Dataset Viewer
id
string | source
string | category
string | gene_id
string | gene_name
string | gene_symbol
string | protein_id
string | protein_name
string | sequence
string | title
string | abstract
string | full_text
string | pmid
string | doi
string | authors
string | journal
string | year
string | timestamp
string |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
305007909
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geo_async
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expression
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ELOX19-08R2
|
Bioreactor cell culture sample
|
2025-11-10T15:57:19.748227
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||||||||||||
301626081
|
geo_async
|
expression
|
30X-③ (110614_EAS798_lane8)
|
TSS-seq K.marxianus 30X
|
2025-11-10T15:57:19.855413
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||||||||||||
301626078
|
geo_async
|
expression
|
30D-③ (110624_EAS74_lane8)
|
TSS-seq K.marxianus 30D
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2025-11-10T15:57:19.961544
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||||||||||||
100019857
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geo_async
|
expression
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Illumina Genome Analyzer IIx (Kluyveromyces marxianus)
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2025-11-10T15:57:20.068341
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|||||||||||||
305007922
|
geo_async
|
expression
|
ELOX36-08R1
|
Bioreactor cell culture sample
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2025-11-10T15:57:20.175580
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||||||||||||
100020596
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geo_async
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expression
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Illumina MiSeq (Kluyveromyces marxianus)
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2025-11-10T15:57:20.282564
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|||||||||||||
100026472
|
geo_async
|
expression
|
Illumina HiSeq 2500 (Kluyveromyces marxianus)
|
2025-11-10T15:57:20.389026
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|||||||||||||
303713846
|
geo_async
|
expression
|
KMB6
|
KM 6h rep2
|
2025-11-10T15:57:20.494098
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||||||||||||
302897278
|
geo_async
|
expression
|
Kl. marxianus parental gDNA
|
yeast in stationary phase
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2025-11-10T15:57:20.645791
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||||||||||||
307469740
|
geo_async
|
expression
|
Kmarx_46C_BR2
|
DMKU3-1042
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2025-11-10T15:57:20.709288
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||||||||||||
200164344
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geo_async
|
expression
|
Engineering the thermotolerant industrial yeast Kluyveromyces marxianus for anaerobic growth ssing sterol requirements enables anaerobic growth of the thermotolerant yeast Kluyveromyces marxianus
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Analysis of gene expression changes in S. cerevisiae and K. marxianus in response to oxygen-limitation
|
2025-11-10T15:57:20.813427
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||||||||||||
301717187
|
geo_async
|
expression
|
RNA-seq ME rep1
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Kluyveromyces marxianus_mid exponential
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2025-11-10T15:57:20.918463
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||||||||||||
305007912
|
geo_async
|
expression
|
ELOX19-09L3
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Bioreactor cell culture sample
|
2025-11-10T15:57:21.026493
|
||||||||||||
301626085
|
geo_async
|
expression
|
45D-① (110401_lane8)
|
TSS-seq K.marxianus 45D
|
2025-11-10T15:57:21.131481
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||||||||||||
301626084
|
geo_async
|
expression
|
30DS-③ (110614_EAS798_lane2)
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TSS-seq K.marxianus 30DS
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2025-11-10T15:57:21.239244
|
||||||||||||
301626083
|
geo_async
|
expression
|
30DS-② (110614_EAS798_lane1)
|
TSS-seq K.marxianus 30DS
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2025-11-10T15:57:21.344579
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||||||||||||
200070111
|
geo_async
|
expression
|
Transcriptional expression level during exponential growth phase in Kluyveromyces marxianus
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To understand functional roles of ncRNAs during exponential growth phase, we captured transcriptome changes by conducting strand-specific RNA-seq.
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2025-11-10T15:57:21.464154
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||||||||||||
301717189
|
geo_async
|
expression
|
RNA-seq LE rep1
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Kluyveromyces marxianus_late exponential
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2025-11-10T15:57:21.557847
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||||||||||||
301717186
|
geo_async
|
expression
|
RNA-seq EE rep2
|
Kluyveromyces marxianus_early exponential
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2025-11-10T15:57:21.664443
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||||||||||||
301626079
|
geo_async
|
expression
|
30X-① (110614_EAS798_lane6)
|
TSS-seq K.marxianus 30X
|
2025-11-10T15:57:21.772834
|
||||||||||||
303713849
|
geo_async
|
expression
|
KMB36
|
KM 36h rep2
|
2025-11-10T15:57:21.878261
|
||||||||||||
301626077
|
geo_async
|
expression
|
30D-② (110624_EAS74_lane7)
|
TSS-seq K.marxianus 30D
|
2025-11-10T15:57:21.986225
|
||||||||||||
303713847
|
geo_async
|
expression
|
KMC6
|
KM 6h rep3
|
2025-11-10T15:57:22.092435
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||||||||||||
100029573
|
geo_async
|
expression
|
Illumina NovaSeq 6000 (Kluyveromyces marxianus)
|
2025-11-10T15:57:22.200707
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|||||||||||||
200108389
|
geo_async
|
expression
|
Allele-specific mRNA expression experiment in lactis-wickerhamii and lactis-marxianus interespecies hybrids
|
This experiment was performed to measure differences in pathway-level evolution of cis-regulation between closely related Kluyveromyces species. Sequencing of mRNA as well as DNA was performed in hybrids of Kl. lactis with two other species, Kl. wickerhamii and Kl. marxianus. Cells were grown overnight for DNA sequencing and for 4-8h to an OD600=0.7-1.0 for mRNA sequencing. DNA and mRNA reads were mapped to hybrid genomes, multimapping reads discarded, and allele-specific expression ratio for each gene was calculated after first normalizing mRNA reads to the number of DNA reads for each gene in each species. We found that the ribosomal protein genes showed pathway-level allele-specific expression differences between species in both interspecies hybrids.
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2025-11-10T15:57:22.307467
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||||||||||||
301626086
|
geo_async
|
expression
|
45D-② (110614_EAS74_lane7)
|
TSS-seq K.marxianus 45D
|
2025-11-10T15:57:22.412509
|
||||||||||||
100024417
|
geo_async
|
expression
|
Illumina HiSeq 4000 (Kluyveromyces marxianus)
|
2025-11-10T15:57:22.517748
|
|||||||||||||
303713845
|
geo_async
|
expression
|
KMA6
|
KM 6h rep1
|
2025-11-10T15:57:22.622153
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||||||||||||
200066600
|
geo_async
|
expression
|
TSS-seq analysis of K. marxianus
|
Thermotolerant Kluyveromyces marxianus possesses intrinsic abilities to ferment and assimilate a wide variety of substrates including xylose and to efficiently produce proteins. The transcriptome analysis clarified distinctive metabolic pathways under three different growth conditions, static culture, high temperature and xylose medium, in comparison to the control condition of glucose medium under a shaking condition at 30°C. Interestingly, the yeast appears to overcome the issue of reactive oxygen species, which tend to accumulate under all three conditions.
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2025-11-10T15:57:22.729932
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||||||||||||
307469739
|
geo_async
|
expression
|
Kmarx_46C_BR1
|
DMKU3-1042
|
2025-11-10T15:57:22.838118
|
||||||||||||
305007920
|
geo_async
|
expression
|
ELOX34-09L1
|
Bioreactor cell culture sample
|
2025-11-10T15:57:22.944227
|
||||||||||||
303713850
|
geo_async
|
expression
|
KMC36
|
KM 36h rep3
|
2025-11-10T15:57:23.050420
|
||||||||||||
301626087
|
geo_async
|
expression
|
45D-③ (110614_EAS74_lane8)
|
TSS-seq K.marxianus 45D
|
2025-11-10T15:57:23.156053
|
||||||||||||
301717185
|
geo_async
|
expression
|
RNA-seq EE rep1
|
Kluyveromyces marxianus_early exponential
|
2025-11-10T15:57:23.261192
|
||||||||||||
301626082
|
geo_async
|
expression
|
30DS-① (110401_lane7)
|
TSS-seq K.marxianus 30DS
|
2025-11-10T15:57:23.368531
|
||||||||||||
305007910
|
geo_async
|
expression
|
ELOX19-08R3
|
Bioreactor cell culture sample
|
2025-11-10T15:57:23.477498
|
||||||||||||
200234499
|
geo_async
|
expression
|
An adaptive biomolecular condensation response is conserved across environmentally divergent species
|
Cellular responses to maladaptive environmental changes—stresses—allow for organismal adaptation to diverse and dynamic conditions. Across the tree of life, cells upregulate a highly conserved transcriptional program in response to so-called proteotoxic stresses such as heat shock. Correspondingly, in eukaryotes, these stresses induce the formation of biomolecular condensates, clusters of mRNA and protein which are referred to as stress granules under severe stress. However, major questions remain about this stress-induced response. How conserved is the condensation response relative to the transcriptional response? How does it vary across environmental niches, and to what extent does it correspond with the conserved transcriptional response? To answer these fundamental questions, we studied the growth, transcriptional, and condensation heat-induced stress responses in three fungal species adapted to thrive in different thermal environments: cryophilic S. kudriavzevii, mesophilic S. cerevisiae, and thermotolerant K. marxianus. Here we show that transcriptional heat shock responses track each species’ evolved temperature range of growth. Further, orthologous proteins—including poly(A)-binding protein, Pab1, a core marker of stress granules—form condensates in vivo at temperatures systematically tuned to the temperature at which the organisms activate the transcriptional heat shock response and slow their growth. In vitro, purified Pab1 from each species condenses autonomously at niche-specific temperatures. Homologous mutations in Pab1 cause similar shifts in relative condensation temperature across species, and crucially, mutations which suppress condensation in vitro also reduce fitness during heat stress. Our findings indicate that stress-induced protein condensation is adaptive, conserved, integrated with the growth and transcriptional responses, and tuned to features of the cellular and organismal environment to initiate at niche-specific levels.
|
2025-11-10T15:57:23.591853
|
||||||||||||
305007919
|
geo_async
|
expression
|
ELOX34-08L1
|
Bioreactor cell culture sample
|
2025-11-10T15:57:23.687969
|
||||||||||||
305007921
|
geo_async
|
expression
|
ELOX36-08L1
|
Bioreactor cell culture sample
|
2025-11-10T15:57:23.871849
|
||||||||||||
200129483
|
geo_async
|
expression
|
Next Generation Sequencing Facilitates Quantitative Analysis of two wine yeasts: Saccharomyces cerevisiae EC1118 and Kluyveromyces marxianus IWBT Y855
|
K.marxianus and S. cerevisiae differed by their consumption of some amino acids and the production of aroma compounds (especially higher alcohols). To complete this phenotypic observation, RNA sequencing experiment was performed. Two samples were realized: at 6h of fermentation corresponding to lag phase and at the time of half of the maximal population was reached (20h for S. cerevisiae and 36h for K. marxianus). Thanks to this analyse, we can understand what regulations explain the differences between these two strains.
