the MQR to 1.0 microgram/ml T-2 toxin:6.0 micrograms/ml verrucarin A. We have previously shown that the hierarchy of trichothecene toxicity in yeast bioassay is verrucarin A > roridin A > T-2 toxin > diacetoxyscirpenol > HT-2 toxin. The MQR of these toxins in combination with T-2 toxin follows the same order. This study shows an exception to the above order in that verrucarin A and roridin A exchange places.
|
7613734
|
10.1002/nt.2620030208
|
T J Jones; H A Koshinsky; G G Khachatourians
|
Natural toxins
|
1995
|
2025-11-10T15:53:41.756564
|
|||||||
30771532
|
pubmed_async
|
literature
|
Isavuconazole MIC distribution of 29 yeast species responsible for invasive infections (2015-2017).
|
Isavuconazole is a recent extended-spectrum triazole with activity against yeasts. However, few data are available about the in vitro activity of rare yeast species. We report the MIC distribution of isavuconazole compared with fluconazole for a large collection of common or rare yeasts. Isavuconazole and fluconazole MICs were determined using the EUCAST method for 1457 clinical isolates, mainly recovered from invasive infections, belonging to 29 species. They were sent to the National Reference Centre for Invasive Mycoses & Antifungals between January 2015 and October 2017 and species identification was performed using a polyphasic approach (matrix-assisted laser desorption/ionization time of flight analysis and a molecular method). Isavuconazole had effective in vitro activity against Cryptococcus neoformans (MIC90 < 0.25 mg/L), the five most common Candida spp. (MIC90 ≤ 0.5 mg/L for Candida albicans, Candida glabrata, Candida tropicalis, Candida parapsilosis, and Candida krusei) and also against the majority of rare species, including Candida kefyr and Candida lusitaniae. A few isolates of C. albicans (0.7%, 3/404), C. glabrata (2.7%, 5/184), C. tropicalis (1.0%, 1/96) and C. parapsilosis (0.8%, 1/127) exhibited MIC ≥4 mg/L. All were also resistant to fluconazole according to the EUCAST breakpoints. Some isolates with isavuconazole MIC ≥4 mg/L were also observed among rarer species: Meyerozyma guilliermondii (8.7%, 2/23), Wickerhamomyces anomalus (10.0%, 1/10). Other rare species Saprochaete clavata, Magnusiomyces capitatus, and Rhodotorula mucilaginosa had high MIC50 (≥1 mg/L) and MIC90 (≥4 mg/L) and could be considered as resistant to isavuconazole. We confirmed the good in vitro activity of isavuconazole against common Candida, Cryptococcus species and the majority of the rare yeast species studied.
|
30771532
|
10.1016/j.cmi.2019.02.007
|
M Desnos-Ollivier; S Bretagne; A Boullié; C Gautier; F Dromer; O Lortholary
|
Clinical microbiology and infection : the official publication of the European Society of Clinical Microbiology and Infectious Diseases
|
2019
|
2025-11-10T15:53:41.757026
|
|||||||
35455001
|
pubmed_async
|
literature
|
Considering Strain Variation and Non-Type Strains for Yeast Metabolic Engineering Applications.
|
A variety of yeast species have been considered ideal hosts for metabolic engineering to produce value-added chemicals, including the model organism
|
35455001
|
10.1016/j.jbiosc.2021.12.017
|
Xiunan Yi; Hal S Alper
|
Life (Basel, Switzerland)
|
2022
|
2025-11-10T15:53:41.757550
|
|||||||
23329062
|
pubmed_async
|
literature
|
Performance evaluation of Pichia kluyveri, Kluyveromyces marxianus and Saccharomyces cerevisiae in industrial tequila fermentation.
|
Traditionally, industrial tequila production has used spontaneous fermentation or Saccharomyces cerevisiae yeast strains. Despite the potential of non-Saccharomyces strains for alcoholic fermentation, few studies have been performed at industrial level with these yeasts. Therefore, in this work, Agave tequilana juice was fermented at an industrial level using two non-Saccharomyces yeasts (Pichia kluyveri and Kluyveromyces marxianus) with fermentation efficiency higher than 85 %. Pichia kluyveri (GRO3) was more efficient for alcohol and ethyl lactate production than S. cerevisiae (AR5), while Kluyveromyces marxianus (GRO6) produced more isobutanol and ethyl-acetate than S. cerevisiae (AR5). The level of volatile compounds at the end of fermentation was compared with the tequila standard regulation. All volatile compounds were within the allowed range except for methanol, which was higher for S. cerevisiae (AR5) and K. marxianus (GRO6). The variations in methanol may have been caused by the Agave tequilana used for the tests, since this compound is not synthesized by these yeasts.
|
23329062
|
10.1007/s11274-012-1242-8
|
L Amaya-Delgado; E J Herrera-López; Javier Arrizon; M Arellano-Plaza; A Gschaedler
|
World journal of microbiology & biotechnology
|
2013
|
2025-11-10T15:53:41.757972
|
|||||||
22530086
|
pubmed_async
|
literature
|
Susceptibility of clinical Candida species isolates to antifungal agents by E-test, Southern Iran: A five year study.