|
2025-11-10T15:57:23.910817
|
||||||||||||
301626076
|
geo_async
|
expression
|
30D-① (110401_lane5)
|
TSS-seq K.marxianus 30D
|
2025-11-10T15:57:24.017836
|
||||||||||||
307469738
|
geo_async
|
expression
|
Kmarx_37C_BR2
|
DMKU3-1042
|
2025-11-10T15:57:24.123331
|
||||||||||||
301717188
|
geo_async
|
expression
|
RNA-seq ME rep2
|
Kluyveromyces marxianus_mid exponential
|
2025-11-10T15:57:24.231232
|
||||||||||||
307469737
|
geo_async
|
expression
|
Kmarx_37C_BR1
|
DMKU3-1042
|
2025-11-10T15:57:24.335979
|
||||||||||||
301626080
|
geo_async
|
expression
|
30X-② (110614_EAS798_lane7)
|
TSS-seq K.marxianus 30X
|
2025-11-10T15:57:24.441626
|
||||||||||||
301717190
|
geo_async
|
expression
|
RNA-seq LE rep2
|
Kluyveromyces marxianus_late exponential
|
2025-11-10T15:57:24.548175
|
||||||||||||
303713848
|
geo_async
|
expression
|
KMA36
|
KM 36h rep1
|
2025-11-10T15:57:24.653953
|
||||||||||||
305007911
|
geo_async
|
expression
|
ELOX19-09L2
|
Bioreactor cell culture sample
|
2025-11-10T15:57:24.764106
|
||||||||||||
23756236
|
pubmed_async
|
literature
|
Yeast dynamics during spontaneous fermentation of mawè and tchoukoutou, two traditional products from Benin.
|
Mawè and tchoukoutou are two traditional fermented foods largely consumed in Benin, West Africa. Their preparations remain as a house art and they are the result of spontaneous fermentation processes. In this study, dynamics of the yeast populations occurring during spontaneous fermentations of mawè and tchoukoutou were investigated using both culture-dependent and -independent approaches. For each product, two productions were followed. Samples were taken at different fermentation times and yeasts were isolated, resulting in the collection of 177 isolates. They were identified by the PCR-DGGE technique followed by the sequencing of the D1/D2 domain of the 26S rRNA gene. The predominant yeast species identified were typed by rep-PCR. Candida krusei was the predominant yeast species in mawè fermentation followed by Candida glabrata and Kluyveromyces marxianus. Other yeast species were detected in lower numbers. The yeast successions that took place during mawè fermentation lead to a final population comprising Saccharomyces cerevisiae, C. krusei and K. marxianus. The yeast populations dominating the fermentation of tchoukoutou were found to consist of S. cerevisiae, almost exclusively. Other yeast species were detected in the early stages of fermentation. For the predominant species a succession of biotypes was demonstrated by rep-PCR for the fermentation of both products. The direct analysis at DNA and RNA levels in the case of mawè did not reveal any other species except those already identified by culture-based analysis. On the other hand, for tchoukoutou, four species were identified that were not detected by the culture-based approach. The spontaneous fermentation of mawè and tchoukoutou in the end were dominated by a few autochthonous species.
|
23756236
|
10.1016/j.ijfoodmicro.2013.05.004
|
Anna Greppi; Kalliopi Rantisou; Wilfrid Padonou; Joseph Hounhouigan; Lene Jespersen; Mogens Jakobsen; Luca Cocolin
|
International journal of food microbiology
|
2013
|
2025-11-10T15:53:40.950757
|
|||||||
38189374
|
pubmed_async
|
literature
|
MALDI-TOF mass spectrometry identification and antifungal susceptibility testing of yeasts causing vulvovaginal candidiasis (VVC) in Tebessa (Northeastern Algeria).
|
Vulvovaginal candidiasis (VVC) alongside with antifungal resistance are becoming a major clinical problem in recent years. A prospective study aimed to evaluate the diversity of yeast strains associated with VVC in Tebessa city (northeastern Algeria) and investigate their susceptibility patterns. Over two months, yeasts were isolated on chromogenic medium from twenty-nine non-pregnant women with symptomatic VVC. The isolates were characterized with MALDI-TOF MS and antifungal susceptibility testing was performed for nine antifungal drugs using SensititreTM YeastOneTM YO10. Twenty-nine non-duplicate yeasts were recovered and the mass spectrometry profiles showed reliable scores of which four genera and five different species were identified. Candida albicans accounted for 65.5 % (n = 19) of the total number of isolates, followed by C. glabrata with 20.7% (n = 6). For the remaining non-albicans Candida (NCA) species, Kluyveromyces marxianus with 6.9% (n = 2), Pichia kudriavzevii and Saccharomyces cerevisiae with one isolate each. The antifungal susceptibilities showed wild type MICs of C. albicans to amphotericin B, azoles and echinocandins. In addition, four C. albicans isolates were resistant to flucytosine. For C. glabrata isolates, 100% non-WT phenotype was found for both posaconazole and itraconazole. For the very first time, the obtained outcomes bring out new data concerning the epidemiology of yeasts causing VVC in Algeria and their antimicrobial susceptibility profiles.
|
38189374
|
10.1684/abc.2023.1852
|
Mabrouka Benhadj; Taha Menasria; Stéphane Ranque
|
Annales de biologie clinique
|
2024
|
2025-11-10T15:53:40.951473
|
|||||||
30806349
|
pubmed_async
|
literature
|
Effect of thermal treatment of whey contaminated with antibiotics on the growth of Kluyveromyces marxianus.
|
The objective of the studies reported in this research communication was to investigate the use of whey contaminated with antibiotics such as cephalosporins, quinolones and tetracyclines as a nutrient medium for the growth of Kluyveromyces marxianus with particular attention to the effect of thermal treatment used to overcome the inhibitory effects of antibiotic concentrations close to the Maximum Residue Limits. The heat treatments at 120 °C for 40 min, 120 °C for 83 min, and 120 °C for 91 min caused total inactivation of cephalosporins, tetracyclines and quinolone residues in whey respectively.
|
30806349
|
10.1017/S0022029919000098
|
Dafna Eluk; Roberto Ceruti; Orlando Nagel; Rafael Althaus
|
The Journal of dairy research
|
2019
|
2025-11-10T15:53:40.951952
|
|||||||
22860591
|
pubmed_async
|
literature
|
Investigation on culturable microflora in Tibetan kefir grains from different areas of China.
|
Four samples of Tibetan kefir grains (TK-ZJUJ 01-04) from Tibet and surrounding areas were investigated via phenotypic and genotypic methods to compare and analyze the diversity of culturable microflora among different origins. As a result, 4 genera of microorganisms from TK-ZJUJ01: Bacillus subtilis (2.9 × 10(7) cfu/mL), Lactococcus lactis (8.2 × 10(7) cfu/mL), Kluyveromyces marxianus (3.0 × 10(6) cfu/mL), Saccharomyces cerevisiae (9.0 × 10(6) cfu/mL); 4 genera from TK-ZJUJ02: Lactobacillus kefiri (1.0 × 10(8) cfu/mL), Pichia kudriavzevii (5.0 × 10(6) cfu/mL), K. marxianus (1.9 × 10(7) cfu/mL), Kazachstania unispora (6.2 × 10(7) cfu/mL); 6 genera from TK-ZJUJ03: Leuconostoc lactis (4.6 × 10(7) cfu/mL), L. lactis (3.0 × 10(7) cfu/mL), Lactobacillus plantarum (3.0 × 10(7) cfu/mL), K. unispora (3.0 × 10(6) cfu/mL), K. marxianus (2.0 × 10(6) cfu/mL), (1.7 × 10(7) cfu/mL); and 4 genera from TK-ZJUJ04: L. plantarum (1.8 × 10(7) cfu/mL), Acetobacter fabarum (5.0 × 10(6) cfu/mL), K. unispora (6.2 × 10(7) cfu/mL), Pichia guilliermondii (6.2 × 10(7) cfu/mL) were identified. Yeasts like P. kudriavzevii and P. guilliermondii isolated in this study were the first time reported in Tibetan kefir grains. For TK-ZJUJ 01-03, lactic acid bacteria were the major microorganisms, which accounted for more than 50% of all the microbial population, while for TK-ZJUJ04, the largest microbial group was yeasts which accounted for more than 50%. In a word, study of diversity and composition of microflora provided us theoretical foundation for further investigation and application of Tibetan kefir grains. This is the basic research in order to develop and industrialize a new kind of yogurt starter which is naturally formed microbiota with both lactic acid bacteria and yeasts in it.
|
22860591
|
10.1111/j.1750-3841.2012.02805.x
|
Jie Gao; Fengying Gu; Nesredin H Abdella; Hui Ruan; Guoqing He
|
Journal of food science
|
2012
|
2025-11-10T15:53:40.952274
|
|||||||
37108722
|
pubmed_async
|
literature
|
Valorisation of Whey Permeate in Sequential Bioprocesses towards Value-Added Products-Optimisation of Biphasic and Classical Batch Cultures of
|
Whey permeate is categorised as hazardous wastewater for aquatic environments, mainly due to its high lactose content. Therefore, it must be valorised before being released into the environment. One pathway for whey permeate management is its use in biotechnological processes. Herein, we present roads for whey permeate valorisation with the
|
37108722
|
10.1016/j.fbp.2016.07.011
|
Karolina Drężek; Maria Krystyna Sobczyk; Zoltán Kállai; Anna Detman; Paula Bardadyn; Jolanta Mierzejewska
|
International journal of molecular sciences
|
2023
|
2025-11-10T15:53:40.952713
|
|||||||
28204651
|
pubmed_async
|
literature
|
Yeasts from Scarlet ibises (Eudocimus ruber): A focus on monitoring the antifungal susceptibility of Candida famata and closely related species.
|
This study aimed to identify yeasts from the gastrointestinal tract of scarlet ibises (Eudocimus ruber) and from plant material collected from the environment where they live. Then, the isolates phenotypically identified as Candida famata were submitted to molecular identification of their closely related species and evaluated for their antifungal susceptibility and possible resistance mechanisms to antifungal drugs. Cloacal swabs from 20 scarlet ibises kept in captivity at Mangal das Garças Park (Brazil), pooled stool samples (n = 20) and samples of trunks and hollow of trees (n = 20) obtained from their enclosures were collected. The samples were seeded on Sabouraud agar supplemented with chloramphenicol. The 48 recovered isolates were phenotypically identified as 15 Candida famata, 13 Candida catenulata, 2 Candida intermedia, 1 Candida lusitaniae, 2 Candida guilliermondii, 1 Candida kefyr, 1 Candida amapae, 1 Candida krusei, 8 Trichosporon spp., and 4 Rhodotorula spp. The C. famata isolates were further identified as 3 C. famata, 8 Debaryomyces nepalensis, and 4 C. palmioleophila. All C. famata and C. palmioleophila were susceptible to caspofungin and itraconazole, while one D. nepalensis was resistant to fluconazole and voriconazole. This same isolate and another D. nepalensis had lower amphotericin B susceptibility. The azole resistant strain had an increased efflux of rhodamine 6G and an alteration in the membrane sterol content, demonstrating multifactorial resistance mechanism. Finally, this research shows that scarlet ibises and their environment harbor C. famata and closely related species, including antifungal resistant isolates, emphasizing the need of monitoring the antifungal susceptibility of these yeast species.
|
28204651
|
10.1093/mmy/myw144
|
Raimunda Sâmia Nogueira Brilhante; Aline Lobão da Silva; Frederico Ozanan Barros Monteiro; Glaucia Morgana de Melo Guedes; Jamille Alencar Sales; Jonathas Sales de Oliveira; José Erisvaldo Maia Junior; Stefânia Araújo Miranda; José Júlio Costa Sidrim; Lucas Pereira de Alencar; Débora Souza Collares Maia Castelo-Branco; Rossana de Aguiar Cordeiro; Waldemiro de Aquino Pereira Neto; Marcos Fábio Gadelha Rocha
|
Medical mycology
|
2017
|
2025-11-10T15:53:40.953097
|
|||||||
28919693
|
pubmed_async
|
literature
|
Antimicrobial activity of yeasts against some pathogenic bacteria.
|
This study was designed to isolate and identify yeast species from milk and meat products, and to test their antimicrobial activity against some bacterial species. A total of 160 milk and meat products samples were collected from random sellers and super markets in New Damietta city, Damietta, Egypt. Samples were subjected to yeast isolation procedures and tested for its antimicrobial activity against The recovery rate of yeasts from sausage was 20% (2/10) followed by kareish cheese, processed cheese, and butter 10% (1/10) each as well as raw milk 9% (9/100), and fruit yoghurt 30% (6/20). Different yeast species were recovered, namely, The obtained results confirmed that some kinds of yeasts have the ability to produce antimicrobial compounds that could inhibit some pathogenic and spoilage bacteria and these antimicrobial activity of yeasts enables them to be one of the novel agents in controlling spoilage of food.
|
28919693
|
10.14202/vetworld.2017.979-983
|
Gamal Younis; Amal Awad; Rehab E Dawod; Nehal E Yousef
|
Veterinary world
|
2017
|
2025-11-10T15:53:40.953489
|
|||||||
24535257
|
pubmed_async
|
literature
|
Physiological characterization of thermotolerant yeast for cellulosic ethanol production.
|
The conversion of lignocellulose into fermentable sugars is considered a promising alternative for increasing ethanol production. Higher fermentation yield has been achieved through the process of simultaneous saccharification and fermentation (SSF). In this study, a comparison was performed between the yeast species Saccharomyces cerevisiae and Kluyveromyces marxianus for their potential use in SSF process. Three strains of S. cerevisiae were evaluated: two are widely used in the Brazilian ethanol industry (CAT-1 and PE-2), and one has been isolated based on its capacity to grow and ferment at 42 °C (LBM-1). In addition, we used thermotolerant strains of K. marxianus. Two strains were obtained from biological collections, ATCC 8554 and CCT 4086, and one strain was isolated based on its fermentative capacity (UFV-3). SSF experiments revealed that S. cerevisiae industrial strains (CAT-1 and PE-2) have the potential to produce cellulosic ethanol once ethanol had presented yields similar to yields from thermotolerant strains. The industrial strains are more tolerant to ethanol and had already been adapted to industrial conditions. Moreover, the study shows that although the K. marxianus strains have fermentative capacities similar to strains of S. cerevisiae, they have low tolerance to ethanol. This characteristic is an important target for enhancing the performance of this yeast in ethanol production.
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24535257
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10.1091/mbc.E10-08-0721
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Daniela A Costa; Carlos J A de Souza; Patrícia S Costa; Marina Q R B Rodrigues; Ancély F dos Santos; Mariana R Lopes; Hugo L A Genier; Wendel B Silveira; Luciano G Fietto
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Applied microbiology and biotechnology
|
2014
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2025-11-10T15:53:40.953893
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40900145
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pubmed_async
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literature
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An update on clinically relevant, rare, and emerging
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SUMMARYMany yeast species causing life-threatening invasive infections that were formerly classified in the genus
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40900145
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10.1128/cmr.00064-23
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Elaine Cristina Francisco; Diego H Caceres; José Guillermo Pereira Brunelli; Guillermo Garcia-Effron; Amir Arastehfar; Felipe de Camargo Ribeiro; Matheus Naves de Almeida; Sarah Santos Gonçalves; João Nóbrega de Almeida; Cornelia Lass-Flörl; Teun Boekhout; Arnaldo Lopes Colombo
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Clinical microbiology reviews
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2025
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2025-11-10T15:53:40.954261
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16346150
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pubmed_async
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literature
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Ethanol Inhibition Kinetics of Kluyveromyces marxianus Grown on Jerusalem Artichoke Juice.
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The kinetics of ethanol inhibition on cell growth and ethanol production by Kluyveromyces marxianus UCD (FST) 55-82 were studied during batch growth. The liquid medium contained 10% (wt/vol) inulin-type sugars derived from an extract of Jerusalem artichoke (Helianthus tuberosus) tubers, supplemented with small amounts of Tween 80, oleic acid, and corn steep liquor. Initial ethanol concentrations ranging from 0 to 80 g/liter in the liquid medium were used to study the inhibitory effect of ethanol on the following parameters: maximum specific growth rate (mu(max)), cell and ethanol yields, and sugar utilization. It was found that as the initial ethanol concentration increased from 0 to 80 g/liter, and maximum specific growth rate of K. marxianus cells decreased from 0.42 to 0.09 h, whereas the ethanol and cell yields and sugar utilization remained almost constant. A simple kinetic model was used to correlate the mu(max) results and the rates of cell and ethanol production, and the appropriate constants were evaluated.
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16346150
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10.1128/aem.44.6.1325-1329.1982
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P Bajpai; A Margaritis
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Applied and environmental microbiology
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1982
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2025-11-10T15:53:40.954600
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27857705
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pubmed_async
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literature
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Isolation, Identification and Characterization of Yeasts from Fermented Goat Milk of the Yaghnob Valley in Tajikistan.
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The geographically isolated region of the Yaghnob Valley, Tajikistan, has allowed its inhabitants to maintain a unique culture and lifestyle. Their fermented goat milk constitutes one of the staple foods for the Yaghnob population, and is produced by backslopping, i.e., using the previous fermentation batch to inoculate the new one. This study addresses the yeast composition of the fermented milk, assessing genotypic, and phenotypic properties. The 52 isolates included in this study revealed small species diversity, belonging to
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27857705
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10.19026/ajfst.7.1294
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Linnea A Qvirist; Carlotta De Filippo; Francesco Strati; Irene Stefanini; Maddalena Sordo; Thomas Andlid; Giovanna E Felis; Paola Mattarelli; Duccio Cavalieri
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Frontiers in microbiology
|
2016
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2025-11-10T15:53:40.955008
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27260810
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pubmed_async
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literature
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Oral yeast colonization in peritoneal dialysis and hemodialysis patients and renal transplant recipients.
|
We aimed to investigate the frequency of oral yeast colonization (OYC) and the risk factors for patients who received continuous ambulatory peritoneal dialysis (CAPD) or hemodialysis (HD) or were renal transplant recipients (RTRs). The patients admitted to the Nephrology Clinic at Ataturk University Medical School from January through April 2013 were included in the study. A questionnaire about risk factors was filled out, and swab cultures were taken from the tongue surface of each participant. OYC was detected in 32.1% of the RTRs, 40% of the HD patients, 20.9% of the CAPD patients, and 18% of the healthy control (HC) group. Of the 42 yeast strains isolated from the renal replacement therapy groups, 26 strains (61.9%) were Candida albicans, nine (21.4%) were Candida glabrata, two (4.7%) were Candida krusei, two (4.7%) were Candida kefyr, one (2.38%) was Candida parapsilosis, and two (4.7%) were Geotrichum candidum. Risk factors for OYC in the RTRs group included antibiotic use and the presence of dental prostheses; however, in patients with chronic renal failure undergoing CAPD, only the presence of dental prostheses was found to be a statistically significant risk factor. Although OYC was mostly detected in patients with chronic kidney disease (undergoing HD, a variety of isolated yeast strains in the RTRs was noted. The rates of OYC and isolated Candida species in CAPD were similar to those of the HC group.
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27260810
|
10.1016/j.cimid.2016.04.004
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Aynur Gulcan; Erim Gulcan; Mustafa Keles; Esin Aktas
|
Comparative immunology, microbiology and infectious diseases
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2016
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2025-11-10T15:53:40.955372
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29644852
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pubmed_async
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literature
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Improving the Organoleptic Properties of a Craft Mezcal Beverage by Increasing Fatty Acid Ethyl Ester Contents through ATF1 Expression in an Engineered Kluyveromyces marxianus UMPe-1 Yeast.
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Mezcal, a traditional beverage that originated in Mexico, is produced from species of the Agavaceae family. The esters associated with the yeasts utilized during fermentation are important for improving the organoleptic properties of the beverage. We improved the ester contents in a mezcal beverage by using the yeast Kluyveromyces marxianus, which was engineered with the ATF1 gene. ATF1 expression in the recombinant yeast significantly increased compared with that in the parental yeast, but its fermentative parameters were unchanged. Volatile-organic-compound-content analysis showed that esters had significantly increased in the mezcal produced with the engineered yeast. In a sensory-panel test, 48% of the panelists preferred the mezcal produced from the engineered yeast, 30% preferred the mezcal produced from the wild type, and 15 and 7% preferred the two mezcal types produced following the routine procedure. Correlation analysis showed that the fruitiness/sweetness description of the mezcal produced using the ATF1-engineered K. marxianus yeast correlated with the content of the esters, whose presence improved the organoleptic properties of the craft mezcal beverage.