|
The incidence of fungal infections in immunocompromised patients, especially by Candida species, has increased in recent years. This study was designed to identify Candida species and determine antifungal susceptibility patterns of 595 yeast strains isolated from various clinical specimens. Identification of the isolates were determined by the API 20 C AUX kit and antifungal susceptibilities of the species to fluconazole, amphotericin B, ketoconazole, itraconazole, voriconazole, and caspofungin were determined by the agar-based E-test method. Candida albicans (48%) was the most frequently isolated species, followed by Candida kruzei (16.1%), Candida glabrata (13.5%), Candida kefyr (7.4%), Candida parapsilosis (4.8%), Candida tropicalis (1.7%) and other species (8.5%). Resistance varies depending on the species and the respective antifungal agents. Comparing the MIC90 for all the strains, the lower MIC90 was observed for caspofungin (0.5 µg/ml). The MIC90 for all Candida species were 64 µg/ml for fluconazole, 0.75 µg/ml for amphotericin B, 4 µg/ml for ketoconazole, 4 µg/ml for itraconazole, and 2 µg/ml for voriconazole. Species definition and determination of antifungal susceptibility patterns are advised for the proper management and treatment of patients at risk for systemic candidiasis. Resistance to antifungal agents is an alarming sign for the emerging common nosocomial fungal infections.
|
22530086
|
P Badiee; A Alborzi
|
Iranian journal of microbiology
|
2011
|
2025-11-10T15:53:41.758379
|
||||||||
28955646
|
pubmed_async
|
literature
|
Physiological growth and galactose utilization by dairy yeast
|
The dairy yeast
|
28955646
|
10.1016/j.biortech.2015.11.015
|
Arun Beniwal; Priyanka Saini; Anusha Kokkiligadda; Shilpa Vij
|
3 Biotech
|
2017
|
2025-11-10T15:53:41.758834
|
|||||||
26950757
|
pubmed_async
|
literature
|
Evaluation of hyper thermal acid hydrolysis of Kappaphycus alvarezii for enhanced bioethanol production.
|
Hyper thermal (HT) acid hydrolysis of Kappaphycus alvarezii, a red seaweed, was optimized to 12% (w/v) seaweed slurry content, 180mM H2SO4 at 140°C for 5min. The maximum monosaccharide concentration of 38.3g/L and 66.7% conversion from total fermentable monosaccharides of 57.6g/L with 120gdw/L K. alvarezii slurry were obtained from HT acid hydrolysis and enzymatic saccharification. HT acid hydrolysis at a severity factor of 0.78 efficiently converted the carbohydrates of seaweed to monosaccharides and produced a low concentration of inhibitory compounds. The levels of ethanol production by separate hydrolysis and fermentation with non-adapted and adapted Kluyveromyces marxianus to high concentration of galactose were 6.1g/L with ethanol yield (YEtOH) of 0.19 at 84h and 16.0g/L with YEtOH of 0.42 at 72h, respectively. Development of the HT acid hydrolysis process and adapted yeast could enhance the overall ethanol fermentation yields of K. alvarezii seaweed.
|
26950757
|
10.1016/j.biortech.2016.02.106
|
Chae Hun Ra; Trung Hau Nguyen; Gwi-Taek Jeong; Sung-Koo Kim
|
Bioresource technology
|
2016
|
2025-11-10T15:53:41.759193
|
|||||||
18029043
|
pubmed_async
|
literature
|
Purification and characterization of the enantioselective esterase from Kluyveromyces marxianus CBS 1553.
|
An intracellular esterase from the yeast Kluyveromyces marxianus CBS 1553 with interesting enantioselective hydrolytic activity towards racemic esters of 1,2-O-isopropylidene glycerol (IPG) was purified and characterized. Optimal culture conditions for the obtainment of the enantioselective esterase on a 5 l-fermentation scale were investigated. Two esterase activities (EST1 and EST2) in the crude cell extract were identified by native PAGE with specific activity staining and separated from each other by anion-exchange chromatography. EST1 showed higher activity and enantioselectivity than EST2 in the resolution of racemic IPG acetate and was further purified by hydrophobic interaction chromatography and preparative electrophoresis (final specific activity approximately = 300 U mg(-1), showing a main protein band with a molecular mass of 29 kDa. EST1 showed optimal activity between pH 8.0 and 10.0 and was stable in the pH range 7.0-10.0. Moreover, it was rather thermostable and active up to 80 degrees C, and retained most of its activity in the presence of 15% (v/v) of various organic solvents. The enzyme showed similar Vmax in the hydrolysis of the acetate esters of IPG, whereas the Km value towards (S)-IPG acetate was significantly lower than the one towards the (R)-enantiomer (5.3 and 70 microM, respectively). Finally, comparison of EST1 activity in the presence of different glycerol esters and synthetic substrates with different chain lengths showed a strong preference of this biocatalyst for short-chain substrates.
|
18029043
|
10.1016/j.jbiotec.2007.09.004
|
Daniela Monti; Erica Elisa Ferrandi; Monica Righi; Diego Romano; Francesco Molinari
|
Journal of biotechnology
|
2008
|
2025-11-10T15:53:41.773771
|
|||||||
8483055
|
pubmed_async
|
literature
|
Characterization of two monoclonal antibodies against secretory proteinase of Candida tropicalis DSM 4238.