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29644852
|
10.1021/acs.jafc.8b00730
|
Jesús Campos-García; Alejandra Vargas; Lorena Farías-Rosales; Ana L Miranda; Víctor Meza-Carmen; Alma L Díaz-Pérez
|
Journal of agricultural and food chemistry
|
2018
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2025-11-10T15:53:40.955771
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33167273
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pubmed_async
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literature
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An ion-pair free LC-MS/MS method for quantitative metabolite profiling of microbial bioproduction systems.
|
Data-driven engineering of microbes has been demonstrated for the sustainable production of high-performance chemicals. Metabolic profiling analysis is essential to increase the productivity of target compounds. However, improvement of comprehensive analysis methodologies is required for the high demands of metabolic engineering. Therefore, a liquid chromatography-tandem mass spectrometry (LC-MS/MS) based methodology was designed and applied to cover a wide target range with high precision. Ion-pair free separation of metabolites on a pentafluorophenyl propyl column enabled high-precision quantification of 113 metabolites. The method was further evaluated for high reproducibility and robustness. Target analytes consisted of primary metabolites and intermediate metabolites for microbial production of high-performance chemicals. 95 metabolites could be detected with high reproducibility of peak area (intraday data: CV<15%), and 53 metabolites could be sensitively determined within a wide dynamic linear range (3-4 orders of magnitude). The developed system was further applied to the metabolomic analysis of various prokaryotic and eukaryotic microorganisms. Differences due to culture media and metabolic phenotypes could be observed when comparing the metabolomes of conventional and non-conventional yeast. Furthermore, almost all Kluyveromyces marxianus metabolites could be detected with moderate reproducibility (CV<40%, among independent extractions), where 41 metabolites were detected with very high reproducibility (CV<15%). In addition, the accuracy was validated via a spike-and-recovery test,and 78 metabolites were detected with analyte recovery in the 80-120% range. Together these results establish ion-pair free metabolic profiling as a comprehensive and precise tool for data-driven bioengineering applications.
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33167273
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10.1016/j.talanta.2020.121625
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Musashi Takenaka; Takanobu Yoshida; Yoshimi Hori; Takahiro Bamba; Masao Mochizuki; Christopher J Vavricka; Takanari Hattori; Yoshihiro Hayakawa; Tomohisa Hasunuma; Akihiko Kondo
|
Talanta
|
2021
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2025-11-10T15:53:40.956126
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24462702
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pubmed_async
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literature
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Yeasts are essential for cocoa bean fermentation.
|
Cocoa beans (Theobroma cacao) are the major raw material for chocolate production and fermentation of the beans is essential for the development of chocolate flavor precursors. In this study, a novel approach was used to determine the role of yeasts in cocoa fermentation and their contribution to chocolate quality. Cocoa bean fermentations were conducted with the addition of 200ppm Natamycin to inhibit the growth of yeasts, and the resultant microbial ecology and metabolism, bean chemistry and chocolate quality were compared with those of normal (control) fermentations. The yeasts Hanseniaspora guilliermondii, Pichia kudriavzevii and Kluyveromyces marxianus, the lactic acid bacteria Lactobacillus plantarum and Lactobacillus fermentum and the acetic acid bacteria Acetobacter pasteurianus and Gluconobacter frateurii were the major species found in the control fermentation. In fermentations with the presence of Natamycin, the same bacterial species grew but yeast growth was inhibited. Physical and chemical analyses showed that beans fermented without yeasts had increased shell content, lower production of ethanol, higher alcohols and esters throughout fermentation and lesser presence of pyrazines in the roasted product. Quality tests revealed that beans fermented without yeasts were purplish-violet in color and not fully brown, and chocolate prepared from these beans tasted more acid and lacked characteristic chocolate flavor. Beans fermented with yeast growth were fully brown in color and gave chocolate with typical characters which were clearly preferred by sensory panels. Our findings demonstrate that yeast growth and activity were essential for cocoa bean fermentation and the development of chocolate characteristics.
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24462702
|
10.1016/j.ijfoodmicro.2013.12.014
|
Van Thi Thuy Ho; Jian Zhao; Graham Fleet
|
International journal of food microbiology
|
2014
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2025-11-10T15:53:40.956408
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39489214
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pubmed_async
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literature
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Engineering peroxisomal surface display for enhanced biosynthesis in the emerging yeast Kluyveromyces marxianus.
|
The non-conventional yeast Kluyveromyces marxianus is a promising microbial host for industrial biomanufacturing. With the recent development of Cas9-based genome editing systems and other novel synthetic biology tools for K. marxianus, engineering of this yeast has become far more accessible. Enzyme colocalization is a proven approach to increase pathway flux and the synthesis of non-native products. Here, we engineer K. marxianus to enable peroxisomal surface display, an enzyme colocalization technique for displaying enzymes on the peroxisome membrane via an anchoring motif from the peroxin Pex15. The native KmPex15 anchoring motif was identified and fused to GFP, resulting in successful localization to the surface of the peroxisomes. To demonstrate the advantages for pathway localization, the Pseudomonas savastanoi IaaM and IaaH enzymes were co-displayed on the peroxisome surface; this increased production of indole-3-acetic acid 7.9-fold via substrate channeling effects. We then redirected pathway flux by displaying the violacein pathway enzymes VioE and VioD from Chromobacterium violaceum, increasing selectivity of proviolacein to prodeoxyviolacein by 2.5-fold. Finally, we improved direct access to peroxisomal acetyl-CoA and increased titers of the polyketide triacetic acid lactone (TAL) by 2-fold through concurrent display of the proteins Cat2, Acc1, and the type III PKS 2-pyrone synthase from Gerbera hybrida relative to the same three enzymes diffusing in the cytosol. We further improved TAL production by up to 2.1-fold through engineering peroxisome morphology and lifespan. Our findings demonstrate that peroxisomal surface display is an efficient enzyme colocalization strategy in K. marxianus and applicable for improving production of a wide range of non-native products.
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39489214
|
10.1016/j.ymben.2024.10.014
|
Shane Bassett; Jonathan C Suganda; Nancy A Da Silva
|
Metabolic engineering
|
2024
|
2025-11-10T15:53:40.956785
|
|||||||
17420604
|
pubmed_async
|
literature
|
Effect of Kluyveromyces marxianus YIT 8292 crude cell wall fraction on serum lipids in normocholesterolemic and mildly hypercholesterolemic subjects.
|
The hypocholesterolemic effects of Kluyveromyces marxianus YIT 8292 crude cell wall (KM-CW) were examined. In pilot studies, KM-CW tablets were administered to mildly hypercholesterolemic subjects at doses of 8.0, 4.0, 2.0, or 1.0 g/d for 4 weeks. Total cholesterol (TC) and low-density lipoprotein cholesterol (LDL-C) decreased at doses above 2.0 and 4.0 g/d, respectively. Further, we examined the effect of intake of yogurt containing 3.0 or 4.0 g of KM-CW/d for 8 weeks in normal and hypercholesterolemic subjects in a double-blind placebo-controlled study. The intake of either of the KM-CW-containing yogurts was associated with significantly improved TC and LDL-C in hypercholesterolemic subjects, but had no effect on these levels in normal subjects. TC was significantly lower at week 8 in the hypercholesterolemic subjects who ingested yogurt containing 3.0 or 4.0 g of KM-CW than in those who consumed placebo yogurt. Intake of KM-CW might contribute to the prevention of hypercholesterolemia.
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17420604
|
10.1271/bbb.60539
|
Yasuto Yoshida; Eiichiro Naito; Kenji Ohishi; Takekazu Okumura; Masahiko Ito; Tadashi Sato; Haruji Sawada
|
Bioscience, biotechnology, and biochemistry
|
2007
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2025-11-10T15:53:40.957103
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25642435
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pubmed_async
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literature
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Dietary Yeasts Reduce Inflammation in Central Nerve System via Microflora.
|
The intestinal microflora affects the pathogenesis of several autoimmune diseases by influencing immune system function. Some bacteria, such as lactic acid bacteria, have been reported to have beneficial effects on immune function. However, little is known about the effects of yeasts. Here, we aimed to investigate the effects of various dietary yeasts contained in fermented foods on experimental autoimmune encephalomyelitis (EAE), an animal model of multiple sclerosis (MS), and to elucidate the mechanisms underlying these effects. The effects of eight yeasts selected from 18 types of yeasts contained in fermented foods were examined using an EAE model. Of these, Candida kefyr was investigated by analyzing the intestinal microflora and its effects on intestinal and systemic immune states. Administration of C. kefyr ameliorated the severity of EAE. Reduced numbers of Th17 cells, suppressed interleukin (IL)-6 production by intestinal explants, and increased Tregs and CD103-positive regulatory dendritic cells in mesenteric lymph nodes (MLNs) were observed. Analysis of 16s-rDNA from feces of C. kefyr-treated mice demonstrated increased Lactobacillales and decreased Bacteroides compared to control flora. Transfer of intestinal microbiota also resulted in decreased Bacteroides and ameliorated symptoms of EAE. Thus, oral administration of C. kefyr ameliorated EAE by altering the microflora, accompanied by increased Tregs and CD103-positive regulatory dendritic cells in MLNs and decreased Th17 cells in the intestinal lamina propria. Oral ingestion of C. kefyr may have beneficial effects on MS by modifying microflora. In addition, our findings also suggested the potential health benefits of dietary yeasts.
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25642435
|
10.1002/acn3.153
|
Kazushiro Takata; Takayuki Tomita; Tatsusada Okuno; Makoto Kinoshita; Toru Koda; Josephe A Honorat; Masaya Takei; Kouichiro Hagihara; Tomoyuki Sugimoto; Hideki Mochizuki; Saburo Sakoda; Yuji Nakatsuji
|
Annals of clinical and translational neurology
|
2015
|
2025-11-10T15:53:40.957541
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37081263
|
pubmed_async
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literature
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Identification and evaluation of thermotolerance of yeasts from milk in natura exposed to high temperature and slow and fast pasteurization.
|
Milk is considered one of the basic raw materials of animal origin; it must present hygienic quality and physical-chemical properties suitable for processing and human consumption. Thus, the ingestion of milk in natura when not properly treated can be characterized as an opportunistic route of transmission of possible microbial pathogens, which can offer risks to public health. The present study aimed the yeast identification, to analyze the thermo-resistance of yeasts isolated from fresh milk, and to trace the susceptibility profile of the isolates to antifungal agents. For this, 23 samples of fresh milk type B, collected by manual or mechanical milking, were stored in collective refrigeration tanks of farms located in the Metropolitan Region of Natal and nearby, State of Rio Grande do Norte (RN), Brazil. Twenty samples of fresh milk commercially traded in the city of Ceará-Mirim RN were also analyzed. The yeasts were quantified by count of colony-forming units (CFU). All isolated species were treated by slow pasteurization (62-64 °C for 30 min) and fast (72-75 °C for 20 s), as well as by boiling (100 °C). Fifty yeast strains were obtained, and the species were identified as Candida tropicalis (28%), Candida parapsilosis (14%), Candida albicans (12%), Candida glabrata (10%), Candida krusei (10%), Kluyveromyces marxianus (10%), Candida guilliermondii (8%), Candida rugosa (2%), Candida orthopsilosis (2%), Pichia manshurica (2%), and Kodamaea ohmeri (2%). Five isolates showed resistance to the antifungal agents tested. Among all the isolates submitted to heat treatment, 80% were resistant to fast pasteurization and 60% to boiling, but none of them resisted the slow pasteurization. The milk collected through mechanical milking and stored in collective cooling tanks, presented higher rates of yeast contamination, compared to milk samples collected by manual milking and kept under the same storage conditions.
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37081263
|
10.1016/j.ijfoodmicro.2019.04.006
|
Jéssica da Silva Campos; Antonio Carlos Vital Júnior; Douglas Boniek; Danielle Leticia da Silva; Ubiraci Gomes de Paula Lana; José Veríssimo Fernandes; Maria Aparecida de Resende Stoianoff; Vânia Sousa Andrade
|
Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology]
|
2023
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2025-11-10T15:53:40.957939
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40142402
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pubmed_async
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literature
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Alien Chromosome Serves as a Novel Platform for Multiple Gene Expression in
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40142402
|
10.1038/s42003-024-06487-w
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Yilin Lyu; Jungang Zhou; Yao Yu; Hong Lu
|
Microorganisms
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2025
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2025-11-10T15:53:40.958287
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9813735
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pubmed_async
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literature
|
Production of bioingredients from Kluyveromyces marxianus grown on whey: an alternative.
|
Whey waste is a major problem for the dairy industry. Finding alternative means to reduce its pollution potential and produce high value-added bioingredients has been attempted by many researchers. Kluyveromyces marxianus var. marxianus is a dairy yeast that produces beta-galactosidase, allowing for whey fermentation. Also, K. marxianus has been proposed as a source of: (1) oligonucleotides, used as flavor enhancers in food products; (2) oligosaccharides, used as prebiotics to stimulate the growth of Bifidobacterium sp. in the animal and human intestines; and (3) oligopeptides, immunostimulators added to dairy products that are released in the wort after whey protein proteolysis. Fed-batch fermentation can be used as an alternative process to avoid increases in lactose concentration and prevent the catabolite repression of the respiratory enzyme synthesis during aerobic fermentation, thus allowing for high biomass yields. The relevance of these factors on yeast fermentation of whey is summarized in this critical review.
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9813735
|
10.1080/10408699891274318
|
M A Belem; B H Lee
|
Critical reviews in food science and nutrition
|
1998
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2025-11-10T15:53:40.958518
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25910031
|
pubmed_async
|
literature
|
Phytase-producing capacity of yeasts isolated from traditional African fermented food products and PHYPk gene expression of Pichia kudriavzevii strains.
|
Phytate is known as a strong chelate of minerals causing their reduced uptake by the human intestine. Ninety-three yeast isolates from traditional African fermented food products, belonging to nine species (Pichia kudriavzevii, Saccharomyces cerevisiae, Clavispora lusitaniae, Kluyveromyces marxianus, Millerozyma farinosa, Candida glabrata, Wickerhamomyces anomalus, Hanseniaspora guilliermondii and Debaryomyces nepalensis) were screened for phytase production on solid and liquid media. 95% were able to grow in the presence of phytate as sole phosphate source, P. kudriavzevii being the best growing species. A phytase coding gene of P. kudriavzevii (PHYPk) was identified and its expression was studied during growth by RT-qPCR. The expression level of PHYPk was significantly higher in phytate-medium, compared to phosphate-medium. In phytate-medium expression was seen in the lag phase. Significant differences in gene expression were detected among the strains as well as between the media. A correlation was found between the PHYPk expression and phytase extracellular activity.
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25910031
|
10.1016/j.ijfoodmicro.2015.04.011
|
Anna Greppi; Łukasz Krych; Antonella Costantini; Kalliopi Rantsiou; D Joseph Hounhouigan; Nils Arneborg; Luca Cocolin; Lene Jespersen
|
International journal of food microbiology
|
2015
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2025-11-10T15:53:40.958846
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27260286
|
pubmed_async
|
literature
|
Kluyveromyces marxianus as a host for heterologous protein synthesis.
|
The preferentially respiring and thermotolerant yeast Kluyveromyces marxianus is an emerging host for heterologous protein synthesis, surpassing the traditional preferentially fermenting yeast Saccharomyces cerevisiae in some important aspects: K . marxianus can grow at temperatures 10 °C higher than S. cerevisiae, which may result in decreased costs for cooling bioreactors and reduced contamination risk; has ability to metabolize a wider variety of sugars, such as lactose and xylose; is the fastest growing eukaryote described so far; and does not require special cultivation techniques (such as fed-batch) to avoid fermentative metabolism. All these advantages exist together with a high secretory capacity, performance of eukaryotic post-translational modifications, and with a generally regarded as safe (GRAS) status. In the last years, replication origins from several Kluyveromyces spp. have been used for the construction of episomal vectors, and also integrative strategies have been developed based on the tendency for non-homologous recombination displayed by K. marxianus. The recessive URA3 auxotrophic marker and the dominant Kan(R) are mostly used for selection of transformed cells, but other markers have been made available. Homologous and heterologous promoters and secretion signals have been characterized, with the K. marxianus INU1 expression and secretion system being of remarkable functionality. The efficient synthesis of roughly 50 heterologous proteins has been demonstrated, including one thermophilic enzyme. In this mini-review, we summarize the physiological characteristics of K. marxianus relevant for its use in the efficient synthesis of heterologous proteins, the efforts performed hitherto in the development of a molecular toolbox for this purpose, and some successful examples.
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27260286
|
10.1007/s00253-016-7645-y
|
Andreas K Gombert; José Valdo Madeira; María-Esperanza Cerdán; María-Isabel González-Siso
|
Applied microbiology and biotechnology
|
2016
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2025-11-10T15:53:40.959142
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|||||||
33813131
|
pubmed_async
|
literature
|
Use of non-Saccharomyces yeasts in cider fermentation: Importance of the nutrients addition to obtain an efficient fermentation.
|
The isolation of autochthonous yeast species presents a good strategy to select new microorganisms for developing an adequate inoculum to carry out fermentations and generate representative products of the cider production zone. However, non-Saccharomyces yeasts have been considered to have low capacity to carry out a complete fermentation as Saccharomyces cerevisiae. In this work, five autochthonous yeasts from a cider fermentation process were isolated and identified as Saccharomyces cerevisiae, Kluyveromyces marxianus, Pichia membranaefaciens, P. kluyveri and Zygosaccharomyces rouxii. A series of fermentations were developed at laboratory level, using each species individually and it was observed that only S. cerevisiae was able to finish the process. K. marxianus consumed less than 50% of the sugars; P. kluyveri and Z. rouxii consumed less than 70% and P. membranaefaciens consumed more than 90% but the yield (ethanol produced for sugar consumed (Y
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33813131
|
10.1016/j.ijfoodmicro.2021.109169
|
Anne Gschaedler; Laura E Iñiguez-Muñoz; Nilda Y Flores-Flores; Manuel Kirchmayr; Melchor Arellano-Plaza
|
International journal of food microbiology
|
2021
|
2025-11-10T15:53:40.959422
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16995526
|
pubmed_async
|
literature
|
A reverse transcriptase PCR technique for the detection and viability assessment of Kluyveromyces marxianus in yoghurt.
|
A fast and sensitive reverse transcriptase PCR (RT-PCR) method was developed for the detection of viable Kluyveromyces marxianus in yoghurt. Yeast-specific primers were used with the RT-PCR to evaluate the suitability of 18S rRNA as a target for the detection of viable yeasts in pure culture and yoghurt. The RT-PCR assay was able to detect down to 10(2) CFU ml(-1) in yoghurt samples contaminated with viable yeast cells. Application of the RT-PCR method to commercial yoghurt samples demonstrated the utility of this technique for detection of low concentrations of viable yeast cells in naturally contaminated dairy products. The 18S rRNA molecule is an appropriate target for cell viability assessment because of its limited persistence after cell death and the resultant high level of sensitivity of the assay.
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16995526
|
10.4315/0362-028x-69.9.2210
|
María Belén Mayoral; Rosario Martin; Pablo E Hernández; Isabel González; Teresa García
|
Journal of food protection
|
2006
|
2025-11-10T15:53:40.959731
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33661900
|
pubmed_async
|
literature
|
Construction of engineered RuBisCO Kluyveromyces marxianus for a dual microbial bioethanol production system.
|
Ribulose-1,5-bisphosphate carboxylase/oxygenase (RubisCO) genes play important roles in CO2 fixation and redox balancing in photosynthetic bacteria. In the present study, the kefir yeast Kluyveromyces marxianus 4G5 was used as host for the transformation of form I and form II RubisCO genes derived from the nonsulfur purple bacterium Rhodopseudomonas palustris using the Promoter-based Gene Assembly and Simultaneous Overexpression (PGASO) method. Hungateiclostridium thermocellum ATCC 27405, a well-known bacterium for its efficient solubilization of recalcitrant lignocellulosic biomass, was used to degrade Napier grass and rice straw to generate soluble fermentable sugars. The resultant Napier grass and rice straw broths were used as growth media for the engineered K. marxianus. In the dual microbial system, H. thermocellum degraded the biomass feedstock to produce both C5 and C6 sugars. As the bacterium only used hexose sugars, the remaining pentose sugars could be metabolized by K. marxianus to produce ethanol. The transformant RubisCO K. marxianus strains grew well in hydrolyzed Napier grass and rice straw broths and produced bioethanol more efficiently than the wild type. Therefore, these engineered K. marxianus strains could be used with H. thermocellum in a bacterium-yeast coculture system for ethanol production directly from biomass feedstocks.
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33661900
|
10.1186/s12866-014-0215-5
|
Dung Minh Ha-Tran; Rou-Yin Lai; Trinh Thi My Nguyen; Eugene Huang; Shou-Chen Lo; Chieh-Chen Huang
|
PloS one
|
2021
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2025-11-10T15:53:40.960065
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|||||||
39464777
|
pubmed_async
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literature
|
Occurrence of pathogenic yeast
|
Yeasts are widely known for their application in food production, but also because of their clinical significance. As human pathogens, several species of yeasts, mainly of the genus
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39464777
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10.1093/ofid/ofac559
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Zilpa Adriana Sánchez Quitian; Guisell Mariana Pérez Rozo; Carolina Firacative
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F1000Research
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2024
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2025-11-10T15:53:40.960584
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35283845
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pubmed_async
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literature
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Survivability of
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35283845
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10.1007/s12602-021-09872-7
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Hye-Young Youn; Dong-Hyeon Kim; Hyeon-Jin Kim; Dongryeoul Bae; Kwang-Young Song; Hyunsook Kim; Kun-Ho Seo
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Frontiers in microbiology
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2022
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2025-11-10T15:53:40.960944
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27164865
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pubmed_async
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literature
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Enhanced exo-inulinase activity and stability by fusion of an inulin-binding module.