|
Two murine IgM monoclonal antibodies (mAb; MT1 and MT2), which were produced against the secretory aspartic proteinase of Candida tropicalis DSM 4238, are described. Both antibodies reacted with the native and denatured conformations of the homologous proteinase antigen but showed different patterns of reactivity with other related proteinases (Candida albicans CBS 2730, serotype A; C. albicans ATCC 48867, serotype B; Candida parapsilosis DSM 4237) and with porcine pepsin. Neither of the antibodies inhibited the proteolytic activity of the homologous enzyme. MT1 also reacted with mannoproteins of C. tropicalis DSM 4238 and C. albicans CBS 2730 and immunofluorescence revealed that this antibody bound to the surface of blastoconidia and pseudomycelia of these two Candida species. A reaction with blastoconidia only was observed with C. albicans serotype B. MT1 also reacted weakly with Candida guilliermondii, but not with C. parapsilosis, Candida glabrata, Candida krusei or Candida kefyr. MT2 did not bind to fungal surfaces. Preliminary experiments suggested that mAb MT1 may recognize a carbohydrate epitope, while MT2 binds to an epitope consisting of the protein part of the enzyme. The two antibodies were used in an ELISA for the detection of proteinase antigen. ELISA with MT1 or MT2 as coating antibodies and a specific protein epitope recognizing mAb-biotin conjugate was able to detect 4 ng ml-1 of antigen. Trials with 26 sera from fungemic patients and 14 sera from controls suggest that MT2 is of potential value in antigen-directed serodiagnosis.
|
8483055
|
M Borg-von Zepelin; V Grüness
|
Journal of medical and veterinary mycology : bi-monthly publication of the International Society for Human and Animal Mycology
|
1993
|
2025-11-10T15:53:41.774447
|
||||||||
30473124
|
pubmed_async
|
literature
|
Case Report of a Tubo-ovarian Abscess Caused by Candida kefyr.
|
Candida species are harmless commensals of hosts, including humans, but they can cause infection when the immune system is compromised. Infections with non-albicans species can occur, ranging from urinary tract infections to sepsis, especially among patients in intensive care units. The patient, a 37-year-old woman, presented with severe abdominal pain, fever, and vomiting. The patient's symptoms and fever continued in spite of treatment with antibiotics, and she underwent exploratory laparotomy. Cyst content culture results showed that Candida kefyr was present in the cyst. To the best of our knowledge, this is the first case report of a tubo-ovarian abscess caused by C. kefyr. Rare pathogens can be found in patients with a tubo-ovarian abscess, so culture of the abscess material is important for determining subsequent treatment, particularly in women who require an operation for tubo-ovarian abscess.
|
30473124
|
10.1016/j.jogc.2018.04.025
|
Firat Okmen; Huseyin Ekici; Sabahattin Anil Ari
|
Journal of obstetrics and gynaecology Canada : JOGC = Journal d'obstetrique et gynecologie du Canada : JOGC
|
2018
|
2025-11-10T15:53:41.775459
|
|||||||
20039034
|
pubmed_async
|
literature
|
Saccharomyces cerevisiae CCMI 885 secretes peptides that inhibit the growth of some non-Saccharomyces wine-related strains.
|
The nature of the toxic compounds produced by Saccharomyces cerevisiae CCMI 885 that induce the early death of Hanseniaspora guilliermondii during mixed fermentations, as well as their ability to inhibit the growth of other non-Saccharomyces wine-related strains, was investigated. The killing effect of mixed supernatants towards H. guilliermondii was inactivated by protease treatments, thus revealing the proteinaceous nature of the toxic compounds. Analysis of the protein pattern of mixed supernatants on Tricine SDS-PAGE showed that this S. cerevisiae strain secretes peptides (<10 kDa), which were detected only when death of H. guilliermondii was already established. Death-inducing supernatants were ultrafiltrated by 10 and 2 kDa membranes, respectively, and the inhibitory effect of those permeates were tested in H. guilliermondii cultures. Results indicated that the (2-10) kDa protein fraction of those supernatants seemed to contain antimicrobial peptides active against H. guilliermondii. Thus, the (2-10) kDa protein fraction was concentrated and its inhibitory effect tested against strains of Kluyveromyces marxianus, Kluyveromyces thermotolerans, Torulaspora delbrueckii and H. guilliermondii. Under the growth conditions used for these tests, the (2-10) kDa protein fraction of S. cerevisiae CCMI 885 supernatants exhibited a fungistatic effect against all the strains and a fungicidal effect against K. marxianus.
|
20039034
|
10.1007/s00253-009-2409-6
|
Helena Albergaria; Diana Francisco; Klaus Gori; Nils Arneborg; Francisco Gírio
|
Applied microbiology and biotechnology
|
2010
|
2025-11-10T15:53:41.775970
|
|||||||
33937967
|
pubmed_async
|
literature
|
Uncoupling glucose sensing from GAL metabolism for heterologous lactose fermentation in Saccharomyces cerevisiae.
|
Development of a system for direct lactose to ethanol fermentation provides a market for the massive amounts of underutilized whey permeate made by the dairy industry. For this system, glucose and galactose metabolism were uncoupled in Saccharomyces cerevisiae by deleting two negative regulatory genes, GAL80 and MIG1, and introducing the essential lactose hydrolase LAC4 and lactose transporter LAC12, from the native but inefficient lactose fermenting yeast Kluyveromyces marxianus. Previously, integration of the LAC4 and LAC12 genes into the MIG1 and NTH1 loci was achieved to construct strain AY-51024M. Low rates of lactose conversion led us to generate the Δmig1Δgal80 diploid mutant strain AY-GM from AY-5, which exhibited loss of diauxic growth and glucose repression, subsequently taking up galactose for consumption at a significantly higher rate and yielding higher ethanol concentrations than strain AY-51024M. Similarly, in cheese whey permeate powder solution (CWPS) during three, repeated, batch processes in a 5L bioreactor containing either 100 g/L or 150 g/L lactose, the lactose uptake and ethanol productivity rates were both significantly greater than that of AY-51024M, while the overall fermentation times were considerably lower. Using the Cre-loxp system for deletion of the MIG1 and GAL80 genes to relieve glucose repression, and LAC4 and LAC12 overexpression to increase lactose uptake and conversion provides an efficient basis for yeast fermentation of whey permeate by-product into ethanol.