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In this study, an inulin-binding module from Bacillus macerans was successfully fused to an exo-inulinase from Kluyveromyces marxianus, creating a hybrid functional enzyme. The recombinant exo-inulinase (rINU), the hybrid enzyme (rINUIBM), and the recombinant inulin-binding module (rIBM) were, respectively, heterologously expressed and biochemically characterized. It was found that both the inulinase activity and the catalytic efficiency (k cat/K m(app)) of the rINUIBM were considerably higher than those of rINU. Though the rINU and the rINUIBM shared the same optimum pH of 4.5, the optimum temperature of the rINUIBM (60 °C) was 5 °C higher than that of the rINU. Notably, the fused IBM significantly enhanced both the pH stability and the thermostability of the rINUIBM, suggesting that the rINUIBM obtained would have more extensive potential applications. Furthermore, the fusion of the IBM could substantially improve the inulin-binding capability of the rINUIBM, which was consistent with the determination of the K m(app). This meant that the fused IBM could play a critical role in the recognition of polysaccharides and enhanced the hydrolase activity of the associated inulinase by increasing enzyme-substrate proximity. Besides, the extra supplement of the independent non-catalytic rIBM could also improve the inulinase activity of the rINU. However, this improvement was much better in case of the fusion. Consequently, the IBM could be designated as a multifunctional domain that was responsible for the activity enhancement, the stabilization, and the substrate binding of the rINUIBM. All these features obtained in this study make the rINUIBM become an attractive candidate for an efficient inulin hydrolysis.
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27164865
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10.1007/s00253-016-7587-4
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Shun-Hua Zhou; Yuan Liu; Yu-Juan Zhao; Zhe Chi; Zhen-Ming Chi; Guang-Lei Liu
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Applied microbiology and biotechnology
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2016
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2025-11-10T15:53:40.961227
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19939673
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pubmed_async
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literature
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Ethanol production through simultaneous saccharification and fermentation of switchgrass using Saccharomyces cerevisiae D(5)A and thermotolerant Kluyveromyces marxianus IMB strains.
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Hydrothermolysis pretreated switchgrass at 200 degrees C for 10min was used in a simultaneous saccharification and fermentation (SSF) process using five thermotolerant yeast strains Kluyveromyces marxianus IMB 1, IMB 2, IMB 3, IMB 4, and IMB 5 at 45 degrees C and Saccharomyces cerevisiae D(5)A at 37 degrees C. SSF was carried out for 7d using 5, 10, and 15FPU/g glucan to determine the effect of decreasing cellulase loading on ethanol yield. The effect of initial pH on SSF by S. cerevisiae D(5)A was also investigated. Fermentation by K. marxianus IMB 1, IMB 2, IMB 4, and IMB 5 ceased by 72 h and fermentation by K. marxianus IMB 3 ceased by 96 h, while fermentation S. cerevisiae D(5)A continued for 7d. At 96 and 120 h, IMB 3 and S. cerevisiae D(5)A had similar ethanol yields while the other K. marxianus strains were lower at a 95% confidence level. Final ethanol yields for IMB 3, IMB 1, IMB 5 strains were similar to one another, however, ethanol yield for S. cerevisiae D(5)A (92% maximum theoretical) was greater than all of the IMB strains except IMB 3 at a 95% confidence level. Reducing enzyme loading reduced ethanol yields for both K. marxianus IMB 3 and S. cerevisiae D(5)A. Reducing buffer pH from 5.5 to 4.8 reduced ethanol yields for S. cerevisiae D(5)A. This study shows that K. marxianus IMB 3 has potential for commercial use for ethanol production from cellulose in SSF processes with further improvement of its thermotolerance.
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19939673
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10.1016/j.biortech.2009.11.001
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Brian A Faga; Mark R Wilkins; Ibrahim M Banat
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Bioresource technology
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2010
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2025-11-10T15:53:40.961507
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35218085
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pubmed_async
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literature
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A novel transcriptional activation mechanism of inulinase gene in Kluyveromyces marxianus involving a glycolysis regulator KmGcr1p with unique and functional Q-rich repeats.
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Kluyveromyces marxianus is the most suitable fungus for inulinase industrial production. However, the underlying transcriptional activation mechanism of the inulinase gene (INU1) is hitherto unclear. Here, we undertook genetic and biochemical analyses to elucidate that a glycolysis regulator KmGcr1p with unique Q-rich repeats is the key transcriptional activator of INU1. We determined that INU1 and glycolytic genes share similar transcriptional activation patterns and that inulinase activity is induced by fermentable carbon sources including the hydrolysis products of inulin (fructose and glucose), which suggests a novel model of product feedback activation. Furthermore, all four CT-boxes in the INU1 promoter are important for KmGcr1p DNA-binding in vitro, but the most downstream CT-box 1 primarily confers upstream activating sequence activity in vivo. More intriguingly, the use of artificial and natural GCR1 mutants suggests that the Q-rich repeats act as a functional module to maintain KmGcr1p transcriptional activity by contributing to its solubility and DNA-binding affinity. Altogether, this study uncovers a novel transcriptional activation mechanism for the inulinase gene, that is different from the previous understanding for filamentous fungi, but might have universal significance among inulinase-producing yeasts, thereby leading to a better understanding of the regulation mechanism of yeast inulinase genes.
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35218085
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10.1111/mmi.14889
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Fengyi Li; Mengqi Wang; Zhe Chi; Zhaoxuan Zhang; Xiaoxiang Wang; Mengdan Xing; Zhenming Chi; Guanglei Liu
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Molecular microbiology
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2022
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2025-11-10T15:53:40.961823
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27130462
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pubmed_async
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literature
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Growth, ethanol production, and inulinase activity on various inulin substrates by mutant Kluyveromyces marxianus strains NRRL Y-50798 and NRRL Y-50799.
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Economically important plants contain large amounts of inulin. Disposal of waste resulting from their processing presents environmental issues. Finding microorganisms capable of converting inulin waste to biofuel and valuable co-products at the processing site would have significant economic and environmental impact. We evaluated the ability of two mutant strains of Kluyveromyces marxianus (Km7 and Km8) to utilize inulin for ethanol production. In glucose medium, both strains consumed all glucose and produced 0.40 g ethanol/g glucose at 24 h. In inulin medium, Km7 exhibited maximum colony forming units (CFU)/mL and produced 0.35 g ethanol/g inulin at 24 h, while Km8 showed maximum CFU/mL and produced 0.02 g ethanol/g inulin at 96 h. At 24 h in inulin + glucose medium, Km7 produced 0.40 g ethanol/g (inulin + glucose) and Km8 produced 0.20 g ethanol/g (inulin + glucose) with maximum CFU/mL for Km8 at 72 h, 40 % of that for Km7 at 36 h. Extracellular inulinase activity at 6 h for both Km7 and Km8 was 3.7 International Units (IU)/mL.
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27130462
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10.1111/j.1365-2672.2008.03903.x
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Luz Ángela Galindo-Leva; Stephen R Hughes; Juan Carlos López-Núñez; Joshua M Jarodsky; Adam Erickson; Mitchell R Lindquist; Elby J Cox; Kenneth M Bischoff; Eric C Hoecker; Siqing Liu; Nasib Qureshi; Marjorie A Jones
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Journal of industrial microbiology & biotechnology
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2016
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2025-11-10T15:53:40.962173
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30061929
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pubmed_async
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literature
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Engineering TATA-binding protein Spt15 to improve ethanol tolerance and production in
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Low ethanol tolerance of A random mutagenesis library of This work demonstrates that ethanol tolerance of
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30061929
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10.1093/nar/gkw937
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Pengsong Li; Xiaofen Fu; Shizhong Li; Lei Zhang
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Biotechnology for biofuels
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2018
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2025-11-10T15:53:40.962526
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20820924
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pubmed_async
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literature
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Sequence analysis and structural characterization of a glyceraldehyde-3-phosphate dehydrogenase gene from the phytopathogenic fungus Eremothecium ashbyi.
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Eremothecium ashbyi is a phytopathogenic fungus infesting cotton, soybeans and several other plants. This highly flavinogenic fungus has been phylogenetically characterized, but the genetic aspects of its central metabolic and riboflavin biosynthetic pathways are unknown. An ORF of 996 bp was obtained from E. ashbyi by using degenerate primers for glyceraldehyde-3-phosphate dehydrogenase (GPD) through reverse transcriptase polymerase chain reaction (RT-PCR) and 5'-3' rapid amplification of cDNA ends (RACE-PCR). This nucleotide sequence had a high similarity of 88% with GPD sequence of Ashbya gossypii. The putative GPD peptide of 331-aa had a high similarity of 85% with the GPD sequence from other ascomycetes. The ORF had an unusually strong codon bias with 5 amino acids showing strict preference of a single codon. The theoretical molecular weight for the putative peptide was 35.58 kDa with an estimated pI of 5.7. A neighbor-joining tree showed that the putative peptide from E. ashbyi displayed the highest similarity to GPD of A. gossypii. The gene sequence is available at the GenBank, accession number EU717696. Homology modeling done with Kluyveromyces marxianus GPD (PDB: 2I5P) as template indicated high structural similarity.
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20820924
|
10.1007/s11046-010-9357-7
|
Sudeshna Sengupta; T S Chandra
|
Mycopathologia
|
2011
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2025-11-10T15:53:40.962859
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32238769
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pubmed_async
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literature
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Comparison of Ethanol Yield Coefficients Using
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The red seaweed
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32238769
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10.1007/s10499-017-0120-7
|
Yurim Park; In Yung Sunwoo; Jiwon Yang; Gwi-Teak Jeong; Sung-Koo Kim
|
Journal of microbiology and biotechnology
|
2020
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2025-11-10T15:53:40.963181
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14529965
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pubmed_async
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literature
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Acquisition of flocculation phenotype by Kluyveromyces marxianus when overexpressing GAP1 gene encoding an isoform of glyceraldehyde-3-phosphate dehydrogenase.
|
The use of flocculating yeast strains has been considered as a convenient approach to obtain high cell densities in bioreactors with increasing productivity in continuous operations. In Kluyveromyces marxianus ATTC 10022, the GAP1 gene encodes an isoform of glyceraldehyde-3-phosphate dehydrogenase-p37-that is accumulated in the cell wall and is involved in flocculation. To test the use of p37 as a tool for engineering Kluyveromyces cells to display a flocculation phenotype, K. marxianus CCT 3172 was transformed with an expression vector containing GAP1. This vector is based on the pY37 previously described, harbouring a S11 Kluyveromyces origin of replication, and the expression of GAP1 is under the control of GAL1. Kluyveromyces cells overexpressing GAP1 acquired a flocculent phenotype together with the accumulation of p37 in the cell wall. The results support the use of GAP1 gene as a molecular tool for inducing flocculation.
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14529965
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10.1016/s0167-7012(03)00189-1
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Catarina Almeida; Odília Queirós; Alan Wheals; José Teixeira; Pedro Moradas-Ferreira
|
Journal of microbiological methods
|
2003
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2025-11-10T15:53:40.963449
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33716613
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pubmed_async
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literature
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A new approach for balancing the microbial synthesis of ethyl acetate and other volatile metabolites during aerobic bioreactor cultivations.
|
Ethyl acetate is an organic solvent with many industrial applications, currently produced by energy-intensive chemical processes based on fossil carbon resources. Ethyl acetate can be synthesized from renewable sugars by yeasts like
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33716613
|
10.1002/elsc.202000047
|
Christian Löser; Christian Kupsch; Thomas Walther; Andreas Hoffmann
|
Engineering in life sciences
|
2021
|
2025-11-10T15:53:40.963782
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23587421
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pubmed_async
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literature
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Thermal adaptability of Kluyveromyces marxianus in recombinant protein production.
|
Kluyveromyces marxianus combines the ease of genetic manipulation and fermentation with the ability to efficiently secrete high molecular weight proteins, performing eukaryotic post-translational modifications. It is able to grow efficiently in a wide range of temperatures. The secretion performances were analyzed in the host K. marxianus L3 in the range between 5°C and 40°C by means of 3 different reporter proteins, since temperature appears a key parameter for production and secretion of recombinant proteins. The recombinant strains were able to grow up to 40°C and, along the tested temperature interval (5-40°C), the specific growth rates (μ) were generally lower as compared to those of the untransformed strain. Biomass yields were slightly affected by temperature, with the highest values reached at 15°C and 30°C. The secretion of the endogenous β-fructofuranosidase, used as an internal control, was efficient in the range of the tested temperature, as evaluated by assaying the enzyme activity in the culture supernatants. The endogenous β-fructofuranosidase production was temperature dependent, with the highest yield at 30°C. The heterologous proteins HSA, GAA and Sod1p were all successfully produced and secreted between 5°C and 40°C, albeit each one presented a different optimal production temperature (15, 40, 5-30°C for HSA, GAA and Sod1p, respectively). K. marxianus L3 has been identified as a promising and flexible cell factory. In a sole host, the optimization of growth temperatures for the efficient secretion of each individual protein can be carried out over a wide range of temperatures.
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23587421
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10.1073/pnas.68.5.1024
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Stefano Raimondi; Elena Zanni; Alberto Amaretti; Claudio Palleschi; Daniela Uccelletti; Maddalena Rossi
|
Microbial cell factories
|
2013
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2025-11-10T15:53:40.964106
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33570102
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pubmed_async
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literature
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Unexpected Diversity of Yeast Species in Esophageal Mycosis of Waterfowls.
|
This study was performed to evaluate the diversity and prevalence of yeasts associated with esophageal mycosis in domestic ducks and geese. Fungi were isolated from esophageal lesions of dead animals sent for microbiologic laboratory diagnosis. Species identification using a culture-dependent method was carried out by sequencing of the internal transcribed spacer (ITS)1-5.8S rRNA-ITS2 region. The most frequently isolated yeast was Candida albicans (43.1%) followed by Saccharomyces cerevisiae (17.6%), Candida kefyr (11.7%), Kazachstania bovina (11.7%), Candida lambica (3.9%), and single isolates (1.9%) representing Candida inconspicua, Candida rugosa, Candida pelliculosa, Candida krusei, Magnusiomyces capitatus, and Trichosporon asahii. Our results indicate that a number of potentially pathogenic yeast species can be isolated from esophageal mycosis of waterfowls, but additional studies are needed to make conclusions regarding their possible etiologic role in disease.
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33570102
|
10.1637/aviandiseases-D20-00053
|
Marianna Domán; László Makrai; Krisztina Bali; György Lengyel; Tibor Laukó; Krisztián Bányai
|
Avian diseases
|
2020
|
2025-11-10T15:53:40.964397
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2321931
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pubmed_async
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literature
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Modes of lactose uptake in the yeast species Kluyveromyces marxianus.
|
Twelve lactose-assimilating strains of the yeast species Kluyveromyces marxianus and its varieties marxianus, lactis and bulgaricus were studied with respect to transport mechanisms for lactose, glucose and galactose, fermentation of these sugars and the occurrence of extracellular lactose hydrolysis. The strains fell into three groups. Group I (two strains): Fermentation of lactose, glucose and galactose, extracellular lactose hydrolysis, apparent facilitated diffusion of glucose and galactose; Group II (two strains): Lactose not fermented, glucose and galactose fermented and transported by an apparent proton symport, extracellular hydrolysis of lactose present (one strain) or questionable; Group III (eight strains): Lactose, glucose and galactose fermented, lactose transported by an apparent proton symport mechanism, extracellular hydrolysis of lactose and transport modes for glucose and galactose variable.
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2321931
|
10.1007/BF00403158
|
M Carvalho-Silva; I Spencer-Martins
|
Antonie van Leeuwenhoek
|
1990
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2025-11-10T15:53:40.964657
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8064542
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pubmed_async
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literature
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Rapid presumptive identification of medically relevant yeasts to the species level by polymerase chain reaction and restriction enzyme analysis.
|
A method for the rapid presumptive differentiation of a panel of 12 clinically relevant yeasts to the species level was developed on the basis of evaluation by the polymerase chain reaction (PCR) of the gene coding for the small ribosomal subunit 18S-rRNA. The method involved restriction enzyme analysis of PCR products obtained with primers common to all fungi. Using six restriction enzymes, AluI, BanI, BbsI, DraII, Eco147I and NheI, characteristic PCR-restriction enzyme patterns were obtained for Candida albicans, Candida tropicalis, Candida krusei, Candida kefyr, Candida lusitaniae, Candida guilliermondii, Candida glabrata and Saccharomyces cerevisiae, as well as for the pairs Candida parapsilosis/Candida viswanathii and Trichosporon beigelii/Cryptococcus neoformans. The procedure does not involve hybridization steps or the use of radioactivity and can be completed within one working day.
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8064542
|
10.1080/02681219480000161
|
M Maiwald; R Kappe; H G Sonntag
|
Journal of medical and veterinary mycology : bi-monthly publication of the International Society for Human and Animal Mycology
|
1994
|
2025-11-10T15:53:40.964908
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21067917
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pubmed_async
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literature
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Purification and substrate specificities of a fructanase from Kluyveromyces marxianus isolated from the fermentation process of Mezcal.
|
A fructanase, produced by a Kluyveromyces marxianus strain isolated during the fermentation step of the elaboration process of "Mezcal de Guerrero" was purified and biochemically characterized. The active protein was a glycosylated dimer with a molecular weight of approximately 250 kDa. The specific enzymatic activity of the protein was determined for different substrates: sucrose, inulin, Agave tequilana fructan, levan and Actilight® and compared with the activity of Fructozyme®. The hydrolysis profile of the different substrates analyzed by HPAEC-PAD showed that the enzyme has different affinities over the substrates tested with a sucrose/inulin enzymatic activity ratio (S/I) of 125. For the hydrolysis of Agave tequilana fructans, the enzyme also showed a higher enzymatic activity and specificity than Fructozyme®, which is important for its potential application in the tequila industry.
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21067917
|
10.1016/j.biortech.2010.10.071
|
Javier Arrizon; Sandrine Morel; Anne Gschaedler; Pierre Monsan
|
Bioresource technology
|
2011
|
2025-11-10T15:53:40.965158
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34864183
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pubmed_async
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literature
|
Consolidated bioprocessing of lactose into lactic acid and ethanol using non-engineered cell factories.
|
The aim of this study is the consolidated bioprocessing of lactose into lactic acid and ethanol using non-engineered Cell Factories (CFs). Therefore, two different types of composite biocatalysts (CF1-CF2) based on Saccharomyces cerevisiae with immobilized microorganism or enzyme on starch gel (SG) were prepared for 5% w/v lactose fermentation. In CF1, S. cerevisiae was covered with SG containing Lactobacillus casei, Lactobacillus bulgaricus, Kluyveromyces marxianus CF1a-c. S. cerevisiae/SG-β-galactosidase (CF1d) was also used for simultaneous saccharification and fermentation (SSF) of lactose. In CF2, S. cerevisiae immobilized on tubular cellulose (TC) was covered with SG containing the aforementioned microorganisms (CF2a-c). The wet CF1d resulted in 96% of the theoretical ethanol yield while the wet CF1b and freeze-dried CF2b resulted in 89% of the theoretical lactic acid yield. The repeated batches using the CF2a-c exhibited better results than using CF1a-c. Subsequently, the freeze-dried CF2 as preservative and more manageable were verified for future exploitation of whey.
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34864183
|
10.1016/j.biortech.2021.126464
|
Vassilios Panagopoulos; Konstantina Boura; Agapi Dima; Ioannis K Karabagias; Loulouda Bosnea; Poonam S Nigam; Maria Kanellaki; Athanasios A Koutinas
|
Bioresource technology
|
2022
|
2025-11-10T15:53:40.965442
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34125995
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pubmed_async
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literature
|
Fluorescence-based screening for engineered aldo-keto reductase KmAKR with improved catalytic performance and extended substrate scope.
|
Aldo-keto reductases-catalyzed transformations of ketones to chiral alcohols have become an established biocatalytic process step in the pharmaceutical industry. Previously, we have discovered an aldo-keto reductase (AKR) from Kluyveromyces marxianus that is active to the aliphatic tert-butyl 6-substituted (5R/S)-hydroxy-3-oxohexanoates, but it is inactive to aromatic ketones. In order to acquire an excellent KmAKRmutant for ensuring the simultaneous improvement of activity-thermostability toward tert-butyl 6-cyano-(5R)-hydroxy-3-oxohexanoate ((5R)-1) and broadening the universal application prospects toward more substrates covering both aliphatic and aromatic ketones, a fluorescence-based high-throughput (HT) screening technique was established. The directed evolution of KmAKR variant M5 (KmAKR-W297H/Y296W/K29H/Y28A/T63M) produced the "best" variant M5-Q213A/T23V. It exhibited enhanced activity-thermostability toward (5R)-1, improved activity toward all 18 test substrates and strict R-stereoselectivity toward 10 substrates in comparison to M5. The enhancement of enzymatic activity and the extension of substrate scope covering aromatic ketones are proposed to be largely attributed to pushing the binding pocket of M5-Q213A/T23V to the enzyme surface, decreasing the steric hindrance at the entrance and enhancing the flexibility of loops surrounding the active center. In addition, combined with 0.94 g dry cell weight (DCW) L A fluorescence-based HT screening system was developed to tailor KmAKR's activity, thermostability and substrate scope. The "best" variant M5-Q213A/T23V holds great potential application for the synthesis of aliphatic/aromatic R-configuration alcohols.