|
33937967
|
10.1007/s10295-012-1227-5
|
Jing Zou; Xiaohui Chen; Yinghong Hu; Dongguang Xiao; Xuewu Guo; Xuedong Chang; Lisha Zhou
|
Biotechnology letters
|
2021
|
2025-11-10T15:53:41.776493
|
|||||||
23845272
|
pubmed_async
|
literature
|
Simultaneous integration of multiple genes into the Kluyveromyces marxianus chromosome.
|
While Kluyveromyces marxianus is a promising yeast strain for biotechnological applications, genetic engineering of this strain is still challenging, especially when multiple genes are to be transformed. Sequential gene integration, which takes advantage of repetitive insertion/excision of the URA3 gene as a marker, has been the best option until now, because the URA3-deletion mutant is the only precondition for this method. However, we found that the introduced gene is co-excised during the URA3 excision step for next gene introduction, resulting in a very low cumulative probability (<1.57×10⁻⁶ % for 4 genes) of integrating all genes of interest. To overcome this extremely low probability, and to reduce labor and time, all 4 genes were simultaneously transformed. Surprisingly, the infamously high 'non-homologous end joining' activity of K. marxianus enabled simultaneous integration of all 4 genes in a single step, with a probability of 7.9%. Various K. marxianus strains could also be similarly transformed. Our finding not only reduces the labor and time required for such procedures, but also removes a number of preconditions, such as pre-made vectors, selection markers and knockout mutants, which are needed to introduce many genes into K. marxianus.
|
23845272
|
10.1016/j.jbiotec.2013.06.020
|
Paul Heo; Tae-Jun Yang; Soon-Chun Chung; Yuna Cheon; Jun-Seob Kim; Jun-Bum Park; Hyun Min Koo; Kwang Myung Cho; Jin-Ho Seo; Jae Chan Park; Dae-Hyuk Kweon
|
Journal of biotechnology
|
2013
|
2025-11-10T15:53:41.778170
|
|||||||
17118651
|
pubmed_async
|
literature
|
Kinetics of batch ethanol fermentation of cheese-whey powder (CWP) solution as function of substrate and yeast concentrations.
|
A lactose utilizing yeast strain, Kluyveromyces marxianus DSMZ-7239 was used for ethanol formation from cheese-whey powder (CWP) solution in batch experiments. Effects of initial substrate (CWP) and yeast concentrations on the rate and extent of ethanol formation were investigated. The initial pH and oxidation-reduction potential (ORP) was kept at 5 and -250 mV, respectively. The rate and extent of ethanol formation increased with increasing CWP concentration up to 156 g l(-1) (75 g l(-1) sugar) and then decreased for larger CWP concentrations due to substrate inhibition at high sugar concentrations. The ethanol yield coefficient was also maximum (0.54 g EtOH/g sugar) and equal to the theoretical yield at CWP concentration of 156 g l(-1). The growth yield coefficient was found to be Y(x/s)=1.2+/-0.1g biomass g sugar(-1). The rate of sugar utilization and ethanol formation also increased linearly with increasing initial biomass concentrations. A kinetic model describing the rate of sugar utilization and substrate inhibition as function of the initial substrate and the biomass concentrations was developed. The kinetic constants were determined using the experimental data. Model predictions of sugar utilization rates were in good agreement with the experimental data. The results indicated that the initial sugar concentration should be below 75 g l(-1) (CWP<156 g l(-1)) and the initial biomass should be above 850 mg l(-1) to obtain high rates and yields of ethanol formation and to avoid substrate inhibition.
|
17118651
|
10.1016/j.biortech.2006.10.005
|
Serpil Ozmihci; Fikret Kargi
|
Bioresource technology
|
2007
|
2025-11-10T15:53:41.778602
|
|||||||
27282005
|
pubmed_async
|
literature
|
Etiologic Agents and Antifungal Susceptibility of Oral Candidosis from Romanian patients with HIV-infection or type 1 diabetes mellitus.
|
This is the first Romanian investigation of oral candidosis in patients suffering of HIV-infection or type 1 diabetes mellitus (T1DM). Candida albicans was the dominant species in both types of isolates: n = 14 (46.7%) in T1DM, n = 60 (69.8%) in HIV. The most frequent non-albicans Candida spp. were Candida kefyr (n = 6; 20%) in T1DM and Candida dubliniensis (n = 8; 9.3%) in HIV. Resistance to fluconazole was detected only in the HIV non-albicans Candida group (n = 8; 9.3%). All isolates were susceptible to VOR. The experimental drug MXP had MIC values equal or close to the ones of VOR. Echinocandin resistance was more frequent than azole resistance.
|
27282005
|
10.5604/17331331.1197327
|
Bogdan Minea; Valentin Nastasa; Anna Kolecka; Magdalena Mares; Narcisa Marangoci; Irina Rosca; Mariana Pinteala; Monica Hancianu; Mihai Mares
|
Polish journal of microbiology
|
2016
|
2025-11-10T15:53:41.779091
|
|||||||
17650194
|
pubmed_async
|
literature
|
Lactic acid bacteria and yeasts associated with gowé production from sorghum in Bénin.
|
To identify the dominant micro-organisms involved in the production of gowé, a fermented beverage, and to select the most appropriate species for starter culture development. Samples of sorghum gowé produced twice at three different production sites were taken at different fermentation times. DNA amplification by internal transcribed spacer-polymerase chain reaction of 288 lactic acid bacteria (LAB) isolates and 16S rRNA gene sequencing of selected strains revealed that the dominant LAB responsible for gowé fermentation were Lactobacillus fermentum, Weissella confusa, Lactobacillus mucosae, Pediococcus acidilactici, Pediococcus pentosaceus and Weissella kimchii. DNA from 200 strains of yeasts was amplified and the D1/D2 domain of the 26S rRNA gene was sequenced for selected isolates, revealing that the yeasts species were Kluyveromyces marxianus, Pichia anomala, Candida krusei and Candida tropicalis. Gowé processing is characterized by a mixed fermentation dominated by Lact. fermentum, W. confusa and Ped. acidilactici for the LAB and by K. marxianus, P. anomala and C. krusei for the yeasts. The diversity of the LAB and yeasts identified offers new opportunities for technology upgrading and products development in gowé production. The identified species can be used as possible starter for a controlled fermentation of gowé.
|
17650194
|
10.1111/j.1365-2672.2006.03252.x
|
G Vieira-Dalodé; L Jespersen; J Hounhouigan; P L Moller; C M Nago; M Jakobsen
|
Journal of applied microbiology
|
2007
|
2025-11-10T15:53:41.779500
|
|||||||
15291004
|
pubmed_async
|
literature
|
Comparison of volatile compounds produced in model cheese medium deacidified by Debaryomyces hansenii or Kluyveromyces marxianus.
|
The aroma of a deacidified cheese medium is the result of the overall perception of a large number of molecules belonging to different classes. The volatile compound composition of (60%) cheese medium (pH 5.8) deacidified by Debaryomyces hansenii (DCM(Dh)) was compared with the one deacidified by Kluyveromyces marxianus (DCM(Km)). It was determined by dynamic headspace extraction, followed by gas chromatography separation and quantification as well as by mass spectrometry identification. Whatever the media tested, a first class of volatile compounds can be represented by the ones not produced by any of the yeasts, but some of them are affected by K. marxianus or by D. hansenii. A second class of volatile compounds can be represented by the ones produced by K. marxianus, which were essentially esters. Their concentrations were generally higher than their thresholds, explaining the DCM(Km) global fruity odor. A third class can be represented by the ones generated by D. hansenii, which were essentially methyl ketones with fruity, floral (rose), moldy, cheesy, or wine odor plus 2-phenylethanol with a faded-rose odor. The impact of methyl ketones on the DCMDh global flavor was lower than the impact of 2-phenylethanol and even negligible. Therefore, the global faded-rose odor of D. hansenii DCM can be explained by a high concentration of 2-phenylethanol.
|
15291004
|
10.3168/jds.S0022-0302(04)73306-8
|
M N Leclercq-Perlat; G Corrieu; H E Spinnler
|
Journal of dairy science
|
2004
|
2025-11-10T15:53:41.780379
|
|||||||
19966463
|
pubmed_async
|
literature
|
The crucial role of alcohol dehydrogenase Adh3 in Kluyveromyces marxianus mitochondrial metabolism.
|
The function of mitochondrial Adh3 in the thermotolerant yeast Kluyveromyces marxianus was investigated. An ADH3-disrupted mutant exhibited growth retardation on non-fermentable carbon sources, except for ethanol, and this was suppressed by supplementation with antioxidants. Detailed analysis of the phenotype revealed that the mutant showed an increase in the activity of NADH dehydrogenase, sensitivity to H(2)O(2), and accumulation of reactive oxygen species (ROS), and that these carbon sources increased the activity of succinate dehydrogenase. The increase in both activities may reflect enhanced expression of both dehydrogenases by elevation of their substrate levels. The ROS level became low when antioxidants were added. These findings suggest that the ADH3 mutation and such carbon sources cause an elevation of the substrate level of the respiratory chain and eventually of the ROS level via increased expression of primary dehydrogenases, which in turn causes cell growth retardation. Adh3 might thus play a crucial role in the control of the NADH/NAD(+) balance in mitochondria.
|
19966463
|
10.1271/bbb.90609
|
Noppon Lertwattanasakul; Emi Shigemoto; Nadchanok Rodrussamee; Savitree Limtong; Pornthap Thanonkeo; Mamoru Yamada
|
Bioscience, biotechnology, and biochemistry
|
2009
|
2025-11-10T15:53:41.780830
|
|||||||
9063005
|
pubmed_async
|
literature
|
Isolation of yeasts from bovine milk in Belgium.
|
A total of 436 milk samples from non-infected and 80 from infected quarters were investigated: 24.5% of the samples collected from non-infected and 55% of those collected from infected quarters were positive. Normal milk yielded not less than 16 different species and among them many potentially pathogenic yeast species such as C. parapsilosis, C. guilliermondii, C. tropicalis, C. glabrata and T. asahii, all five able to grow at 40 degrees C. In contrast, the yeasts isolated from infected quarters were from 3 species: C. kefyr, C. catenulata and C. lambica, which were also among the yeasts species recovered from normal milk. Among the three species, only one i.e. Candida kefyr is able to grow above 40 degrees C and from there can be considered as potentially pathogenic, even if bacterial association is necessary to cause mastitis.
|
9063005
|
10.1007/BF00436458
|
P E Lagneau; K Lebtahi; D Swinne
|
Mycopathologia
|
1996
|
2025-11-10T15:53:41.781240
|
|||||||
18546449
|
pubmed_async
|
literature
|
Continuous ethanol production from Jerusalem artichoke tubers. I. Use of free cells of Kluyveromyces marxianus.