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34125995
|
10.1002/biot.202100130
|
Shuai Qiu; Shen-Yuan Xu; Shu-Fang Li; Kang-Ming Meng; Feng Cheng; Ya-Jun Wang; Yu-Guo Zheng
|
Biotechnology journal
|
2021
|
2025-11-10T15:53:40.965755
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28542187
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pubmed_async
|
literature
|
Elucidation of new condition-dependent roles for fructose-1,6-bisphosphatase linked to cofactor balances.
|
The cofactor balances in metabolism is of paramount importance in the design of a metabolic engineering strategy and understanding the regulation of metabolism in general. ATP, NAD+ and NADP+ balances are central players linking the various fluxes in central metabolism as well as biomass formation. NADP+ is especially important in the metabolic engineering of yeasts for xylose fermentation, since NADPH is required by most yeasts in the initial step of xylose utilisation, including the fast-growing Kluyveromyces marxianus. In this simulation study of yeast metabolism, the complex interplay between these cofactors was investigated; in particular, how they may affect the possible roles of fructose-1,6-bisphosphatase, the pentose phosphate pathway, glycerol production and the pyruvate dehydrogenase bypass. Using flux balance analysis, it was found that the potential role of fructose-1,6-bisphosphatase was highly dependent on the cofactor specificity of the oxidative pentose phosphate pathway and on the carbon source. Additionally, the excessive production of ATP under certain conditions might be involved in some of the phenomena observed, which may have been overlooked to date. Based on these findings, a strategy is proposed for the metabolic engineering of a future xylose-fermenting yeast for biofuel production.
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28542187
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10.1016/j.bbrc.2010.03.133
|
Du Toit W P Schabort; Stephanus G Kilian; James C du Preez
|
PloS one
|
2017
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2025-11-10T15:53:40.966099
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16397768
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pubmed_async
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literature
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An aqueous-organic two-phase bioprocess for efficient production of the natural aroma chemicals 2-phenylethanol and 2-phenylethylacetate with yeast.
|
The natural aroma chemicals 2-phenylethanol (2-PE) and 2-phenylethylacetate (2-PEAc) are of high industrial relevance and can be produced from L-phenylalanine in a yeast-based process with growth-associated product formation. Due to product inhibition, in situ product removal is mandatory to obtain economically interesting concentrations. A fed-batch approach using polypropylene glycol 1200 as in situ extractant and the precursor in a saturated concentration led to the highest 2-PE productivity reported for a bioprocess so far. With Kluyveromyces marxianus CBS 600, 26.5 g/l 2-PE and 6.1 g/l 2-PEAc in the organic phase were obtained, corresponding to space-time yields of 0.33 and 0.08 g/l h, respectively.
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16397768
|
10.1007/s00253-005-0281-6
|
M M W Etschmann; J Schrader
|
Applied microbiology and biotechnology
|
2006
|
2025-11-10T15:53:40.966363
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25614449
|
pubmed_async
|
literature
|
Bioflavour production from tomato and pepper pomaces by Kluyveromyces marxianus and Debaryomyces hansenii.
|
Bioflavours are called natural flavour and/or fragrance compounds which are produced using metabolic pathway of the microorganism and/or plant cells or their enzyme systems with bioengineering approaches. The aim of this study was to investigate bioflavour production from tomato and red pepper pomaces by Kluyveromyces marxianus and Debaryomyces hansenii. Obtained specific growth rates of K. marxianus and D. hansenii in tomato pomace were 0.081/h and 0.177/h, respectively. The bioflavour profile differed between the yeasts. Both yeasts can produce esters and alcohols such as phenyl ethyl alcohol, isoamyl alcohol, isoamyl acetate, phenyl ethyl acetate and isovaleric acid. "Tarhana" and "rose" were descriptive flavour terms for tomato and pepper pomaces fermented by K. marxianus, respectively. Tomato pomace fermented by D. hansenii had the most intense "green bean" flavour while "fermented vegetable" and "storage/yeast" were defined as characteristic flavour terms for pepper pomaces fermented by D. hansenii.
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25614449
|
10.1007/s00449-015-1356-0
|
Onur Güneşer; Aslı Demirkol; Yonca Karagül Yüceer; Sine Özmen Toğay; Müge İşleten Hoşoğlu; Murat Elibol
|
Bioprocess and biosystems engineering
|
2015
|
2025-11-10T15:53:40.966630
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31172988
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pubmed_async
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literature
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Evaluation of Kluyveromyces marxianus endo-polygalacturonase activity through ATR-FTIR.
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The endo-polygalacturonase enzyme (endoPG: EC 3.2.1.15) plays an important role in the fruit juice and wine industries, so the development of new tools for the quantitative and qualitative analysis of its enzymatic action is necessary. In this work, we report the development of a simple, fast and practical method that did not use any chemical reagent to identify and evaluate the action of the endoPG enzyme, produced by the yeast Kluyveromyces marxianus CCT3172, using attenuated total reflection Fourier-transform infrared (ATR-FTIR) spectroscopy combined with principal component analysis-linear discriminant analysis (PCA-LDA). This method evaluated the action of the endoPG enzyme on the polygalacturonic acid (PGA) substrate at 5 different times (0, 10, 15, 20 and 30 minutes), and at each time interval the samples were analyzed by ATR-FTIR. It was demonstrated that there was clear segregation between the samples that were and that were not subjected to the action of the endoPG enzyme, and it was also possible to distinguish the samples that were subjected to different incubation times with the enzyme. Through PCA-LDA it was possible to obtain wavelengths that are biomarkers for this enzymatic reaction and the observed changes as a function of hydrolysis duration were found to be in agreement with the breakdown of the glycosidic chain (1011 cm
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31172988
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10.1039/c9an00265k
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Felipe Raposo Passos Mansoldo; Athayde Neves Junior; Veronica da Silva Cardoso; Maria do Socorro S Rosa; Alane Beatriz Vermelho
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The Analyst
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2019
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2025-11-10T15:53:40.966962
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16464699
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pubmed_async
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literature
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Application of SSCP-PCR fingerprinting to profile the yeast community in raw milk Salers cheeses.
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Bacteria and yeasts are important sensory factors of raw-milk cheeses as they contribute to the sensory richness and diversity of these products. The diversity and succession of yeast populations in three traditional Registered Designation of Origin (R.D.O.) Salers cheeses have been determined by using phenotypic diagnoses and Single-Strand Conformation Polymorphism (SSCP) analysis. Isolates were identified by phenotypic tests and the sequencing of the D1-D2 domains of the 26S rRNA gene. Ninety-two percent of the isolates were identified as the same species in both tests. Yeast-specific primers were designed to amplify the V4 region of the 18S rRNA gene for SSCP analysis. The yeast species most frequently encountered in the three cheeses were Kluyveromyces lactis, Kluyveromyces marxianus, Saccharomyces cerevisiae, Candida zeylanoides and Debaryomyces hansenii. Detection of less common species, including Candida parapsilosis, Candida silvae, Candida intermedia, Candida rugosa, Saccharomyces unisporus, and Pichia guilliermondii was more efficient with the conventional method. SSCP analysis was accurate and could be used to rapidly assess the proportions and dynamics of the various species during cheese ripening. Each cheese was clearly distinguished by its own microbial community dynamics.
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16464699
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10.1016/j.syapm.2005.07.008
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Cécile Callon; Céline Delbès; Frédérique Duthoit; Marie-Christine Montel
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Systematic and applied microbiology
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2006
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2025-11-10T15:53:40.967234
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34960188
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pubmed_async
|
literature
|
A Single Vaccination of IBDV Subviral Particles Generated by
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Infectious bursal disease (IBD), caused by the infectious bursal disease virus (IBDV), is a highly contagious and immunosuppressive disease in chickens worldwide. The novel variant IBDV (nvIBDV) has been emerging in Chinese chicken farms since 2017, but there are no available vaccines that can provide effective protection. Herein, the capsid protein VP2 from nvIBDV strain FJ-18 was expressed in
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34960188
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10.1016/j.vaccine.2016.02.072
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Deqiang Yang; Lixia Zhang; Jinkun Duan; Qiang Huang; Yao Yu; Jungang Zhou; Hong Lu
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Vaccines
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2021
|
2025-11-10T15:53:40.967576
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30744615
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pubmed_async
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literature
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Production of high titer of citric acid from inulin.
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Citric acid is considered as the most economically feasible product of microbiological production, therefore studies on cheap and renewable raw materials for its production are highly desirable. In this study citric acid was synthesized by genetically engineered strains of Yarrowia lipolytica from widely available, renewable polysaccharide - inulin. Hydrolysis of inulin by the Y. lipolytica strains was established by expressing the inulinase gene (INU1 gene; GenBank: X57202.1) with its native secretion signal sequence was amplified from genomic DNA from Kluyveromyces marxianus CBS6432. To ensure the maximum citric acid titer, the optimal cultivation strategy-repeated-batch culture was applied. The strain Y. lipolytica AWG7 INU 8 secreted more than 200 g dm The citric acid titer obtained in the proposed process is the highest value reported in the literature for Yarrowia yeast. The obtained results suggest that citric acid production from inulin by engineered Y. lipolytica may be a very promising technology for industrial citric acid production.
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30744615
|
10.1021/ac60147a030
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Magdalena Rakicka; Jakub Wolniak; Zbigniew Lazar; Waldemar Rymowicz
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BMC biotechnology
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2019
|
2025-11-10T15:53:40.967919
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17143639
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pubmed_async
|
literature
|
Continuous ethanol fermentation of cheese whey powder solution: effects of hydraulic residence time.
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Continuous ethanol fermentation of cheese whey powder solution was realized using pure culture of Kluyveromyces marxianus (DSMZ 7239) at hydraulic residence times (HRT) between 12.5 and 60 h. Sugar utilization, ethanol and biomass formation were investigated as functions of HRT. Effluent sugar concentration decreased, but percent sugar utilization, ethanol and biomass concentrations increased with HRT. Ethanol productivity was maximum (0.745 gE l(-1)h(-1)) at an HRT of 43.2 h where the biomass productivity was almost minimum (0.18 gX l(-1) h(-1)). The ethanol yield coefficient was almost constant at 0.4 gE g(-1)S up to HRT of 43.2 h and the growth yield coefficient was minimum at HRT of 43.2 h. Kinetic models were developed and the constants were determined by using the experimental data.
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17143639
|
10.1007/s00449-006-0101-0
|
Serpil Ozmihci; Fikret Kargi
|
Bioprocess and biosystems engineering
|
2007
|
2025-11-10T15:53:40.968425
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Cell 8: Ultimate Full-Text Collection
📊 Dataset Statistics
- Total Records: 236
- PMC Articles: 236 (with FULL-TEXT)
- EuropePMC Articles: 0 (with FULL-TEXT)
🔍 Features
- Complete full-text extraction
- Structured sections (Introduction, Methods, Results, Discussion)
- Biological entity recognition (genes, proteins, enzymes)
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from datasets import load_dataset
dataset = load_dataset("Milad96/Kluyveromyces-marxianus", name="cell8_data")
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