|
The Continuous fermentation of Jerusalem artichoke juice to ethanol by free cells of Kluyveromyces marxianus UCD (FST) 55-82 has been studied in a continuous-stirred-tank bioreactor at 35 degrees C and pH 4.6. A maximum yield of 90% of the theoretical was obtained at a dilution rate of 0.05 h(-1). About 95% of the sugars were utilized at dilution rates lower than 0.15 h(-1). Volumetric ethanol productivity and volumetric biomass productivity reached maximum values of 7 g ETOH/L/h and 0.6 g dry wt/L/h, respectively, at a dilution rate of 0.2 h(-1). The maintenance energy coefficient for K. marxianus culture was found to be 0.46 g sugar/g biomass/h/ Oscillatory behavior was following a change in dilution rate from a previous steady state and from batch to continuous culture. Values of specific ethanol production rate and specific sugar uptake were found to increase almost linearly with the increase of the dilution rate. The maximum specific ethanol production rate and maximum specific sugar uptake rate were found to be 2.6 g ethanol/g/ cell/h and 7.9 sugars/g cell/h, respectively. Washout occurred at a dilution rate of 0.41 h(-1).
|
18546449
|
10.1002/bit.260240702
|
A Margaritis; P Bajpai
|
Biotechnology and bioengineering
|
1982
|
2025-11-10T15:53:41.781590
|
|||||||
21191616
|
pubmed_async
|
literature
|
Formation of ethyl acetate by Kluyveromyces marxianus on whey: studies of the ester stripping.
|
Kluyveromyces marxianus is capable of converting lactose into ethyl acetate offering a chance for an economical reuse of whey. The microbial formation of ethyl acetate as a bulk product calls for an aerobic process and, thus, the highly volatile ethyl acetate is discharged from the aerated bioreactor. This stripping process was modeled and investigated experimentally. The stripping rate was proportional to the gas flow and nearly independent of the stirring rate since the stripping was governed by the absorption capacity of the exhaust gas rather than the phase transfer. Cooling the exhaust gas did not noticeably influence the stripping. One batch experiment is presented in detail to demonstrate the formation of ethyl acetate by K. maxianus DSM 5422 on whey. Further batch experiments showed that a substantial formation of ethyl acetate only occurred when the yeast growth was limited by a lack of trace elements. The highest product yield observed was 0.25 g ethyl acetate per g lactose which is nearly 50% of the theoretical maximum.
|
21191616
|
10.1007/s00449-010-0504-9
|
Thanet Urit; Christian Löser; Martin Wunderlich; Thomas Bley
|
Bioprocess and biosystems engineering
|
2011
|
2025-11-10T15:53:41.783462
|
|||||||
30210159
|
pubmed_async
|
literature
|
Pathogenic Yeasts Recovered From Acne Vulgaris: Molecular Characterization and Antifungal Susceptibility Pattern.
|
Acne vulgaris is a disorder showing persistent inflammation in the pilosebaceous follicles. It is one of the most prevalent dermatoses that millions of people suffer from globally. The aim of this study was to identify A total of 70 cutaneous samples from acne vulgaris patients suspected to have Overall, 11 C. parapsilosis was the most prevalent
|
30210159
|
10.4103/ijd.IJD_351_17
|
Ayatollah Nasrollahi Omran; Alinaghi Ghiasi Mansori
|
Indian journal of dermatology
|
2018
|
2025-11-10T15:53:41.783910
|
|||||||
32715249
|
pubmed_async
|
literature
|
Isolation of a Novel
|
To overcome the economic losses associated with fungi and their toxic metabolites, environmentally safe and efficient approaches are needed. To this end, biological control using yeasts and safe bacterial strains and their products are being explored to replace synthetic fungicides. In the present study, the biocontrol effect of a yeast strain of
|
32715249
|
10.1016/j.foodcont.2017.08.032
|
Reem Alasmar; Zahoor Ul-Hassan; Randa Zeidan; Roda Al-Thani; Noora Al-Shamary; Hajer Alnaimi; Quirico Migheli; Samir Jaoua
|
ACS omega
|
2020
|
2025-11-10T15:53:41.784385
|
|||||||
29133101
|
pubmed_async
|
literature
|
Antioxidant, antiproliferative, and immunostimulatory effects of cell wall α-d-mannan fractions from Kluyveromyces marxianus.
|
This study evaluated the antioxidant, antiproliferative, and immunostimulatory properties of cell wall α-d-mannan fractions from yeast Kluyveromyces marxianus CCT7735. Filter centrifugation was used to obtain four fractions (KMM-1, KMM-2, KMM-3, and KMM-4) with molecular weight ranging from 7.6 to 75.1kDa. KMM-1 and KMM-2 comprised D-mannose with traces of D-glucose, whereas other fractions contained only D-mannose. Total sugar found in samples ranged from 85.9% to 96.1%, while protein and phenolic contents were 1.21% and 0.41%, respectively. Although only KMM-1 was able to scavenge superoxide radicals, all fractions presented total antioxidant capacity as well as reducing power, hydroxyl-radical scavenging, and copper- and iron-chelating activities. No fraction was cytotoxic to HeLa cells. However, all samples inhibited the proliferation of the tumor cell Hep-G2 and presented minor cytotoxicity against normal 3T3 cells. All fractions showed mitogenic activity in macrophages and all, except KMM-4, induced nitric oxide production in macrophages, suggestive of their immunostimulatory effects.
|
29133101
|
10.1016/j.ijbiomac.2017.11.053
|
Éder Galinari; Jailma Almeida-Lima; Gorete Ribeiro Macedo; Hilário Cuquetto Mantovani; Hugo Alexandre Oliveira Rocha
|
International journal of biological macromolecules
|
2018
|
2025-11-10T15:53:41.785132
|
|||||||
38539885
|
pubmed_async
|
literature
|
Bee Bread: A Promising Source of Bioactive Compounds with Antioxidant Properties-First Report on Some Antimicrobial Features.
|
Bee bread has received attention due to its high nutritional value, especially its phenolic composition, which enhances life quality. The present study aimed to evaluate the chemical and antimicrobial properties of bee bread (BB) samples from Romania. Initially, the bee bread alcoholic extracts (BBEs) were obtained from BB collected and prepared by
|
38539885
|
10.1016/j.ijantimicag.2019.02.015
|
Cornelia-Ioana Ilie; Angela Spoiala; Elisabeta-Irina Geana; Cristina Chircov; Anton Ficai; Lia-Mara Ditu; Eliza Oprea
|
Antioxidants (Basel, Switzerland)
|
2024
|
2025-11-10T15:53:41.785789
|
|||||||
40641152
|
pubmed_async
|
literature
|
PYR1 Biosensor-Driven Genome-Wide CRISPR Screens for Improved Monoterpene Production in
|
Monoterpenes are valued for their roles as flavors, fragrances, insecticides, and energy-dense fuels. Microorganisms provide sustainable biosynthesis routes for these important molecules, but production levels remain limited. Here, we introduce a biosensor-driven microbial engineering strategy to enhance monoterpene production, specifically targeting geraniol. Using mutagenized libraries of the PYR1 receptor─a versatile biosensor from plant ABA signaling pathways with a malleable binding pocket─we screened 24 monoterpenes and identified PYR1 variants responsive to eight, including geraniol. A low background, highly selective geraniol-sensitive PYR1 variant was expressed in the thermotolerant yeast
|
40641152
|
10.1002/bit.28564
|
Nicholas R Robertson; Chase Lenert-Mondou; Alison C Leonard; Aida Tafrishi; Stephanie Carrera; Sangcheon Lee; Yuna Aguilar; Leonardo Sanchez Zamora; Trinity Nguyen; Jesús Beltrán; Mengwan Li; Sean R Cutler; Timothy A Whitehead; Ian Wheeldon
|
ACS synthetic biology
|
2025
|
2025-11-10T15:53:41.786410
|
|||||||
2043279
|
pubmed_async
|
literature
|
Effects of growth temperature on toxicity of T-2 toxin and roridin A to yeast.
|
In yeasts, growth temperature is known to affect the membrane phospholipid content. The effect of temperature on the growth inhibition of Kluyveromyces marxianus and Saccharomyces cerevisiae by the trichothecene mycotoxins, T-2 toxin and roridin A, was investigated. Examination of EC50 values for T-2 toxin and roridin A showed that these toxins were least inhibitory to both yeasts at 30 and 25 degrees C, respectively. Increasing or decreasing growth temperature from these temperatures gradually increased the inhibitory effect of the trichothecene mycotoxins. Temperature may affect the toxicity of the trichothecenes to the yeasts by regulating the composition of yeast cell membranes.
|
2043279
|
T S Gu; H A Koshinsky; G G Khachatourians
|
Biotechnology and applied biochemistry
|
1991
|
2025-11-10T15:53:41.786819
|
||||||||
8662215
|
pubmed_async
|
literature
|
Isolation and characterization of mutants as an approach to a transformation system in Kluyveromyces marxianus.
|
A method to obtain K. marxianus mutants has been developed. Different auxotrophic mutants were isolated by nystatin and snail-enzyme enrichment procedures using an incubation time of 2 h before adding the antibiotic or the enzyme respectively. All his mutants analyzed by complementation tests turned out to belong to the same complementation group. Some of them were transformed and complemented by the S. cerevisiae HIS3 gene. These non-reverting his3 mutants contain no heterologous sequence, which is essential to make them acceptable for application in the food industry.
|
8662215
|
10.1007/s002940050105
|
L Basabe; N Cabrera; V Yong; J Menéndez; J M Delgado; L Rodríguez
|
Current genetics
|
1996
|
2025-11-10T15:53:41.787214
|
|||||||
23142490
|
pubmed_async
|
literature
|
Rapid and accurate identification of isolates of Candida species by melting peak and melting curve analysis of the internally transcribed spacer region 2 fragment (ITS2-MCA).
|
Rapid identification of clinically important yeasts can facilitate the initiation of anti-fungal therapy, since susceptibility is largely species-dependent. We evaluated melting peak and melting curve analysis of the internally transcribed spacer region 2 fragment (ITS2-MCA) as an identification tool for distinguishing between 16 Candida spp., i.e. Candida albicans, Candida bracarensis, Candida dubliniensis, Candida famata, Candida glabrata, Candida guilliermondii, Candida inconspicua, Candida kefyr, Candida krusei, Candida lipolytica, Candida lusitaniae, Candida nivariensis, Candida norvegensis, Candida parapsilosis, Candida tropicalis and Candida sojae, and Saccharomyces cerevisiae and one species pair, i.e. Candida metapsilosis/Candida orthopsilosis. Starting from a cultured isolate, ITS2-MCA led to differentiation of these species within 6 h. According to our findings, ITS2-MCA offers a simple, rapid and cost-effective method for identification of cultured isolates of the clinically most relevant and prevalent Candida species. Further studies will be necessary to evaluate how it performs on mixed samples and clinical samples.
|
23142490
|
10.1016/j.resmic.2012.10.017
|
Ellen Decat; Els Van Mechelen; Bart Saerens; Stefan J T Vermeulen; Teun Boekhout; Steven De Blaiser; Mario Vaneechoutte; Pieter Deschaght
|
Research in microbiology
|
2013
|
2025-11-10T15:53:41.787609
|
|||||||
27026881
|
pubmed_async
|
literature
|
Efficient conversion of xylose to ethanol by stress-tolerant Kluyveromyces marxianus BUNL-21.
|
The fermentation ability of thermotolerant Kluyveromyces marxianus BUNL-21 isolated in Laos was investigated. Comparison with thermotolerant K. marxianus DMKU3-1042 as one of the most thermotolerant yeasts isolated previously revealed that the strain possesses stronger ability for conversion of xylose to ethanol, resistance to 2-deoxyglucose in the case of pentose, and tolerance to various stresses including high temperature and hydrogen peroxide. K. marxianus BUNL-21 was found to have ethanol fermentation activity from xylose that is slightly lower and much higher than that of Scheffersomyces stipitis (Pichia stipitis) at 30 °C and at higher temperatures, respectively. The lower ethanol production seems to be due to large accumulation of acetic acid. The possible mechanism of acetic acid accumulation is discussed. In addition, it was found that both K. marxianus strains produced ethanol in the presence of 10 mM hydroxymethylfurfural or furfural, at a level almost equivalent to that in their absence. Therefore, K. marxianus BUNL-21 is a highly competent yeast for high-temperature ethanol fermentation with lignocellulosic biomass.
|
27026881
|
10.1007/s10295-013-1230-5
|
Sukanya Nitiyon; Chansom Keo-Oudone; Masayuki Murata; Noppon Lertwattanasakul; Savitree Limtong; Tomoyuki Kosaka; Mamoru Yamada
|
SpringerPlus
|
2016
|
2025-11-10T15:53:41.788352
|
|||||||
34945533
|
pubmed_async
|
literature
|
Preparation and Characterization of Pickering Emulsions with Modified Okara Insoluble Dietary Fiber.
|
Modified okara insoluble dietary fiber (OIDF) has attracted great interest as a promising Pickering emulsifier. At present, the modification methods are mainly physicochemical methods, and the research on microbial modified OIDF as stabilizer is not clear. In this work, modified OIDF was prepared by yeast
|
34945533
|
10.1016/j.carbpol.2020.117441
|
Yue Bao; Hanyu Xue; Yang Yue; Xiujuan Wang; Hansong Yu; Chunhong Piao
|
Foods (Basel, Switzerland)
|
2021
|
2025-11-10T15:53:41.788919
|
|||||||
35179639
|
pubmed_async
|
literature
|
Optimization of fermentation for γ-aminobutyric acid (GABA) production by yeast Kluyveromyces marxianus C21 in okara (soybean residue).
|
γ-Aminobutyric acid (GABA) is a non-protein amino acid with a variety of physiological functions. Recently, yeast Kluyveromyces marxianus strains involved in the catabolism and anabolism of GABA can be used as a microbial platform for GABA production. Okara, rich in nutrients, can be used as a low-cost fermentation substrate for the production of functional materials. This study first proved the advantages of the okara medium to produce GABA by K. marxianus C21 when L-glutamate (L-Glu) or monosodium glutamate (MSG) is the substrate. The highest production of GABA was obtained with 4.31 g/L at optimization condition of culture temperature 35 °C, fermentation time 60 h, and initial pH 4.0. Furthermore, adding peptone significantly increased the GABA production while glucose and vitamin B6 had no positive impact on GABA production. This research provided a powerful new strategy of GABA production by K. marxianus C21 fermentation and is expected to be widely utilized in the functional foods industry to increase GABA content for consumers as a daily supplement as suggested.
|
35179639
|
10.1016/j.lwt.2020.110443
|
Lei Zhang; Yang Yue; Xiujuan Wang; Weichang Dai; Chunhong Piao; Hansong Yu
|
Bioprocess and biosystems engineering
|
2022
|
2025-11-10T15:53:41.789454
|
|||||||
26126524
|
pubmed_async
|
literature
|
Looking beyond Saccharomyces: the potential of non-conventional yeast species for desirable traits in bioethanol fermentation.
|
Saccharomyces cerevisiae has been used for millennia in the production of food and beverages and is by far the most studied yeast species. Currently, it is also the most used microorganism in the production of first-generation bioethanol from sugar or starch crops. Second-generation bioethanol, on the other hand, is produced from lignocellulosic feedstocks that are pretreated and hydrolyzed to obtain monomeric sugars, mainly D-glucose, D-xylose and L-arabinose. Recently, S. cerevisiae recombinant strains capable of fermenting pentose sugars have been generated. However, the pretreatment of the biomass results in hydrolysates with high osmolarity and high concentrations of inhibitors. These compounds negatively influence the fermentation process. Therefore, robust strains with high stress tolerance are required. Up to now, more than 2000 yeast species have been described and some of these could provide a solution to these limitations because of their high tolerance to the most predominant stress conditions present in a second-generation bioethanol reactor. In this review, we will summarize what is known about the non-conventional yeast species showing unusual tolerance to these stresses, namely Zygosaccharomyces rouxii (osmotolerance), Kluyveromyces marxianus and Ogataea (Hansenula) polymorpha (thermotolerance), Dekkera bruxellensis (ethanol tolerance), Pichia kudriavzevii (furan derivatives tolerance) and Z. bailii (acetic acid tolerance).
|
26126524
|
10.1093/femsyr/fov053
|
Dorota Radecka; Vaskar Mukherjee; Raquel Quintilla Mateo; Marija Stojiljkovic; María R Foulquié-Moreno; Johan M Thevelein
|
FEMS yeast research
|
2015
|
2025-11-10T15:53:41.789954
|
Subsets and Splits
No community queries yet
The top public SQL queries from the community will appear here once available.